Restriction map for
pF1KA1533
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# NO CUT ENZYMES ( 100 enzymes) #
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AatII Acc65I AclI AcsI AflII
AgeI AocI Aor51HI ApoI AscI
AseI AsnI Asp718I AviII BamHI
BbeI BbrPI BcgI BciVI BclI
BfrI BsaAI BsiWI BsmBI Bsp1407I
BspDI BspHI BsrDI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
Eco47III Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KasI KpnI
KspI MfeI MluI MunI NarI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
TfiI TthHB8I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccI 910 GTAGAC GT^[AC][GT]AC
AcyI 925 GACGCC G[GA]^CG[CT]C
AflIII 469 ACATGT A^C[GA][CT]GT
AhdI 621 GACGCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2035 GTGCAC G^TGCAC
ApaLI 2035 GTGCAC G^TGCAC
AspEI 621 GACGCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 700 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 201 CCTAGG C^CTAGG
BbvCI 1773 CCTCAGC CCTCAGC(-5/-2)
BfaI 202 CTAG C^TAG
BglI 987 GCCTGACGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 518 AGATCT A^GATCT
BlnI 201 CCTAGG C^CTAGG
BsaBI 444 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 925 GACGCC G[GA]^CG[CT]C
BsaI 436 GAGACC GGTCTC(1/5)
BsiEI 1116 CGGCCG CG[GA][CT]^CG
BspLU11I 469 ACATGT A^CATGT
BspMI 906 GCAGGT ACCTGC(4/8)
BssSI 1557 CTCGTG CACGAG(-5/-1)
EagI 1116 CGGCCG C^GGCCG
Eam1105I 621 GACGCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1116 CGGCCG C^GGCCG
Eco52I 1116 CGGCCG C^GGCCG
Hin1I 925 GACGCC G[GA]^CG[CT]C
MaeI 202 CTAG C^TAG
MamI 444 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 11 TTAA T^TAA
NdeI 5 CATATG CA^TATG
NspI 469 ACATGT [GA]CATG^[CT]
PflFI 700 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
SexAI 647 ACCTGGT A^CC[AT]GGT
Tru9I 11 TTAA T^TAA
Tsp509I 2018 AATT ^AATT
Tth111I 700 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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AccIII 71 TCCGGA T^CCGGA
293 TCCGGA
AfaI 249 GTAC GT^AC
1287 GTAC
BalI 834 TGGCCA TGG^CCA
1955 TGGCCA
BsaWI 71 TCCGGA [AT]^CCGG[AT]
293 TCCGGA
BseAI 71 TCCGGA T^CCGGA
293 TCCGGA
BsgI 522 CTGCAC GTGCAG(16/14)
2107 CTGCAC
BsmI 603 GAATGC GAATGC(1/-1)
1754 GCATTC
BspEI 71 TCCGGA T^CCGGA
293 TCCGGA
BsrBI 1156 GAGCGG CCGCTC(-3/-3)
1563 GAGCGG
EcoNI 369 CCTGCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1953 CCTGGCCAAGG
MluNI 834 TGGCCA TGG^CCA
1955 TGGCCA
MroI 71 TCCGGA T^CCGGA
293 TCCGGA
MscI 834 TGGCCA TGG^CCA
1955 TGGCCA
MslI 129 CACCATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1224 CACAGACGTG
NcoI 131 CCATGG C^CATGG
879 CCATGG
PpuMI 181 GGGACCC [GA]G^G[AT]CC[CT]
733 GGGACCC
RsaI 249 GTAC GT^AC
1287 GTAC
SapI 1173 GCTCTTC GCTCTTC(1/4)
1887 GCTCTTC
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# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
ApaI Asp700I AspHI AvaI AvaII
BanI BanII BbsI BbvI BcnI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MnlI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV PflMI
PleI PstI PvuII SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
StyI TseI Tsp45I TspRI Van91I
XhoII XmaCI XmaI XmnI