Restriction map for
pF1KA1452
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# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI AspEI AspI
AvrII BbeI BbrPI BcgI BfaI
BfrI BlnI BsaWI BseAI BsiWI
BsmBI BsmI BspEI BspHI BspLU11I
BsrDI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I DraI Eam1105I
Eco47III EcoO65I EcoRI EcoRV EcoT22I
FseI HindIII HpaI KasI KspI
MaeI MfeI MroI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspI SwaI
Tth111I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 60 enzymes) #
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Acc65I 2099 GGTACC G^GTACC
AccII 1416 CGCG CG^CG
AcsI 1964 AAATTT [GA]^AATT[CT]
AcyI 436 GACGCC G[GA]^CG[CT]C
AflIII 1415 ACGCGT A^C[GA][CT]GT
AocI 636 CCTGAGG CC^T[ACGT]AGG
ApaI 903 GGGCCC GGGCC^C
ApoI 1964 AAATTT [GA]^AATT[CT]
Asp718I 2099 GGTACC G^GTACC
AviII 818 TGCGCA TGC^GCA
BamHI 1379 GGATCC G^GATCC
BbsI 499 GAAGAC GAAGAC(2/6)
BbvCI 1036 GCTGAGG CCTCAGC(-5/-2)
BciVI 1274 GTATCC GTATCC(6/5)
BclI 1938 TGATCA T^GATCA
BglII 2034 AGATCT A^GATCT
BlpI 1731 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1731 GCTGAGC GC^T[ACGT]AGC
BpuAI 499 GAAGAC GAAGAC(2/6)
BsaAI 270 CACGTA [CT]AC^GT[GA]
BsaHI 436 GACGCC G[GA]^CG[CT]C
BsaI 262 GAGACC GGTCTC(1/5)
BsiEI 535 CGGCCG CG[GA][CT]^CG
Bsp1407I 27 TGTACA T^GTACA
BsrBI 1426 GAGCGG CCGCTC(-3/-3)
BsrFI 1840 GCCGGT [GA]^CCGG[CT]
BsrGI 27 TGTACA T^GTACA
BstAPI 1254 GCAGCACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1416 CGCG CG^CG
BstXI 53 CCAAGAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 636 CCTGAGG CC^T[ACGT]AGG
CelII 1731 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1840 GCCGGT [GA]^CCGG[CT]
CpoI 1808 CGGACCG CG^G[AT]CCG
DraIII 722 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2112 GACAGTACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 535 CGGCCG C^GGCCG
EclXI 535 CGGCCG C^GGCCG
Eco52I 535 CGGCCG C^GGCCG
Eco81I 636 CCTGAGG CC^T[ACGT]AGG
EcoNI 423 CCTGGATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1938 TGATCA T^GATCA
FspI 818 TGCGCA TGC^GCA
Hin1I 436 GACGCC G[GA]^CG[CT]C
HincII 2013 GTTGAC GT[CT]^[GA]AC
HindII 2013 GTTGAC GT[CT]^[GA]AC
KpnI 2099 GGTACC GGTAC^C
MluI 1415 ACGCGT A^CGCGT
MvnI 1416 CGCG CG^CG
NcoI 1553 CCATGG C^CATGG
PflMI 942 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsrII 1808 CGGACCG CG^G[AT]CCG
SapI 308 GCTCTTC GCTCTTC(1/4)
ScaI 2061 AGTACT AGT^ACT
SphI 1814 GCATGC GCATG^C
SspBI 27 TGTACA T^GTACA
StuI 1688 AGGCCT AGG^CCT
TfiI 916 GATTC G^A[AT]TC
TspRI 726 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 942 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
Asp700I 1146 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1686 GAAGGCCTTC
AvaI 1606 CTCGGG C^[CT]CG[GA]G
1676 CTCGGG
BsoBI 1606 CTCGGG C^[CT]CG[GA]G
1676 CTCGGG
BspDI 37 ATCGAT AT^CGAT
913 ATCGAT
BspMI 620 ACCTGC ACCTGC(4/8)
671 ACCTGC
ClaI 37 ATCGAT AT^CGAT
913 ATCGAT
HaeII 560 AGCGCC [GA]GCGC^[CT]
1745 AGCGCC
MseI 509 TTAA T^TAA
2105 TTAA
SacI 367 GAGCTC GAGCT^C
709 GAGCTC
Tru9I 509 TTAA T^TAA
2105 TTAA
Tsp509I 1965 AATT ^AATT
1978 AATT
XcmI 911 CCATCGATTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1661 CCAGCACCCCTGTGG
XmnI 1146 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1686 GAAGGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaII BalI BanI BanII
BbvI BcnI BglI BmyI BpmI
BsaBI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MamI MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TseI Tsp45I TthHB8I XhoII