Restriction map for
pF1KA1747
##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII AccI AclI AcyI AflIII
AhdI AscI AseI AsnI AspEI
AviII AvrII BbeI BbvCI BciVI
BclI BfaI BlnI BlpI Bpu1102I
BsaBI BsaHI BsiWI BsmBI Bsp1407I
BspDI BspHI BspLU11I BsrGI BssHII
BssSI Bst1107I BstBI BstZ17I CelII
ClaI CpoI DraI DrdI Eam1105I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HgaI Hin1I HindIII HpaI
KasI KspI MaeI MamI MfeI
MluI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 230 GGTACC G^GTACC
AccIII 356 TCCGGA T^CCGGA
AcsI 2019 GAATTT [GA]^AATT[CT]
AflII 2245 CTTAAG C^TTAAG
Alw44I 1690 GTGCAC G^TGCAC
AocI 2286 CCTGAGG CC^T[ACGT]AGG
ApaLI 1690 GTGCAC G^TGCAC
ApoI 2019 GAATTT [GA]^AATT[CT]
Asp718I 230 GGTACC G^GTACC
AspI 721 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 1693 CACGTG CAC^GTG
BbsI 1905 GAAGAC GAAGAC(2/6)
BcgI 129 CGAGCAGCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2245 CTTAAG C^TTAAG
BglII 1718 AGATCT A^GATCT
BpuAI 1905 GAAGAC GAAGAC(2/6)
BsaAI 1693 CACGTG [CT]AC^GT[GA]
BseAI 356 TCCGGA T^CCGGA
BsiEI 37 CGGCCG CG[GA][CT]^CG
BsmI 913 GAATGC GAATGC(1/-1)
BspEI 356 TCCGGA T^CCGGA
BsrBI 226 GAGCGG CCGCTC(-3/-3)
BstAPI 1190 GCAAGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 669 GGTCACC G^GT[ACGT]ACC
BstPI 669 GGTCACC G^GT[ACGT]ACC
BstXI 476 CCATTTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2286 CCTGAGG CC^T[ACGT]AGG
DraIII 1424 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 37 CGGCCG C^GGCCG
EclXI 37 CGGCCG C^GGCCG
Eco52I 37 CGGCCG C^GGCCG
Eco81I 2286 CCTGAGG CC^T[ACGT]AGG
EcoO65I 669 GGTCACC G^GT[ACGT]ACC
FauI 1390 CCCGC CCCGC(4/6)
HincII 1768 GTCAAC GT[CT]^[GA]AC
HindII 1768 GTCAAC GT[CT]^[GA]AC
KpnI 230 GGTACC GGTAC^C
MroI 356 TCCGGA T^CCGGA
PflFI 721 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1693 CACGTG CAC^GTG
PmlI 1693 CACGTG CAC^GTG
SexAI 250 ACCAGGT A^CC[AT]GGT
SmaI 1443 CCCGGG CCC^GGG
TaqI 608 TCGA T^CGA
Tsp45I 670 GTCAC ^GT[GC]AC
Tth111I 721 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 608 TCGA T^CGA
XcmI 2255 CCAACAATGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1443 CCCGGG C^CCGGG
XmaI 1443 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 199 CGCG CG^CG
1392 CGCG
AgeI 685 ACCGGT A^CCGGT
1049 ACCGGT
Aor51HI 809 AGCGCT AGC^GCT
1193 AGCGCT
AvaI 127 CCCGAG C^[CT]CG[GA]G
1443 CCCGGG
BamHI 1163 GGATCC G^GATCC
2273 GGATCC
BanI 230 GGTACC G^G[CT][GA]CC
526 GGTGCC
BseRI 1270 CTCCTC GAGGAG(10/8)
458 GAGGAG
BsoBI 127 CCCGAG C^[CT]CG[GA]G
1443 CCCGGG
BsrDI 332 CATTGC GCAATG(2/0)
1598 GCAATG
BstUI 199 CGCG CG^CG
1392 CGCG
Eco47III 809 AGCGCT AGC^GCT
1193 AGCGCT
EcoNI 1221 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2282 CCTCCCTGAGG
HaeII 809 AGCGCT [GA]GCGC^[CT]
1193 AGCGCT
MvnI 199 CGCG CG^CG
1392 CGCG
PflMI 939 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1116 CCAGCAGCTGG
PinAI 685 ACCGGT A^CCGGT
1049 ACCGGT
PpuMI 1234 AGGTCCT [GA]G^G[AT]CC[CT]
1529 AGGACCT
SacI 859 GAGCTC GAGCT^C
2164 GAGCTC
SapI 1662 GAAGAGC GCTCTTC(1/4)
1176 GCTCTTC
SmlI 2126 CTCAAG C^T[CT][GA]AG
2245 CTTAAG
SphI 135 GCATGC GCATG^C
1646 GCATGC
TfiI 1259 GAATC G^A[AT]TC
1946 GAATC
Van91I 939 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1116 CCAGCAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI Asp700I AspHI AvaII BalI
BanII BbvI BcnI BglI BmyI
BpmI BsaI BsaJI BsaWI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI Tru9I
TseI Tsp509I TspRI XhoII XmnI