Restriction map for
pF1KA1372
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# NO CUT ENZYMES ( 88 enzymes) #
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AccI AccII AclI AcsI AflII
AgeI ApoI AscI AseI AsnI
AspI AvrII BamHI BbrPI BbvCI
BciVI BclI BfrI BglII BlnI
BsaAI BsaBI BsiEI BsiWI BsmI
BspDI BspHI BssHII BssSI Bst1107I
BstEII BstPI BstUI BstZ17I ClaI
CpoI DraI DraIII DrdI EagI
EclXI Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HindIII KspI
MamI MfeI MluI MunI MvnI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI ScaI SfiI
SgrAI SnaBI SpeI SphI SwaI
Tth111I XcmI XhoI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 1669 GACGTC GACGT^C
Acc65I 1862 GGTACC G^GTACC
AccIII 2012 TCCGGA T^CCGGA
AflIII 1447 ACATGT A^C[GA][CT]GT
AhdI 7 GACTGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1933 CCTAAGG CC^T[ACGT]AGG
Aor51HI 1004 AGCGCT AGC^GCT
ApaI 1981 GGGCCC GGGCC^C
Asp700I 126 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1862 GGTACC G^GTACC
AspEI 7 GACTGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1637 TGCGCA TGC^GCA
BbeI 1649 GGCGCC GGCGC^C
BglI 1019 GCCGCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 517 GGTCTC GGTCTC(1/5)
BseAI 2012 TCCGGA T^CCGGA
Bsp1407I 1163 TGTACA T^GTACA
BspEI 2012 TCCGGA T^CCGGA
BspLU11I 1447 ACATGT A^CATGT
BsrFI 1961 ACCGGC [GA]^CCGG[CT]
BsrGI 1163 TGTACA T^GTACA
BstAPI 533 GCAACACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1900 TTCGAA TT^CGAA
BstXI 293 CCAGCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1933 CCTAAGG CC^T[ACGT]AGG
Cfr10I 1961 ACCGGC [GA]^CCGG[CT]
Eam1105I 7 GACTGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1004 AGCGCT AGC^GCT
Eco81I 1933 CCTAAGG CC^T[ACGT]AGG
EcoNI 2028 CCTGCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 1637 TGCGCA TGC^GCA
HpaI 1486 GTTAAC GTT^AAC
KasI 1649 GGCGCC G^GCGCC
KpnI 1862 GGTACC GGTAC^C
MroI 2012 TCCGGA T^CCGGA
NarI 1649 GGCGCC GG^CGCC
NcoI 251 CCATGG C^CATGG
NspV 1900 TTCGAA TT^CGAA
SexAI 1481 ACCTGGT A^CC[AT]GGT
SfoI 1649 GGCGCC GGC^GCC
SfuI 1900 TTCGAA TT^CGAA
SspBI 1163 TGTACA T^GTACA
SspI 34 AATATT AAT^ATT
TfiI 1903 GAATC G^A[AT]TC
Tsp509I 120 AATT ^AATT
XbaI 2005 TCTAGA T^CTAGA
XmnI 126 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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Alw44I 1085 GTGCAC G^TGCAC
1747 GTGCAC
AlwNI 424 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
625 CAGCCCCTG
ApaLI 1085 GTGCAC G^TGCAC
1747 GTGCAC
BalI 491 TGGCCA TGG^CCA
1716 TGGCCA
BcgI 1539 CGAGTCGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
92 GCATCTTGATCG
BfaI 664 CTAG C^TAG
2006 CTAG
BlpI 1372 GCTGAGC GC^T[ACGT]AGC
1581 GCTGAGC
Bpu1102I 1372 GCTGAGC GC^T[ACGT]AGC
1581 GCTGAGC
BsaWI 338 TCCGGT [AT]^CCGG[AT]
2012 TCCGGA
BsmBI 948 CGTCTC CGTCTC(1/5)
1671 CGTCTC
BsrDI 51 CATTGC GCAATG(2/0)
2216 GCAATG
CelII 1372 GCTGAGC GC^T[ACGT]AGC
1581 GCTGAGC
DsaI 251 CCATGG C^C[GA][CT]GG
1713 CCGTGG
HgaI 720 GACGC GACGC(5/10)
577 GCGTC
HincII 547 GTCAAC GT[CT]^[GA]AC
1486 GTTAAC
HindII 547 GTCAAC GT[CT]^[GA]AC
1486 GTTAAC
MaeI 664 CTAG C^TAG
2006 CTAG
MluNI 491 TGGCCA TGG^CCA
1716 TGGCCA
MscI 491 TGGCCA TGG^CCA
1716 TGGCCA
MseI 80 TTAA T^TAA
1487 TTAA
MslI 1414 CACCCGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1878 CACTCGGGTG
SapI 456 GAAGAGC GCTCTTC(1/4)
2000 GCTCTTC
SbfI 1554 CCTGCAGG CCTGCA^GG
2206 CCTGCAGG
SmlI 181 CTCAAG C^T[CT][GA]AG
829 CTCAAG
Sse8387I 1554 CCTGCAGG CCTGCA^GG
2206 CCTGCAGG
Tru9I 80 TTAA T^TAA
1487 TTAA
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# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AspHI AvaI AvaII BanI BanII
BbsI BbvI BcnI BmyI BpmI
BpuAI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI StuI StyI TaqI TseI
Tsp45I TspRI TthHB8I Van91I XhoII
XmaCI XmaI