Restriction map for
pF1KA1492
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# NO CUT ENZYMES ( 115 enzymes) #
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AatII AccIII AclI AcyI AflII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI Asp700I AspEI AvaI
AvrII BalI BbeI BbrPI BcgI
BclI BcnI BfrI BglI BlnI
BlpI Bpu1102I BsaHI BsaWI BseAI
BsgI BsiEI BsiWI BsoBI BspDI
BspEI BsrBI BsrFI BssHII BssSI
BstAPI BstBI CelII Cfr10I ClaI
CpoI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoRV FbaI FseI HaeII HapII
HgaI Hin1I HincII HindII HpaI
HpaII KasI MfeI MluI MluNI
MroI MscI MspI MunI NaeI
NarI NciI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PstI PvuI RsrII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I StuI SwaI
Van91I XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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Acc65I 759 GGTACC G^GTACC
AflIII 282 ACATGT A^C[GA][CT]GT
AocI 826 CCTAAGG CC^T[ACGT]AGG
Asp718I 759 GGTACC G^GTACC
AspI 508 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2 TGCGCA TGC^GCA
BamHI 1990 GGATCC G^GATCC
BanI 759 GGTACC G^G[CT][GA]CC
BbsI 193 GAAGAC GAAGAC(2/6)
BbvCI 2030 CCTCAGC CCTCAGC(-5/-2)
BglII 1259 AGATCT A^GATCT
BpuAI 193 GAAGAC GAAGAC(2/6)
BsaAI 1074 TACGTG [CT]AC^GT[GA]
BsaBI 1254 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1027 GAGACC GGTCTC(1/5)
BseRI 1096 GAGGAG GAGGAG(10/8)
BsmBI 1115 GAGACG CGTCTC(1/5)
BsmI 687 GCATTC GAATGC(1/-1)
BspHI 1388 TCATGA T^CATGA
BspLU11I 282 ACATGT A^CATGT
BsrDI 608 CATTGC GCAATG(2/0)
Bst1107I 435 GTATAC GTA^TAC
BstEII 710 GGTCACC G^GT[ACGT]ACC
BstPI 710 GGTCACC G^GT[ACGT]ACC
BstXI 968 CCAAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 435 GTATAC GTA^TAC
Bsu36I 826 CCTAAGG CC^T[ACGT]AGG
Cac8I 1697 GCCAGC GC[ACGT]^[ACGT]GC
CfoI 3 GCGC GCG^C
DraII 1469 GGGTCCC [GA]G^G[ACGT]CC[CT]
Eco81I 826 CCTAAGG CC^T[ACGT]AGG
EcoNI 826 CCTAAGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1469 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 710 GGTCACC G^GT[ACGT]ACC
EcoT22I 1371 ATGCAT ATGCA^T
FauI 20 GCGGG CCCGC(4/6)
FspI 2 TGCGCA TGC^GCA
HhaI 3 GCGC GCG^C
HinP1I 3 GCGC G^CGC
HindIII 1975 AAGCTT A^AGCTT
KpnI 759 GGTACC GGTAC^C
KspI 18 CCGCGG CCGC^GG
MamI 1254 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 767 CCATGG C^CATGG
NsiI 1371 ATGCAT ATGCA^T
NspI 282 ACATGT [GA]CATG^[CT]
PflFI 508 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1469 GGGTCCC [GA]G^G[AT]CC[CT]
RcaI 1388 TCATGA T^CATGA
SacI 898 GAGCTC GAGCT^C
SacII 18 CCGCGG CCGC^GG
SspI 1511 AATATT AAT^ATT
TaqI 1827 TCGA T^CGA
Tsp45I 711 GTCAC ^GT[GC]AC
Tth111I 508 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1827 TCGA T^CGA
XbaI 1111 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccI 435 GTATAC GT^[AC][GT]AC
2236 GTCTAC
AccII 19 CGCG CG^CG
525 CGCG
AseI 2136 ATTAAT AT^TAAT
2190 ATTAAT
AsnI 2136 ATTAAT AT^TAAT
2190 ATTAAT
AspHI 898 GAGCTC G[AT]GC[AT]^C
1302 GAGCAC
BanII 898 GAGCTC G[GA]GC[CT]^C
1099 GAGCCC
BfaI 470 CTAG C^TAG
1112 CTAG
BsiHKAI 898 GAGCTC G[AT]GC[AT]^C
1302 GAGCAC
BsmAI 1027 GAGAC GTCTC(1/5)
1115 GAGAC
Bsp1407I 1334 TGTACA T^GTACA
1402 TGTACA
BspMI 832 GCAGGT ACCTGC(4/8)
2232 GCAGGT
BsrGI 1334 TGTACA T^GTACA
1402 TGTACA
BstUI 19 CGCG CG^CG
525 CGCG
BstYI 1259 AGATCT [GA]^GATC[CT]
1990 GGATCC
HaeIII 528 GGCC GG^CC
1696 GGCC
MaeI 470 CTAG C^TAG
1112 CTAG
MflI 1259 AGATCT [GA]^GATC[CT]
1990 GGATCC
MvnI 19 CGCG CG^CG
525 CGCG
NdeI 404 CATATG CA^TATG
446 CATATG
PshBI 2136 ATTAAT AT^TAAT
2190 ATTAAT
SapI 2268 GAAGAGC GCTCTTC(1/4)
1663 GCTCTTC
SfcI 1034 CTACAG C^T[GA][CT]AG
2286 CTACAG
SspBI 1334 TGTACA T^GTACA
1402 TGTACA
XhoII 1259 AGATCT [GA]^GATC[CT]
1990 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaII BbvI BciVI
BmyI BpmI BsaJI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cfr13I DdeI DpnII DraI
DsaI EarI EcoRII EcoT14I Fnu4HI
FokI HinfI HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MvaI MwoI
NdeII NlaIII NlaIV PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TfiI Tru9I TseI Tsp509I
TspRI XcmI