Restriction map for
pF1KA1664
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# NO CUT ENZYMES ( 68 enzymes) #
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Acc65I AccI AccIII AclI AflII
AhdI AocI AscI AseI AsnI
Asp700I Asp718I AspEI AvrII BcgI
BciVI BfrI BglII BlnI BseAI
BsiWI BspDI BspEI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DraI DrdI Eam1105I
Eco81I EcoO65I EcoRV HgaI HincII
HindII HindIII HpaI KpnI MfeI
MluI MroI MunI NheI NruI
NspV PacI PmeI PshBI Psp1406I
PvuI RsrII SalI SexAI SfiI
SfuI SnaBI SpeI SphI SspI
SwaI XbaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 258 GACGTC GACGT^C
AcsI 2005 GAATTC [GA]^AATT[CT]
AgeI 2132 ACCGGT A^CCGGT
ApaI 349 GGGCCC GGGCC^C
ApoI 2005 GAATTC [GA]^AATT[CT]
AviII 1893 TGCGCA TGC^GCA
BalI 812 TGGCCA TGG^CCA
BlpI 1463 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1463 GCTCAGC GC^T[ACGT]AGC
BsaBI 1938 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1813 GAGACC GGTCTC(1/5)
BsmBI 253 GAGACG CGTCTC(1/5)
BsmI 1535 GCATTC GAATGC(1/-1)
Bsp1407I 2684 TGTACA T^GTACA
BspHI 1577 TCATGA T^CATGA
BspLU11I 2390 ACATGT A^CATGT
BsrGI 2684 TGTACA T^GTACA
CelII 1463 GCTCAGC GC^T[ACGT]AGC
EcoNI 1926 CCTCTGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2005 GAATTC G^AATTC
EcoT22I 2278 ATGCAT ATGCA^T
FspI 1893 TGCGCA TGC^GCA
KspI 51 CCGCGG CCGC^GG
MamI 1938 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 812 TGGCCA TGG^CCA
MscI 812 TGGCCA TGG^CCA
NcoI 1364 CCATGG C^CATGG
NdeI 2728 CATATG CA^TATG
NotI 41 GCGGCCGC GC^GGCCGC
NsiI 2278 ATGCAT ATGCA^T
NspI 2390 ACATGT [GA]CATG^[CT]
PaeR7I 1237 CTCGAG C^TCGAG
PinAI 2132 ACCGGT A^CCGGT
PpuMI 1667 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 1577 TCATGA T^CATGA
SacII 51 CCGCGG CCGC^GG
SapI 2040 GAAGAGC GCTCTTC(1/4)
SbfI 1109 CCTGCAGG CCTGCA^GG
ScaI 1352 AGTACT AGT^ACT
SgrAI 2557 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 383 CCCGGG CCC^GGG
Sse8387I 1109 CCTGCAGG CCTGCA^GG
SspBI 2684 TGTACA T^GTACA
StuI 2570 AGGCCT AGG^CCT
Tsp509I 2006 AATT ^AATT
XhoI 1237 CTCGAG C^TCGAG
XmaCI 383 CCCGGG C^CCGGG
XmaI 383 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 33 enzymes) #
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Aor51HI 118 AGCGCT AGC^GCT
992 AGCGCT
AspI 255 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
2334 GACCCTGTC
BamHI 1610 GGATCC G^GATCC
2655 GGATCC
BbrPI 666 CACGTG CAC^GTG
951 CACGTG
BbvCI 945 CCTCAGC CCTCAGC(-5/-2)
1886 CCTCAGC
BclI 1100 TGATCA T^GATCA
1394 TGATCA
BglI 23 GCCGAGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
523 GCCCCACTGGC
BsaWI 177 ACCGGA [AT]^CCGG[AT]
2132 ACCGGT
BsgI 904 CTGCAC GTGCAG(16/14)
1707 CTGCAC
BsmAI 253 GAGAC GTCTC(1/5)
1813 GAGAC
BspMI 1685 ACCTGC ACCTGC(4/8)
1866 GCAGGT
BsrDI 620 GCAATG GCAATG(2/0)
956 GCAATG
BstAPI 1182 GCAGCGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2585 GCAGCAGCTGC
BstXI 1712 CCAGCTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2010 CCAGGCCAGTGG
DraII 349 GGGCCCT [GA]G^G[ACGT]CC[CT]
1667 AGGACCT
DraIII 1897 CACTACGTG CAC[ACGT][ACGT][ACGT]^GTG
2026 CACTACGTG
Eco47III 118 AGCGCT AGC^GCT
992 AGCGCT
EcoO109I 349 GGGCCCT [GA]G^G[ACGT]CC[CT]
1667 AGGACCT
FbaI 1100 TGATCA T^GATCA
1394 TGATCA
FseI 1264 GGCCGGCC GGCCGG^CC
1268 GGCCGGCC
MaeIII 2191 GTGAC ^GT[ACGT]AC
2207 GTGAC
MseI 439 TTAA T^TAA
1562 TTAA
PflFI 255 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
2334 GACCCTGTC
PflMI 274 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1683 CCACCTGCTGG
PmaCI 666 CACGTG CAC^GTG
951 CACGTG
PmlI 666 CACGTG CAC^GTG
951 CACGTG
PshAI 188 GACCGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
413 GACCCGAGTC
SacI 1461 GAGCTC GAGCT^C
1600 GAGCTC
TfiI 921 GAATC G^A[AT]TC
2022 GAATC
Tru9I 439 TTAA T^TAA
1562 TTAA
Tsp45I 2191 GTGAC ^GT[GC]AC
2207 GTGAC
Tth111I 255 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
2334 GACCCTGTC
Van91I 274 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1683 CCACCTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaLI
AspHI AvaI AvaII BanI BanII
BbeI BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaAI BsaHI
BsaJI BseRI BsiEI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrBI
BsrFI BsrI BssKI BssSI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DsaI
EaeI EagI EarI EclXI Eco52I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI StyI TaqI TseI
TspRI TthHB8I XcmI XhoII