Restriction map for
pF1KA1480
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# NO CUT ENZYMES ( 105 enzymes) #
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AatII Acc65I AccII AcsI AflII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BalI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsgI BsiWI
Bsp1407I BspDI BsrBI BsrGI BssHII
BstBI BstEII BstPI BstUI CelII
ClaI CpoI DraI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoT22I FbaI FseI FspI HindIII
HinfI HpaI KpnI KspI MamI
MfeI MluI MluNI MscI MunI
MvnI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PleI
PmeI PshAI PshBI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI StuI SwaI TaqI
TfiI Tth111I TthHB8I XbaI XhoI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AcyI 2087 GGCGCC G[GA]^CG[CT]C
AflIII 1787 ACATGT A^C[GA][CT]GT
Asp700I 243 GAAACGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1881 GAGCAC G[AT]GC[AT]^C
BamHI 1584 GGATCC G^GATCC
BbeI 2087 GGCGCC GGCGC^C
BbrPI 991 CACGTG CAC^GTG
BciVI 1830 GGATAC GTATCC(6/5)
BsaAI 991 CACGTG [CT]AC^GT[GA]
BsaHI 2087 GGCGCC G[GA]^CG[CT]C
BsiEI 233 CGATCG CG[GA][CT]^CG
BsiHKAI 1881 GAGCAC G[AT]GC[AT]^C
BsmBI 1176 CGTCTC CGTCTC(1/5)
BsmI 1923 GAATGC GAATGC(1/-1)
BspLU11I 1787 ACATGT A^CATGT
Bst1107I 1251 GTATAC GTA^TAC
BstAPI 2343 GCAGACATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 1251 GTATAC GTA^TAC
DraIII 72 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1168 GACTATTCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1891 CGGCCA [CT]^GGCC[GA]
EarI 1309 CTCTTC CTCTTC(1/4)
EcoNI 1480 CCTATGGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 382 GATATC GAT^ATC
HgaI 758 GCGTC GACGC(5/10)
Hin1I 2087 GGCGCC G[GA]^CG[CT]C
KasI 2087 GGCGCC G^GCGCC
Ksp632I 1309 CTCTTC CTCTTC(1/4)
NarI 2087 GGCGCC GG^CGCC
PmaCI 991 CACGTG CAC^GTG
PmlI 991 CACGTG CAC^GTG
PpuMI 1933 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 2341 CTGCAG CTGCA^G
PvuI 233 CGATCG CGAT^CG
ScaI 616 AGTACT AGT^ACT
SfoI 2087 GGCGCC GGC^GCC
SmaI 2417 CCCGGG CCC^GGG
SmlI 1111 CTCAAG C^T[CT][GA]AG
SphI 1359 GCATGC GCATG^C
SspI 691 AATATT AAT^ATT
XmaCI 2417 CCCGGG C^CCGGG
XmaI 2417 CCCGGG C^CCGGG
XmnI 243 GAAACGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 28 enzymes) #
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AccI 490 GTCTAC GT^[AC][GT]AC
1251 GTATAC
AccIII 287 TCCGGA T^CCGGA
1827 TCCGGA
AclI 245 AACGTT AA^CGTT
2044 AACGTT
AocI 1138 CCTGAGG CC^T[ACGT]AGG
1210 CCTGAGG
ApaI 2132 GGGCCC GGGCC^C
2184 GGGCCC
BbsI 415 GAAGAC GAAGAC(2/6)
932 GTCTTC
BbvCI 2116 CCTCAGC CCTCAGC(-5/-2)
96 GCTGAGG
BglI 2017 GCCGTGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2097 GCCCCTGCGGC
BpuAI 415 GAAGAC GAAGAC(2/6)
932 GTCTTC
BsaWI 287 TCCGGA [AT]^CCGG[AT]
1827 TCCGGA
BseAI 287 TCCGGA T^CCGGA
1827 TCCGGA
BspEI 287 TCCGGA T^CCGGA
1827 TCCGGA
BspMI 1778 ACCTGC ACCTGC(4/8)
1905 ACCTGC
BssSI 2197 CACGAG CACGAG(-5/-1)
1982 CTCGTG
Bsu36I 1138 CCTGAGG CC^T[ACGT]AGG
1210 CCTGAGG
Eco81I 1138 CCTGAGG CC^T[ACGT]AGG
1210 CCTGAGG
HincII 130 GTCAAC GT[CT]^[GA]AC
1093 GTCAAC
HindII 130 GTCAAC GT[CT]^[GA]AC
1093 GTCAAC
MroI 287 TCCGGA T^CCGGA
1827 TCCGGA
MseI 2002 TTAA T^TAA
2042 TTAA
NspI 1359 GCATGC [GA]CATG^[CT]
1787 ACATGT
PflMI 201 CCAATACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1323 CCAGTGGGTGG
Psp1406I 245 AACGTT AA^CGTT
2044 AACGTT
PvuII 335 CAGCTG CAG^CTG
837 CAGCTG
SfcI 1911 CTACAG C^T[GA][CT]AG
2341 CTGCAG
Tru9I 2002 TTAA T^TAA
2042 TTAA
Tsp509I 1183 AATT ^AATT
2000 AATT
Van91I 201 CCAATACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1323 CCAGTGGGTGG
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# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaI
AvaII BanI BanII BbvI BcgI
BcnI BfaI BmyI BpmI BsaI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspHI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TseI Tsp45I TspRI
XcmI XhoII