Restriction map for
pF1KA1762
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# NO CUT ENZYMES ( 87 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AgeI AhdI Aor51HI
ApoI AscI AseI AsnI Asp718I
AspEI AvrII BamHI BbrPI BciVI
BclI BlnI BsaAI BsaBI BseAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BsrGI BssHII Bst1107I BstEII BstPI
BstUI BstZ17I ClaI CpoI DraI
Eam1105I Eco47III EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HgaI HincII
HindII HpaI KpnI KspI MamI
MfeI MluI MroI MunI MvnI
NdeI NotI NruI NsiI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SexAI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SwaI XhoI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AcyI 3519 GGCGCC G[GA]^CG[CT]C
AflII 916 CTTAAG C^TTAAG
AflIII 1350 ACATGT A^C[GA][CT]GT
Asp700I 1308 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 2429 TGCGCA TGC^GCA
BbeI 3519 GGCGCC GGCGC^C
BbvCI 289 GCTGAGG CCTCAGC(-5/-2)
BfrI 916 CTTAAG C^TTAAG
BsaHI 3519 GGCGCC G[GA]^CG[CT]C
BsaWI 1834 ACCGGA [AT]^CCGG[AT]
BsiEI 1629 CGGCCG CG[GA][CT]^CG
BspHI 3086 TCATGA T^CATGA
BspLU11I 1350 ACATGT A^CATGT
DrdI 1730 GACTCGCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1629 CGGCCG C^GGCCG
EclXI 1629 CGGCCG C^GGCCG
Eco52I 1629 CGGCCG C^GGCCG
FspI 2429 TGCGCA TGC^GCA
Hin1I 3519 GGCGCC G[GA]^CG[CT]C
KasI 3519 GGCGCC G^GCGCC
MaeII 266 ACGT A^CGT
NaeI 1627 GCCGGC GCC^GGC
NarI 3519 GGCGCC GG^CGCC
NgoMIV 1627 GCCGGC G^CCGGC
NheI 1753 GCTAGC G^CTAGC
PflMI 4335 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 3852 GACCACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3086 TCATGA T^CATGA
SfoI 3519 GGCGCC GGC^GCC
SmaI 2579 CCCGGG CCC^GGG
SspI 460 AATATT AAT^ATT
StuI 3606 AGGCCT AGG^CCT
Van91I 4335 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1179 TCTAGA T^CTAGA
XmaCI 2579 CCCGGG C^CCGGG
XmaI 2579 CCCGGG C^CCGGG
XmnI 1308 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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Alw44I 426 GTGCAC G^TGCAC
597 GTGCAC
ApaLI 426 GTGCAC G^TGCAC
597 GTGCAC
AspI 3733 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3769 GACAAGGTC
BbsI 262 GAAGAC GAAGAC(2/6)
3849 GAAGAC
BcgI 663 GCATCAGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2501 GCAAGATGCTCG
BglI 1291 GCCTGTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3608 GCCTCCTCGGC
BglII 2265 AGATCT A^GATCT
2934 AGATCT
BpuAI 262 GAAGAC GAAGAC(2/6)
3849 GAAGAC
BsmI 1782 GAATGC GAATGC(1/-1)
3192 GAATGC
BssSI 1342 CACGAG CACGAG(-5/-1)
3197 CTCGTG
BstBI 1406 TTCGAA TT^CGAA
2600 TTCGAA
BstXI 3641 CCACCACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3654 CCAGACTGCTGG
DraIII 429 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
2858 CACCCAGTG
HindIII 2894 AAGCTT A^AGCTT
3104 AAGCTT
MslI 2155 CATACCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3642 CACCACAGTG
NcoI 284 CCATGG C^CATGG
1091 CCATGG
NspV 1406 TTCGAA TT^CGAA
2600 TTCGAA
PflFI 3733 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3769 GACAAGGTC
SacI 316 GAGCTC GAGCT^C
627 GAGCTC
SapI 727 GAAGAGC GCTCTTC(1/4)
1546 GAAGAGC
SfiI 1290 GGCCTGTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3607 GGCCTCCTCGGCC
SfuI 1406 TTCGAA TT^CGAA
2600 TTCGAA
Tsp45I 2162 GTGAC ^GT[GC]AC
2207 GTCAC
Tsp509I 481 AATT ^AATT
3583 AATT
Tth111I 3733 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3769 GACAAGGTC
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# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI ApaI AspHI AvaI AvaII
BalI BanI BanII BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
TspRI TthHB8I XcmI XhoII