Restriction map for
pF1KA1795
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# NO CUT ENZYMES ( 149 enzymes) #
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AatII AccIII AclI AcsI AcyI
AflII AflIII AgeI AhdI AocI
Aor51HI ApaI ApoI AscI Asp700I
AspEI AspI AvaI AviII AvrII
BalI BamHI BbeI BbrPI BbsI
BcgI BciVI BcnI BfrI BglII
BlnI BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I Cfr10I ClaI
CpoI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FseI FspI HaeII HapII HgaI
Hin1I HincII HindII HpaI HpaII
KasI Ksp632I KspI MaeII MamI
MluI MluNI MroI MscI MslI
MspI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tth111I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 387 GGTACC G^GTACC
AccI 954 GTCTAC GT^[AC][GT]AC
AccII 858 CGCG CG^CG
AciI 357 CCGC CCGC(-3/-1)
Alw44I 638 GTGCAC G^TGCAC
AlwNI 941 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 638 GTGCAC G^TGCAC
AseI 1206 ATTAAT AT^TAAT
AsnI 1206 ATTAAT AT^TAAT
Asp718I 387 GGTACC G^GTACC
BanI 387 GGTACC G^G[CT][GA]CC
BanII 936 GGGCTC G[GA]GC[CT]^C
BbvCI 609 CCTCAGC CCTCAGC(-5/-2)
BclI 810 TGATCA T^GATCA
BfaI 227 CTAG C^TAG
BglI 577 GCCTCTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BssHII 857 GCGCGC G^CGCGC
BstAPI 1270 GCACAAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 858 CGCG CG^CG
DraI 756 TTTAAA TTT^AAA
DraII 59 AGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 1271 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 196 GACCTTACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 128 CCACGG C^C[GA][CT]GG
EcoO109I 59 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 522 CCTTGG C^C[AT][AT]GG
FbaI 810 TGATCA T^GATCA
HindIII 399 AAGCTT A^AGCTT
KpnI 387 GGTACC GGTAC^C
MaeI 227 CTAG C^TAG
MfeI 502 CAATTG C^AATTG
MspA1I 512 CAGCTG C[AC]G^C[GT]G
MunI 502 CAATTG C^AATTG
MvnI 858 CGCG CG^CG
NspI 908 GCATGT [GA]CATG^[CT]
PflMI 271 CCATGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 59 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 1206 ATTAAT AT^TAAT
PstI 469 CTGCAG CTGCA^G
PvuII 512 CAGCTG CAG^CTG
ScaI 316 AGTACT AGT^ACT
SmlI 320 CTCAAG C^T[CT][GA]AG
SspI 561 AATATT AAT^ATT
StyI 522 CCTTGG C^C[AT][AT]GG
Van91I 271 CCATGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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AspHI 154 GTGCTC G[AT]GC[AT]^C
638 GTGCAC
BlpI 156 GCTCAGC GC^T[ACGT]AGC
946 GCTGAGC
Bpu1102I 156 GCTCAGC GC^T[ACGT]AGC
946 GCTGAGC
BsaI 342 GAGACC GGTCTC(1/5)
995 GAGACC
BsiHKAI 154 GTGCTC G[AT]GC[AT]^C
638 GTGCAC
BstF5I 408 GGATG GGATG(2/0)
729 GGATG
BstYI 783 GGATCT [GA]^GATC[CT]
1251 AGATCC
CelII 156 GCTCAGC GC^T[ACGT]AGC
946 GCTGAGC
CfoI 857 GCGC GCG^C
859 GCGC
FokI 408 GGATG GGATG(9/13)
729 GGATG
HaeIII 527 GGCC GG^CC
1223 GGCC
HhaI 857 GCGC GCG^C
859 GCGC
HinP1I 857 GCGC G^CGC
859 GCGC
MflI 783 GGATCT [GA]^GATC[CT]
1251 AGATCC
PleI 184 GACTC GAGTC(4/5)
796 GAGTC
TaqI 462 TCGA T^CGA
774 TCGA
Tsp45I 115 GTCAC ^GT[GC]AC
656 GTGAC
TthHB8I 462 TCGA T^CGA
774 TCGA
XhoII 783 GGATCT [GA]^GATC[CT]
1251 AGATCC
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# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AfaI AluI AlwI AvaII BbvI
BmyI BpmI BsaJI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
Fnu4HI HinfI HphI ItaI MaeIII
MboI MboII MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TfiI Tru9I TseI Tsp509I TspRI