Restriction map for
pF1KA1707
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# NO CUT ENZYMES ( 121 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AvaI
AvrII BalI BanI BbeI BbrPI
BbvCI BcgI BcnI BglI BlnI
BsaHI BsaWI BseAI BseRI BsiEI
BsiWI BsmI BsoBI BspDI BspEI
BspHI BspMI BsrBI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRV
EcoT22I FauI FseI HaeII HgaI
Hin1I KasI KpnI KspI MluI
MluNI MroI MscI MslI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SpeI SphI Sse8387I
StuI SwaI Van91I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AocI 99 CCTAAGG CC^T[ACGT]AGG
AspHI 3004 GAGCTC G[AT]GC[AT]^C
AspI 2622 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 627 TGCGCA TGC^GCA
BclI 723 TGATCA T^GATCA
BglII 2196 AGATCT A^GATCT
BlpI 2566 GCTCAGC GC^T[ACGT]AGC
BpmI 2200 CTGGAG CTGGAG(16/14)
Bpu1102I 2566 GCTCAGC GC^T[ACGT]AGC
BsaAI 2609 TACGTA [CT]AC^GT[GA]
BsaI 1674 GGTCTC GGTCTC(1/5)
BsgI 2636 GTGCAG GTGCAG(16/14)
BsiHKAI 3004 GAGCTC G[AT]GC[AT]^C
BsmBI 2474 CGTCTC CGTCTC(1/5)
BsrFI 1060 GCCGGT [GA]^CCGG[CT]
Bsu36I 99 CCTAAGG CC^T[ACGT]AGG
Cac8I 1730 GCCAGC GC[ACGT]^[ACGT]GC
CelII 2566 GCTCAGC GC^T[ACGT]AGC
CfoI 628 GCGC GCG^C
Cfr10I 1060 GCCGGT [GA]^CCGG[CT]
DraI 1599 TTTAAA TTT^AAA
DsaI 2506 CCATGG C^C[GA][CT]GG
EaeI 2024 CGGCCA [CT]^GGCC[GA]
Eco81I 99 CCTAAGG CC^T[ACGT]AGG
EcoRI 1750 GAATTC G^AATTC
EcoT14I 2506 CCATGG C^C[AT][AT]GG
FbaI 723 TGATCA T^GATCA
FspI 627 TGCGCA TGC^GCA
HapII 1061 CCGG C^CGG
HhaI 628 GCGC GCG^C
HinP1I 628 GCGC G^CGC
HindIII 2904 AAGCTT A^AGCTT
HpaI 2926 GTTAAC GTT^AAC
HpaII 1061 CCGG C^CGG
MaeII 2610 ACGT A^CGT
MfeI 1217 CAATTG C^AATTG
MspA1I 1208 CAGCTG C[AC]G^C[GT]G
MspI 1061 CCGG C^CGG
MunI 1217 CAATTG C^AATTG
NcoI 2506 CCATGG C^CATGG
PflFI 2622 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 2047 CTGCAG CTGCA^G
PvuII 1208 CAGCTG CAG^CTG
SacI 3004 GAGCTC GAGCT^C
ScaI 1321 AGTACT AGT^ACT
SfcI 2047 CTGCAG C^T[GA][CT]AG
SnaBI 2609 TACGTA TAC^GTA
StyI 2506 CCATGG C^C[AT][AT]GG
Tth111I 2622 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 120 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflII 1273 CTTAAG C^TTAAG
2254 CTTAAG
AflIII 334 ACATGT A^C[GA][CT]GT
1360 ACATGT
AvaII 443 GGACC G^G[AT]CC
1243 GGACC
BamHI 945 GGATCC G^GATCC
2916 GGATCC
BanII 579 GGGCTC G[GA]GC[CT]^C
3004 GAGCTC
BfrI 1273 CTTAAG C^TTAAG
2254 CTTAAG
BmyI 579 GGGCTC G[AGT]GC[ACT]^C
3004 GAGCTC
BsaBI 1630 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2599 GATAATGATC
Bsp1286I 579 GGGCTC G[AGT]GC[ACT]^C
3004 GAGCTC
Bsp1407I 1371 TGTACA T^GTACA
2736 TGTACA
BspLU11I 334 ACATGT A^CATGT
1360 ACATGT
BsrDI 302 CATTGC GCAATG(2/0)
1577 CATTGC
BsrGI 1371 TGTACA T^GTACA
2736 TGTACA
Cfr13I 443 GGACC G^G[ACGT]CC
1243 GGACC
DraII 442 AGGACCC [GA]G^G[ACGT]CC[CT]
1242 AGGACCT
EcoO109I 442 AGGACCC [GA]G^G[ACGT]CC[CT]
1242 AGGACCT
HaeIII 2025 GGCC GG^CC
2696 GGCC
HphI 37 TCACC GGTGA(8/7)
175 TCACC
MamI 1630 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2599 GATAATGATC
NspI 334 ACATGT [GA]CATG^[CT]
1360 ACATGT
PpuMI 442 AGGACCC [GA]G^G[AT]CC[CT]
1242 AGGACCT
PshAI 204 GACGAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
402 GACGAATGTC
Sau96I 443 GGACC G^G[ACGT]CC
1243 GGACC
SmlI 1273 CTTAAG C^T[CT][GA]AG
2254 CTTAAG
SspBI 1371 TGTACA T^GTACA
2736 TGTACA
SspI 761 AATATT AAT^ATT
842 AATATT
Tsp45I 2303 GTCAC ^GT[GC]AC
2423 GTCAC
XcmI 1097 CCATTGATATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2095 CCAGATGAAGAATGG
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# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I BbsI BbvI BciVI
BfaI BpuAI BsaJI BsiYI BslI
BsmAI BsmFI BsrI BssKI BstF5I
BstNI BstYI DdeI DpnII EarI
EcoRII Fnu4HI FokI HincII HindII
HinfI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
PleI RsaI Sau3AI ScrFI SfaNI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I XhoII XmnI