Restriction map for
pF1KA1584
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI AocI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BalI BanI BanII BbeI BbrPI
BcgI BglI BlnI BlpI Bpu1102I
BsaHI BsaI BsaWI BseAI BsiEI
BsiYI BslI BsmAI BsmBI BsoBI
BspDI BspEI BspHI BsrBI BsrDI
BsrFI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraII DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI FseI
FspI HaeII HaeIII HgaI HhaI
Hin1I HinP1I HindIII KasI KpnI
KspI MfeI MluI MluNI MroI
MscI MslI MunI MvnI NaeI
NarI NgoMIV NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SpeI SphI Sse8387I StuI
SwaI Tth111I Van91I XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 2869 GTAGAC GT^[AC][GT]AC
AciI 2192 CCGC CCGC(-3/-1)
AflII 2091 CTTAAG C^TTAAG
Alw44I 188 GTGCAC G^TGCAC
AlwNI 260 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 188 GTGCAC G^TGCAC
Asp700I 1159 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 717 GGATCC G^GATCC
BbsI 906 GAAGAC GAAGAC(2/6)
BbvCI 566 CCTCAGC CCTCAGC(-5/-2)
BcnI 2016 CCGGG CC^[GC]GG
BfrI 2091 CTTAAG C^TTAAG
BglII 298 AGATCT A^GATCT
BpuAI 906 GAAGAC GAAGAC(2/6)
BsaAI 1884 TACGTA [CT]AC^GT[GA]
BseRI 31 GAGGAG GAGGAG(10/8)
BsgI 2456 GTGCAG GTGCAG(16/14)
BsiWI 1886 CGTACG C^GTACG
Bsp1407I 1390 TGTACA T^GTACA
BspLU11I 1161 ACATGT A^CATGT
BsrGI 1390 TGTACA T^GTACA
BssSI 362 CACGAG CACGAG(-5/-1)
BstXI 2409 CCAGGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 922 TTTAAA TTT^AAA
DsaI 43 CCATGG C^C[GA][CT]GG
EarI 28 GAAGAG CTCTTC(1/4)
EcoRV 108 GATATC GAT^ATC
FauI 2191 CCCGC CCCGC(4/6)
HapII 2016 CCGG C^CGG
HpaI 151 GTTAAC GTT^AAC
HpaII 2016 CCGG C^CGG
Ksp632I 28 GAAGAG CTCTTC(1/4)
MspA1I 569 CAGCTG C[AC]G^C[GT]G
MspI 2016 CCGG C^CGG
NciI 2016 CCGGG CC^[GC]GG
NcoI 43 CCATGG C^CATGG
NheI 621 GCTAGC G^CTAGC
PstI 1405 CTGCAG CTGCA^G
PvuII 569 CAGCTG CAG^CTG
SnaBI 1884 TACGTA TAC^GTA
SspBI 1390 TGTACA T^GTACA
XbaI 295 TCTAGA T^CTAGA
XmnI 1159 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AflIII 1161 ACATGT A^C[GA][CT]GT
2490 ACACGT
AspHI 188 GTGCAC G[AT]GC[AT]^C
2161 GAGCAC
AvaII 439 GGACC G^G[AT]CC
1528 GGACC
BciVI 411 GGATAC GTATCC(6/5)
2147 GTATCC
BmyI 188 GTGCAC G[AGT]GC[ACT]^C
2161 GAGCAC
BsaBI 1238 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2615 GATTAGCATC
BsiHKAI 188 GTGCAC G[AT]GC[AT]^C
2161 GAGCAC
Bsp1286I 188 GTGCAC G[AGT]GC[ACT]^C
2161 GAGCAC
BspMI 1036 ACCTGC ACCTGC(4/8)
457 GCAGGT
BstYI 298 AGATCT [GA]^GATC[CT]
717 GGATCC
Cfr13I 439 GGACC G^G[ACGT]CC
1528 GGACC
EcoT22I 1348 ATGCAT ATGCA^T
1836 ATGCAT
MamI 1238 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2615 GATTAGCATC
MflI 298 AGATCT [GA]^GATC[CT]
717 GGATCC
NsiI 1348 ATGCAT ATGCA^T
1836 ATGCAT
NspI 1161 ACATGT [GA]CATG^[CT]
1417 ACATGC
Sau96I 439 GGACC G^G[ACGT]CC
1528 GGACC
SexAI 339 ACCTGGT A^CC[AT]GGT
1197 ACCTGGT
Tsp45I 601 GTGAC ^GT[GC]AC
1085 GTCAC
XhoII 298 AGATCT [GA]^GATC[CT]
717 GGATCC
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# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbvI BclI BfaI BpmI BsaJI
BsmFI BsmI BsrI BssKI BstF5I
BstNI Cac8I DdeI DpnII EcoRII
EcoT14I FbaI Fnu4HI FokI HincII
HindII HinfI HphI ItaI MaeI
MaeII MaeIII MboI MboII MnlI
MseI MvaI MwoI NdeI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I