Restriction map for
pF1KA1757
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# NO CUT ENZYMES ( 75 enzymes) #
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Acc65I AccIII AclI AflII AgeI
ApaI AscI Asp700I Asp718I AspI
AviII AvrII BbeI BbrPI BfrI
BglII BlnI BseAI BsiWI BspDI
BspEI BspMI BsrBI BsrFI BssHII
BstAPI BstBI BstXI Cfr10I ClaI
CpoI DraI EcoRV FseI FspI
HpaI KasI KpnI KspI MfeI
MluI MroI MunI NaeI NarI
NdeI NgoMIV NotI NruI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacI SacII SalI ScaI SfiI
SfoI SfuI SgrAI SmaI SspI
SwaI Tth111I XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 76 enzymes) #
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AatII 1964 GACGTC GACGT^C
AccII 360 CGCG CG^CG
AcyI 1964 GACGTC G[GA]^CG[CT]C
AflIII 1877 ACATGT A^C[GA][CT]GT
AhdI 3828 GACTATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3931 GTGCAC G^TGCAC
AocI 522 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2012 AGCGCT AGC^GCT
ApaLI 3931 GTGCAC G^TGCAC
AseI 424 ATTAAT AT^TAAT
AsnI 424 ATTAAT AT^TAAT
AspEI 3828 GACTATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 2648 CTCGAG C^[CT]CG[GA]G
BalI 2270 TGGCCA TGG^CCA
BanI 2998 GGTGCC G^G[CT][GA]CC
BbsI 1434 GTCTTC GAAGAC(2/6)
BcgI 2991 CGACACTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1098 TGATCA T^GATCA
BglI 1494 GCCTTCTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 3332 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3332 GCTCAGC GC^T[ACGT]AGC
BpuAI 1434 GTCTTC GAAGAC(2/6)
BsaAI 1835 TACGTA [CT]AC^GT[GA]
BsaBI 2557 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1964 GACGTC G[GA]^CG[CT]C
BsaI 3504 GAGACC GGTCTC(1/5)
BsiEI 1507 CGGCCG CG[GA][CT]^CG
BsmBI 1962 GAGACG CGTCTC(1/5)
BsmI 2637 GAATGC GAATGC(1/-1)
BsoBI 2648 CTCGAG C^[CT]CG[GA]G
BspHI 1101 TCATGA T^CATGA
BspLU11I 1877 ACATGT A^CATGT
Bst1107I 114 GTATAC GTA^TAC
BstEII 819 GGTCACC G^GT[ACGT]ACC
BstPI 819 GGTCACC G^GT[ACGT]ACC
BstUI 360 CGCG CG^CG
BstZ17I 114 GTATAC GTA^TAC
Bsu36I 522 CCTGAGG CC^T[ACGT]AGG
CelII 3332 GCTCAGC GC^T[ACGT]AGC
DrdI 1553 GACTAAAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1507 CGGCCG C^GGCCG
Eam1105I 3828 GACTATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1507 CGGCCG C^GGCCG
Eco47III 2012 AGCGCT AGC^GCT
Eco52I 1507 CGGCCG C^GGCCG
Eco81I 522 CCTGAGG CC^T[ACGT]AGG
EcoNI 2728 CCTGCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 819 GGTCACC G^GT[ACGT]ACC
EcoRI 234 GAATTC G^AATTC
EcoT22I 1222 ATGCAT ATGCA^T
FbaI 1098 TGATCA T^GATCA
HaeII 2012 AGCGCT [GA]GCGC^[CT]
HgaI 1560 GCGTC GACGC(5/10)
Hin1I 1964 GACGTC G[GA]^CG[CT]C
HincII 1732 GTCAAC GT[CT]^[GA]AC
HindII 1732 GTCAAC GT[CT]^[GA]AC
HindIII 323 AAGCTT A^AGCTT
MamI 2557 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2270 TGGCCA TGG^CCA
MscI 2270 TGGCCA TGG^CCA
MslI 2704 CATTTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 360 CGCG CG^CG
NcoI 3003 CCATGG C^CATGG
NheI 3700 GCTAGC G^CTAGC
NsiI 1222 ATGCAT ATGCA^T
PaeR7I 2648 CTCGAG C^TCGAG
PshBI 424 ATTAAT AT^TAAT
RcaI 1101 TCATGA T^CATGA
SbfI 3711 CCTGCAGG CCTGCA^GG
SnaBI 1835 TACGTA TAC^GTA
SpeI 1467 ACTAGT A^CTAGT
SphI 1224 GCATGC GCATG^C
Sse8387I 3711 CCTGCAGG CCTGCA^GG
StuI 3869 AGGCCT AGG^CCT
XbaI 1659 TCTAGA T^CTAGA
XhoI 2648 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AspHI 640 GTGCTC G[AT]GC[AT]^C
3931 GTGCAC
BanII 2151 GAGCCC G[GA]GC[CT]^C
3253 GAGCCC
BbvCI 3020 CCTCAGC CCTCAGC(-5/-2)
814 GCTGAGG
BcnI 1427 CCCGG CC^[GC]GG
1505 CCCGG
BsaWI 512 ACCGGA [AT]^CCGG[AT]
890 ACCGGA
BsiHKAI 640 GTGCTC G[AT]GC[AT]^C
3931 GTGCAC
Bsp1407I 351 TGTACA T^GTACA
727 TGTACA
BsrDI 3809 CATTGC GCAATG(2/0)
2099 GCAATG
BsrGI 351 TGTACA T^GTACA
727 TGTACA
CfoI 361 GCGC GCG^C
2013 GCGC
DsaI 3003 CCATGG C^C[GA][CT]GG
3915 CCGTGG
EaeI 1507 CGGCCG [CT]^GGCC[GA]
2270 TGGCCA
FauI 3571 CCCGC CCCGC(4/6)
1260 GCGGG
HhaI 361 GCGC GCG^C
2013 GCGC
HinP1I 361 GCGC G^CGC
2013 GCGC
NciI 1427 CCCGG CC^[GC]GG
1505 CCCGG
NspI 1224 GCATGC [GA]CATG^[CT]
1877 ACATGT
PflMI 3640 CCACATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3910 CCACACCGTGG
PpuMI 3215 AGGTCCT [GA]G^G[AT]CC[CT]
3717 GGGACCC
SapI 642 GCTCTTC GCTCTTC(1/4)
2114 GCTCTTC
SexAI 1884 ACCAGGT A^CC[AT]GGT
2796 ACCTGGT
SmlI 2648 CTCGAG C^T[CT][GA]AG
3140 CTCAAG
SspBI 351 TGTACA T^GTACA
727 TGTACA
Van91I 3640 CCACATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3910 CCACACCGTGG
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# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AvaII BamHI
BbvI BciVI BfaI BmyI BpmI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BssSI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraII DraIII
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII