Restriction map for
pF1KA1385
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AvaII AvrII BanI BbeI
BbrPI BcgI BclI BcnI BfrI
BglI BlnI BlpI Bpu1102I BsaBI
BsaHI BsaI BseAI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
BstBI BstUI Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoT22I FbaI FseI
HaeII HgaI Hin1I HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MspA1I MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 2027 GTATAC GT^[AC][GT]AC
AcsI 1337 GAATTC [GA]^AATT[CT]
AlwNI 1750 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1431 GGGCCC GGGCC^C
ApoI 1337 GAATTC [GA]^AATT[CT]
Asp700I 457 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 2108 TGCGCA TGC^GCA
BalI 1412 TGGCCA TGG^CCA
BamHI 1950 GGATCC G^GATCC
BbvCI 786 CCTCAGC CCTCAGC(-5/-2)
BciVI 1720 GTATCC GTATCC(6/5)
BglII 120 AGATCT A^GATCT
BsaWI 1011 TCCGGT [AT]^CCGG[AT]
BseRI 584 CTCCTC GAGGAG(10/8)
BsiEI 5 CGACCG CG[GA][CT]^CG
BsmI 457 GAATGC GAATGC(1/-1)
Bst1107I 2027 GTATAC GTA^TAC
BstAPI 712 GCAGCCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2053 CCACTACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2027 GTATAC GTA^TAC
CfoI 2109 GCGC GCG^C
DraII 1431 GGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 1724 CCATGG C^C[GA][CT]GG
EcoO109I 1431 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRI 1337 GAATTC G^AATTC
FauI 1227 GCGGG CCCGC(4/6)
FspI 2108 TGCGCA TGC^GCA
HapII 1012 CCGG C^CGG
HhaI 2109 GCGC GCG^C
HinP1I 2109 GCGC G^CGC
HincII 1512 GTCAAC GT[CT]^[GA]AC
HindII 1512 GTCAAC GT[CT]^[GA]AC
HpaII 1012 CCGG C^CGG
MluNI 1412 TGGCCA TGG^CCA
MscI 1412 TGGCCA TGG^CCA
MslI 927 CACCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 1012 CCGG C^CGG
NcoI 1724 CCATGG C^CATGG
PstI 707 CTGCAG CTGCA^G
SacI 2161 GAGCTC GAGCT^C
StuI 388 AGGCCT AGG^CCT
XcmI 1619 CCACGATCAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 457 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BanII 1431 GGGCCC G[GA]GC[CT]^C
2161 GAGCTC
BsaAI 506 TACGTG [CT]AC^GT[GA]
2155 TACGTG
BsgI 1922 CTGCAC GTGCAG(16/14)
876 GTGCAG
BsiYI 391 CCTGTATGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1012 CCGGTGCTTGG
BslI 391 CCTGTATGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1012 CCGGTGCTTGG
BsmFI 151 GGGAC GGGAC(10/14)
1021 GGGAC
BsrDI 717 CATTGC GCAATG(2/0)
340 GCAATG
BstEII 1612 GGTTACC G^GT[ACGT]ACC
1896 GGTCACC
BstF5I 329 GGATG GGATG(2/0)
2184 GGATG
BstPI 1612 GGTTACC G^GT[ACGT]ACC
1896 GGTCACC
EcoO65I 1612 GGTTACC G^GT[ACGT]ACC
1896 GGTCACC
EcoRV 922 GATATC GAT^ATC
1366 GATATC
FokI 329 GGATG GGATG(9/13)
2184 GGATG
MaeII 507 ACGT A^CGT
2156 ACGT
SmlI 1067 CTCAAG C^T[CT][GA]AG
1744 CTCAAG
SphI 377 GCATGC GCATG^C
2105 GCATGC
TaqI 920 TCGA T^CGA
1566 TCGA
TthHB8I 920 TCGA T^CGA
1566 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaJI BsiHKAI BsmAI Bsp1286I
BspMI BsrI BssKI BssSI BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EaeI EarI EcoRII EcoT14I Fnu4HI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII