Restriction map for
pF1KA1342
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# NO CUT ENZYMES ( 148 enzymes) #
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AatII Acc65I AccI AccII AciI
AclI AcyI AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BanI BanII BbeI BbvCI
BcgI BclI BglI BlnI BlpI
Bpu1102I BsaBI BsaHI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoT22I FauI FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MluNI MscI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccIII 958 TCCGGA T^CCGGA
AfaI 993 GTAC GT^AC
AflII 169 CTTAAG C^TTAAG
AvaI 783 CTCGGG C^[CT]CG[GA]G
AvaII 1145 GGTCC G^G[AT]CC
BamHI 645 GGATCC G^GATCC
BbrPI 1258 CACGTG CAC^GTG
BciVI 680 GGATAC GTATCC(6/5)
BcnI 21 CCGGG CC^[GC]GG
BfaI 623 CTAG C^TAG
BfrI 169 CTTAAG C^TTAAG
BpmI 352 CTGGAG CTGGAG(16/14)
BsaAI 1258 CACGTG [CT]AC^GT[GA]
BsaI 661 GAGACC GGTCTC(1/5)
BsaWI 958 TCCGGA [AT]^CCGG[AT]
BseAI 958 TCCGGA T^CCGGA
BseRI 143 CTCCTC GAGGAG(10/8)
BsgI 1181 GTGCAG GTGCAG(16/14)
BsmFI 1146 GTCCC GGGAC(10/14)
BsmI 357 GAATGC GAATGC(1/-1)
BsoBI 783 CTCGGG C^[CT]CG[GA]G
BspEI 958 TCCGGA T^CCGGA
BsrDI 272 CATTGC GCAATG(2/0)
BstXI 44 CCACAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 414 TTTAAA TTT^AAA
EcoRI 267 GAATTC G^AATTC
EcoRV 754 GATATC GAT^ATC
MaeI 623 CTAG C^TAG
MaeII 1259 ACGT A^CGT
MroI 958 TCCGGA T^CCGGA
MslI 45 CACAGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 254 CAGCTG C[AC]G^C[GT]G
MwoI 252 GCCAGCTGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 21 CCGGG CC^[GC]GG
NcoI 545 CCATGG C^CATGG
NspI 162 GCATGT [GA]CATG^[CT]
PflMI 699 CCAAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1258 CACGTG CAC^GTG
PmlI 1258 CACGTG CAC^GTG
PpuMI 1144 GGGTCCC [GA]G^G[AT]CC[CT]
PvuII 254 CAGCTG CAG^CTG
RsaI 993 GTAC GT^AC
SfaNI 276 GCATC GCATC(5/9)
SmlI 169 CTTAAG C^T[CT][GA]AG
Tsp45I 1226 GTGAC ^GT[GC]AC
Van91I 699 CCAAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 27 enzymes) #
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AspHI 1207 GAGCAC G[AT]GC[AT]^C
1261 GTGCTC
BbvI 1183 GCAGC GCAGC(8/12)
1186 GCAGC
BglII 297 AGATCT A^GATCT
1220 AGATCT
BmyI 1207 GAGCAC G[AGT]GC[ACT]^C
1261 GTGCTC
BsiHKAI 1207 GAGCAC G[AT]GC[AT]^C
1261 GTGCTC
Bsp1286I 1207 GAGCAC G[AGT]GC[ACT]^C
1261 GTGCTC
BsrI 1198 ACTGG ACTGG(1/-1)
1211 ACTGG
BstF5I 125 CATCC GGATG(2/0)
549 GGATG
Cfr13I 1095 GGGCC G^G[ACGT]CC
1145 GGTCC
DraII 1094 AGGGCCT [GA]G^G[ACGT]CC[CT]
1144 GGGTCCC
DsaI 531 CCGTGG C^C[GA][CT]GG
545 CCATGG
EarI 469 CTCTTC CTCTTC(1/4)
442 GAAGAG
EcoO109I 1094 AGGGCCT [GA]G^G[ACGT]CC[CT]
1144 GGGTCCC
EcoT14I 545 CCATGG C^C[AT][AT]GG
641 CCTTGG
Fnu4HI 1183 GCAGC GC^[ACGT]GC
1186 GCAGC
FokI 125 CATCC GGATG(9/13)
549 GGATG
HapII 21 CCGG C^CGG
959 CCGG
HpaII 21 CCGG C^CGG
959 CCGG
HphI 865 GGTGA GGTGA(8/7)
11 TCACC
ItaI 1183 GCAGC GC^[ACGT]GC
1186 GCAGC
Ksp632I 469 CTCTTC CTCTTC(1/4)
442 GAAGAG
MaeIII 870 GTTAC ^GT[ACGT]AC
1226 GTGAC
MspI 21 CCGG C^CGG
959 CCGG
Sau96I 1095 GGGCC G^G[ACGT]CC
1145 GGTCC
StyI 545 CCATGG C^C[AT][AT]GG
641 CCTTGG
TfiI 1010 GAATC G^A[AT]TC
1132 GATTC
TseI 1183 GCAGC G^C[AT]GC
1186 GCAGC
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# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AcsI AluI AlwI ApoI BbsI
BpuAI BsaJI BsiYI BslI BsmAI
BssKI BstNI BstYI Cac8I DdeI
DpnII EcoRII HaeIII HinfI MboI
MboII MflI MnlI MseI MvaI
NdeII NlaIII NlaIV PleI Sau3AI
ScrFI TaqI Tru9I Tsp509I TspRI
TthHB8I XhoII