Restriction map for
pF1KA1692
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# NO CUT ENZYMES ( 124 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
Asp700I Asp718I AspEI AviII AvrII
BanI BbeI BbrPI BcgI BciVI
BfaI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BspLU11I BsrFI BsrGI BssHII
Bst1107I BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I FauI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HpaI KasI
KpnI KspI MaeI MfeI MluI
MroI MunI MvnI NaeI NarI
NgoMIV NheI NotI NruI PacI
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PstI PvuI RsrII
SacII SalI SapI SbfI ScaI
SfiI SfoI SgrAI SnaBI SpeI
Sse8387I SspBI StuI SwaI Tsp45I
Van91I XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AflIII 2057 ACGTGT A^C[GA][CT]GT
ApaI 1026 GGGCCC GGGCC^C
AseI 586 ATTAAT AT^TAAT
AsnI 586 ATTAAT AT^TAAT
AspI 213 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1847 GGATCC G^GATCC
BsaBI 1848 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 432 GTGCAG GTGCAG(16/14)
BspHI 335 TCATGA T^CATGA
BsrBI 2343 CCGCTC CCGCTC(-3/-3)
BssSI 44 CACGAG CACGAG(-5/-1)
BstAPI 1951 GCAGGGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2362 TTCGAA TT^CGAA
EcoRI 1150 GAATTC G^AATTC
EcoRV 331 GATATC GAT^ATC
EcoT22I 1 ATGCAT ATGCA^T
HapII 2355 CCGG C^CGG
HindIII 101 AAGCTT A^AGCTT
HpaII 2355 CCGG C^CGG
MamI 1848 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 2355 CCGG C^CGG
NdeI 1679 CATATG CA^TATG
NsiI 1 ATGCAT ATGCA^T
NspV 2362 TTCGAA TT^CGAA
PaeR7I 691 CTCGAG C^TCGAG
PflFI 213 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 53 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 586 ATTAAT AT^TAAT
RcaI 335 TCATGA T^CATGA
SacI 1928 GAGCTC GAGCT^C
SfcI 1619 CTATAG C^T[GA][CT]AG
SfuI 2362 TTCGAA TT^CGAA
SmaI 2354 CCCGGG CCC^GGG
SmlI 691 CTCGAG C^T[CT][GA]AG
SphI 231 GCATGC GCATG^C
SspI 1534 AATATT AAT^ATT
Tth111I 213 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 691 CTCGAG C^TCGAG
XmaCI 2354 CCCGGG C^CCGGG
XmaI 2354 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AspHI 1928 GAGCTC G[AT]GC[AT]^C
1958 GTGCTC
AvaI 691 CTCGAG C^[CT]CG[GA]G
2354 CCCGGG
BclI 492 TGATCA T^GATCA
1716 TGATCA
BcnI 2354 CCCGG CC^[GC]GG
2355 CCGGG
BglII 36 AGATCT A^GATCT
480 AGATCT
BsaI 211 GAGACC GGTCTC(1/5)
282 GAGACC
BseRI 308 CTCCTC GAGGAG(10/8)
1014 CTCCTC
BsiHKAI 1928 GAGCTC G[AT]GC[AT]^C
1958 GTGCTC
BsmI 2415 GAATGC GAATGC(1/-1)
960 GCATTC
BsoBI 691 CTCGAG C^[CT]CG[GA]G
2354 CCCGGG
BspMI 555 ACCTGC ACCTGC(4/8)
1771 ACCTGC
DsaI 1685 CCATGG C^C[GA][CT]GG
1843 CCATGG
EarI 58 CTCTTC CTCTTC(1/4)
904 GAAGAG
FbaI 492 TGATCA T^GATCA
1716 TGATCA
Ksp632I 58 CTCTTC CTCTTC(1/4)
904 GAAGAG
MspA1I 625 CAGCTG C[AC]G^C[GT]G
1504 CAGCTG
NciI 2354 CCCGG CC^[GC]GG
2355 CCGGG
NcoI 1685 CCATGG C^CATGG
1843 CCATGG
PvuII 625 CAGCTG CAG^CTG
1504 CAGCTG
SexAI 932 ACCTGGT A^CC[AT]GGT
2018 ACCAGGT
TaqI 692 TCGA T^CGA
2363 TCGA
TthHB8I 692 TCGA T^CGA
2363 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaII BalI BanII
BbsI BbvCI BbvI BmyI BpmI
BpuAI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TfiI Tru9I TseI Tsp509I
TspRI XhoII