Restriction map for
pF1KA1361
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# NO CUT ENZYMES ( 84 enzymes) #
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AclI AflII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AspEI
AviII AvrII BamHI BbeI BbrPI
BfrI BglI BlnI BsgI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspHI
BsrBI BsrFI BsrGI BssHII Bst1107I
BstZ17I Cfr10I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRI EcoT22I FauI FseI FspI
KasI KspI MfeI MluI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NsiI PacI PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SgrAI SnaBI SpeI Sse8387I SspBI
StuI SwaI Van91I XbaI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AatII 1814 GACGTC GACGT^C
Acc65I 2859 GGTACC G^GTACC
AccI 195 GTCTAC GT^[AC][GT]AC
AccII 2494 CGCG CG^CG
AccIII 2519 TCCGGA T^CCGGA
AcsI 1603 AAATTT [GA]^AATT[CT]
AocI 2689 CCTGAGG CC^T[ACGT]AGG
ApoI 1603 AAATTT [GA]^AATT[CT]
AseI 2335 ATTAAT AT^TAAT
AsnI 2335 ATTAAT AT^TAAT
Asp700I 75 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2859 GGTACC G^GTACC
AspI 1894 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 2859 GGTACC G^G[CT][GA]CC
BbsI 1109 GTCTTC GAAGAC(2/6)
BbvCI 2467 GCTGAGG CCTCAGC(-5/-2)
BcgI 1343 CGAGGCAAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2181 GGATAC GTATCC(6/5)
BlpI 2314 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2314 GCTGAGC GC^T[ACGT]AGC
BpuAI 1109 GTCTTC GAAGAC(2/6)
BsaAI 287 TACGTG [CT]AC^GT[GA]
BsaBI 1468 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2519 TCCGGA [AT]^CCGG[AT]
BseAI 2519 TCCGGA T^CCGGA
BspEI 2519 TCCGGA T^CCGGA
BsrDI 688 GCAATG GCAATG(2/0)
BstBI 1682 TTCGAA TT^CGAA
BstEII 2833 GGTCACC G^GT[ACGT]ACC
BstPI 2833 GGTCACC G^GT[ACGT]ACC
BstUI 2494 CGCG CG^CG
Bsu36I 2689 CCTGAGG CC^T[ACGT]AGG
CelII 2314 GCTGAGC GC^T[ACGT]AGC
Eco81I 2689 CCTGAGG CC^T[ACGT]AGG
EcoNI 945 CCTTTTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2833 GGTCACC G^GT[ACGT]ACC
EcoRV 451 GATATC GAT^ATC
HaeII 851 AGCGCC [GA]GCGC^[CT]
HgaI 1875 GCGTC GACGC(5/10)
HincII 1102 GTTAAC GT[CT]^[GA]AC
HindII 1102 GTTAAC GT[CT]^[GA]AC
HpaI 1102 GTTAAC GTT^AAC
KpnI 2859 GGTACC GGTAC^C
MamI 1468 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2519 TCCGGA T^CCGGA
MslI 1164 CACAGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 2494 CGCG CG^CG
NruI 2493 TCGCGA TCG^CGA
NspV 1682 TTCGAA TT^CGAA
PflFI 1894 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 2335 ATTAAT AT^TAAT
SfuI 1682 TTCGAA TT^CGAA
SmaI 2712 CCCGGG CCC^GGG
SphI 1944 GCATGC GCATG^C
SspI 317 AATATT AAT^ATT
Tth111I 1894 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2712 CCCGGG C^CCGGG
XmaI 2712 CCCGGG C^CCGGG
XmnI 75 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcyI 1814 GACGTC G[GA]^CG[CT]C
1874 GGCGTC
AflIII 640 ACATGT A^C[GA][CT]GT
2990 ACATGT
BalI 102 TGGCCA TGG^CCA
161 TGGCCA
BanII 49 GAGCTC G[GA]GC[CT]^C
2053 GAGCTC
BclI 2030 TGATCA T^GATCA
2325 TGATCA
BsaHI 1814 GACGTC G[GA]^CG[CT]C
1874 GGCGTC
BseRI 1702 GAGGAG GAGGAG(10/8)
1906 GAGGAG
BsmFI 2925 GGGAC GGGAC(10/14)
1659 GTCCC
BspLU11I 640 ACATGT A^CATGT
2990 ACATGT
BssSI 134 CACGAG CACGAG(-5/-1)
2955 CACGAG
BstXI 149 CCAATGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2788 CCACAAGCTTGG
DraI 825 TTTAAA TTT^AAA
1194 TTTAAA
DraIII 395 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
1162 CACACAGTG
FbaI 2030 TGATCA T^GATCA
2325 TGATCA
Hin1I 1814 GACGTC G[GA]^CG[CT]C
1874 GGCGTC
MluNI 102 TGGCCA TGG^CCA
161 TGGCCA
MscI 102 TGGCCA TGG^CCA
161 TGGCCA
MspA1I 2065 CAGCTG C[AC]G^C[GT]G
2410 CAGCTG
PaeR7I 1654 CTCGAG C^TCGAG
2864 CTCGAG
PvuII 2065 CAGCTG CAG^CTG
2410 CAGCTG
SacI 49 GAGCTC GAGCT^C
2053 GAGCTC
XhoI 1654 CTCGAG C^TCGAG
2864 CTCGAG
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# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BbvI BcnI
BfaI BglII BmyI BpmI BsaI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmI BsoBI Bsp1286I BspMI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII