Restriction map for
pF1KA1621
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# NO CUT ENZYMES ( 91 enzymes) #
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AatII Acc65I AclI AflII AflIII
AgeI AhdI AscI AseI AsnI
Asp718I AspEI AvrII BalI BamHI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BpmI BsaBI
BsiWI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstZ17I Cfr10I
ClaI CpoI DraI DraIII Eam1105I
EcoT22I FbaI FseI HindIII HpaI
KpnI MamI MfeI MluI MluNI
MscI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI PacI PaeR7I PflMI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SalI ScaI
SfiI SgrAI SnaBI SpeI SphI
SspBI StuI SwaI Van91I XbaI
XhoI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 2062 GTCTAC GT^[AC][GT]AC
AccIII 1123 TCCGGA T^CCGGA
AcsI 550 GAATTC [GA]^AATT[CT]
Alw44I 990 GTGCAC G^TGCAC
AlwNI 1112 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 639 AGCGCT AGC^GCT
ApaI 2187 GGGCCC GGGCC^C
ApaLI 990 GTGCAC G^TGCAC
ApoI 550 GAATTC [GA]^AATT[CT]
BbeI 79 GGCGCC GGCGC^C
BbrPI 1975 CACGTG CAC^GTG
BsaAI 1975 CACGTG [CT]AC^GT[GA]
BsaI 1509 GGTCTC GGTCTC(1/5)
BseAI 1123 TCCGGA T^CCGGA
BsiEI 1736 CGGCCG CG[GA][CT]^CG
BsmBI 789 CGTCTC CGTCTC(1/5)
BspEI 1123 TCCGGA T^CCGGA
BsrBI 2014 CCGCTC CCGCTC(-3/-3)
BsrI 241 ACTGG ACTGG(1/-1)
BstBI 925 TTCGAA TT^CGAA
BstXI 1760 CCAAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1113 AGATCC [GA]^GATC[CT]
DrdI 168 GACAGAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1736 CGGCCG C^GGCCG
EclXI 1736 CGGCCG C^GGCCG
Eco47III 639 AGCGCT AGC^GCT
Eco52I 1736 CGGCCG C^GGCCG
EcoNI 886 CCTATGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 550 GAATTC G^AATTC
EcoRV 1320 GATATC GAT^ATC
HincII 165 GTTGAC GT[CT]^[GA]AC
HindII 165 GTTGAC GT[CT]^[GA]AC
KasI 79 GGCGCC G^GCGCC
KspI 1605 CCGCGG CCGC^GG
MflI 1113 AGATCC [GA]^GATC[CT]
MroI 1123 TCCGGA T^CCGGA
NarI 79 GGCGCC GG^CGCC
NspV 925 TTCGAA TT^CGAA
PmaCI 1975 CACGTG CAC^GTG
PmlI 1975 CACGTG CAC^GTG
PvuII 1813 CAGCTG CAG^CTG
SacII 1605 CCGCGG CCGC^GG
SapI 2097 GCTCTTC GCTCTTC(1/4)
SbfI 1002 CCTGCAGG CCTGCA^GG
SfoI 79 GGCGCC GGC^GCC
SfuI 925 TTCGAA TT^CGAA
Sse8387I 1002 CCTGCAGG CCTGCA^GG
SspI 345 AATATT AAT^ATT
Tsp509I 551 AATT ^AATT
XcmI 1907 CCAAGCACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1113 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 31 enzymes) #
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AfaI 1686 GTAC GT^AC
1809 GTAC
AocI 1545 CCTCAGG CC^T[ACGT]AGG
2182 CCTGAGG
AspI 1041 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2229 GACCCTGTC
AviII 950 TGCGCA TGC^GCA
1871 TGCGCA
BanII 1831 GAGCCC G[GA]GC[CT]^C
2187 GGGCCC
BbsI 1137 GAAGAC GAAGAC(2/6)
1159 GAAGAC
BlpI 60 GCTGAGC GC^T[ACGT]AGC
1887 GCTGAGC
Bpu1102I 60 GCTGAGC GC^T[ACGT]AGC
1887 GCTGAGC
BpuAI 1137 GAAGAC GAAGAC(2/6)
1159 GAAGAC
BsaWI 426 ACCGGA [AT]^CCGG[AT]
1123 TCCGGA
BspMI 1490 ACCTGC ACCTGC(4/8)
1005 GCAGGT
Bsu36I 1545 CCTCAGG CC^T[ACGT]AGG
2182 CCTGAGG
CelII 60 GCTGAGC GC^T[ACGT]AGC
1887 GCTGAGC
DsaI 1605 CCGCGG C^C[GA][CT]GG
1826 CCACGG
EarI 995 CTCTTC CTCTTC(1/4)
2098 CTCTTC
Eco81I 1545 CCTCAGG CC^T[ACGT]AGG
2182 CCTGAGG
FspI 950 TGCGCA TGC^GCA
1871 TGCGCA
Ksp632I 995 CTCTTC CTCTTC(1/4)
2098 CTCTTC
MseI 325 TTAA T^TAA
628 TTAA
MslI 1269 CACAGATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1308 CATAACAGTG
PflFI 1041 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2229 GACCCTGTC
PstI 1003 CTGCAG CTGCA^G
1693 CTGCAG
RsaI 1686 GTAC GT^AC
1809 GTAC
SexAI 1751 ACCTGGT A^CC[AT]GGT
2066 ACCTGGT
SfcI 1003 CTGCAG C^T[GA][CT]AG
1693 CTGCAG
SmaI 1730 CCCGGG CCC^GGG
1834 CCCGGG
SmlI 229 CTCAAG C^T[CT][GA]AG
1819 CTCAAG
Tru9I 325 TTAA T^TAA
628 TTAA
Tth111I 1041 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2229 GACCCTGTC
XmaCI 1730 CCCGGG C^CCGGG
1834 CCCGGG
XmaI 1730 CCCGGG C^CCGGG
1834 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
Asp700I AspHI AvaI AvaII BanI
BbvI BcnI BfaI BmyI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BssKI BstEII BstF5I BstNI BstPI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI Sau3AI Sau96I
ScrFI SfaNI StyI TaqI TfiI
TseI Tsp45I TspRI TthHB8I XmnI