Restriction map for
pF1KA1673
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsiEI
BsiWI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoRV FseI FspI HgaI Hin1I
HindIII HpaI KasI KpnI KspI
MaeII MamI MfeI MluI MluNI
MscI MunI MvnI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI Sse8387I
SspBI StuI SwaI Tth111I Van91I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccIII 1122 TCCGGA T^CCGGA
AfaI 455 GTAC GT^AC
AlwNI 504 CAGTAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1964 GGGCCC GGGCC^C
AspHI 608 GTGCTC G[AT]GC[AT]^C
AvaI 900 CCCGGG C^[CT]CG[GA]G
AvaII 582 GGTCC G^G[AT]CC
BbsI 1236 GTCTTC GAAGAC(2/6)
BclI 1938 TGATCA T^GATCA
BglII 218 AGATCT A^GATCT
BpuAI 1236 GTCTTC GAAGAC(2/6)
BsaI 752 GGTCTC GGTCTC(1/5)
BsaWI 1122 TCCGGA [AT]^CCGG[AT]
BseAI 1122 TCCGGA T^CCGGA
BsgI 1531 GTGCAG GTGCAG(16/14)
BsiHKAI 608 GTGCTC G[AT]GC[AT]^C
BsmBI 488 CGTCTC CGTCTC(1/5)
BsmI 1501 GCATTC GAATGC(1/-1)
BsoBI 900 CCCGGG C^[CT]CG[GA]G
BspEI 1122 TCCGGA T^CCGGA
BsrDI 380 GCAATG GCAATG(2/0)
BssSI 2057 CTCGTG CACGAG(-5/-1)
BstEII 2101 GGTGACC G^GT[ACGT]ACC
BstPI 2101 GGTGACC G^GT[ACGT]ACC
BstYI 218 AGATCT [GA]^GATC[CT]
EaeI 1275 TGGCCG [CT]^GGCC[GA]
EcoO65I 2101 GGTGACC G^GT[ACGT]ACC
EcoRI 2071 GAATTC G^AATTC
FauI 1871 CCCGC CCCGC(4/6)
FbaI 1938 TGATCA T^GATCA
HaeII 525 AGCGCC [GA]GCGC^[CT]
HincII 417 GTTGAC GT[CT]^[GA]AC
HindII 417 GTTGAC GT[CT]^[GA]AC
MflI 218 AGATCT [GA]^GATC[CT]
MroI 1122 TCCGGA T^CCGGA
PleI 867 GAGTC GAGTC(4/5)
PpuMI 581 GGGTCCC [GA]G^G[AT]CC[CT]
RsaI 455 GTAC GT^AC
SmaI 900 CCCGGG CCC^GGG
SphI 1549 GCATGC GCATG^C
SspI 2033 AATATT AAT^ATT
XcmI 2052 CCATTCTCGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 218 AGATCT [GA]^GATC[CT]
XmaCI 900 CCCGGG C^CCGGG
XmaI 900 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
BanII 897 GAGCCC G[GA]GC[CT]^C
1964 GGGCCC
BcnI 900 CCCGG CC^[GC]GG
901 CCGGG
BsmAI 489 GTCTC GTCTC(1/5)
753 GTCTC
CfoI 482 GCGC GCG^C
526 GCGC
DraII 581 GGGTCCC [GA]G^G[ACGT]CC[CT]
1963 GGGGCCC
DsaI 850 CCGTGG C^C[GA][CT]GG
1278 CCGTGG
EarI 888 CTCTTC CTCTTC(1/4)
1292 CTCTTC
EcoNI 1486 CCTCCTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2085 CCTGGTGAAGG
EcoO109I 581 GGGTCCC [GA]G^G[ACGT]CC[CT]
1963 GGGGCCC
EcoT14I 936 CCAAGG C^C[AT][AT]GG
1627 CCTTGG
HapII 901 CCGG C^CGG
1123 CCGG
HhaI 482 GCGC GCG^C
526 GCGC
HinP1I 482 GCGC G^CGC
526 GCGC
HpaII 901 CCGG C^CGG
1123 CCGG
Ksp632I 888 CTCTTC CTCTTC(1/4)
1292 CTCTTC
MspI 901 CCGG C^CGG
1123 CCGG
NciI 900 CCCGG CC^[GC]GG
901 CCGGG
NdeI 1214 CATATG CA^TATG
1925 CATATG
NspI 1145 ACATGC [GA]CATG^[CT]
1549 GCATGC
StyI 936 CCAAGG C^C[AT][AT]GG
1627 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
BbvI BfaI BmyI BpmI BsaJI
BseRI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII EcoRII EcoT22I
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeI MaeIII MboI MboII
MnlI MseI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV NsiI
PstI PvuII Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I