Restriction map for
pF1KA1708
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# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AccII AccIII AflII
AgeI Alw44I Aor51HI ApaI ApaLI
AscI Asp718I AviII BalI BamHI
BbrPI BbvCI BfrI BglI BlpI
Bpu1102I BsaBI BsaWI BseAI BsiEI
BsiWI BspEI BsrBI BssHII Bst1107I
BstAPI BstEII BstPI BstUI BstZ17I
CelII CpoI DraIII DrdI DsaI
EagI EclXI Eco47III Eco52I EcoO65I
FseI FspI HgaI KpnI KspI
MamI MluI MluNI MroI MscI
MvnI NcoI NheI NotI NruI
PacI PinAI PmaCI PmeI PmlI
PvuI RsrII SacII SbfI SfiI
SgrAI SmaI SnaBI SphI Sse8387I
SspI SwaI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AclI 1071 AACGTT AA^CGTT
AcyI 7 GGCGCC G[GA]^CG[CT]C
AhdI 4551 GACTGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 115 CCTCAGG CC^T[ACGT]AGG
AseI 3046 ATTAAT AT^TAAT
AsnI 3046 ATTAAT AT^TAAT
Asp700I 2419 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4551 GACTGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2319 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 7 GGCGCC GGCGC^C
BcgI 2568 CGAAACAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 575 CACGTA [CT]AC^GT[GA]
BsaHI 7 GGCGCC G[GA]^CG[CT]C
BsaI 2048 GAGACC GGTCTC(1/5)
BsmBI 1330 GAGACG CGTCTC(1/5)
Bsp1407I 91 TGTACA T^GTACA
BspDI 2947 ATCGAT AT^CGAT
BsrDI 914 GCAATG GCAATG(2/0)
BsrGI 91 TGTACA T^GTACA
BssSI 488 CTCGTG CACGAG(-5/-1)
BstXI 3434 CCACTCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 115 CCTCAGG CC^T[ACGT]AGG
ClaI 2947 ATCGAT AT^CGAT
EaeI 2691 TGGCCG [CT]^GGCC[GA]
Eam1105I 4551 GACTGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 115 CCTCAGG CC^T[ACGT]AGG
EcoNI 3911 CCTCCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 4201 GATATC GAT^ATC
FauI 30 GCGGG CCCGC(4/6)
Hin1I 7 GGCGCC G[GA]^CG[CT]C
HindIII 3385 AAGCTT A^AGCTT
KasI 7 GGCGCC G^GCGCC
MfeI 2224 CAATTG C^AATTG
MunI 2224 CAATTG C^AATTG
NarI 7 GGCGCC GG^CGCC
PaeR7I 344 CTCGAG C^TCGAG
PflFI 2319 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2415 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 464 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 2474 GACCCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3046 ATTAAT AT^TAAT
Psp1406I 1071 AACGTT AA^CGTT
SalI 3161 GTCGAC G^TCGAC
SapI 2455 GCTCTTC GCTCTTC(1/4)
SexAI 2051 ACCAGGT A^CC[AT]GGT
SfoI 7 GGCGCC GGC^GCC
SpeI 3484 ACTAGT A^CTAGT
SspBI 91 TGTACA T^GTACA
StuI 2630 AGGCCT AGG^CCT
Tth111I 2319 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2415 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 344 CTCGAG C^TCGAG
XmnI 2419 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 3161 GTCGAC GT^[AC][GT]AC
4410 GTCTAC
AciI 30 GCGG CCGC(-3/-1)
1553 GCGG
AvaI 344 CTCGAG C^[CT]CG[GA]G
1006 CTCGGG
AvaII 465 GGTCC G^G[AT]CC
1349 GGTCC
AvrII 126 CCTAGG C^CTAGG
4434 CCTAGG
BclI 1374 TGATCA T^GATCA
1785 TGATCA
BcnI 12 CCCGG CC^[GC]GG
632 CCCGG
BlnI 126 CCTAGG C^CTAGG
4434 CCTAGG
BsgI 3054 CTGCAC GTGCAG(16/14)
2555 GTGCAG
BsoBI 344 CTCGAG C^[CT]CG[GA]G
1006 CTCGGG
BspLU11I 957 ACATGT A^CATGT
1259 ACATGT
BsrFI 2643 GCCGGC [GA]^CCGG[CT]
3794 GCCGGC
BstBI 1508 TTCGAA TT^CGAA
4226 TTCGAA
Cfr10I 2643 GCCGGC [GA]^CCGG[CT]
3794 GCCGGC
DraI 424 TTTAAA TTT^AAA
3336 TTTAAA
DraII 464 AGGTCCT [GA]G^G[ACGT]CC[CT]
4437 AGGCCCT
EcoO109I 464 AGGTCCT [GA]G^G[ACGT]CC[CT]
4437 AGGCCCT
EcoRI 527 GAATTC G^AATTC
2609 GAATTC
EcoT22I 1340 ATGCAT ATGCA^T
2576 ATGCAT
FbaI 1374 TGATCA T^GATCA
1785 TGATCA
HaeII 7 GGCGCC [GA]GCGC^[CT]
1539 AGCGCC
HpaI 310 GTTAAC GTT^AAC
4422 GTTAAC
NaeI 2643 GCCGGC GCC^GGC
3794 GCCGGC
NciI 12 CCCGG CC^[GC]GG
632 CCCGG
NgoMIV 2643 GCCGGC G^CCGGC
3794 GCCGGC
NsiI 1340 ATGCAT ATGCA^T
2576 ATGCAT
NspV 1508 TTCGAA TT^CGAA
4226 TTCGAA
PvuII 2558 CAGCTG CAG^CTG
4886 CAGCTG
ScaI 3150 AGTACT AGT^ACT
3316 AGTACT
SfuI 1508 TTCGAA TT^CGAA
4226 TTCGAA
XcmI 2777 CCATTTCCAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3431 CCACCACTCAGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AcsI AfaI AflIII AluI AlwI
AlwNI ApoI AspHI BanI BanII
BbsI BbvI BciVI BfaI BglII
BmyI BpmI BpuAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspHI BspMI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NdeI NdeII
NlaIII NlaIV NspI PleI PstI
RcaI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XbaI XhoII