Restriction map for
pF1KA1805
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# NO CUT ENZYMES ( 138 enzymes) #
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AatII AccII AccIII AclI AcyI
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I AspEI AspI AviII AvrII
BamHI BbeI BbrPI BciVI BclI
BcnI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiWI BsmI
BspDI BspEI BspLU11I BsrBI BsrFI
BssHII BstAPI BstBI BstEII BstPI
BstUI BstYI CelII CfoI Cfr10I
ClaI CpoI DraIII DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoT22I
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI KasI Ksp632I KspI MamI
MfeI MflI MluI MroI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspI StuI SwaI
Tth111I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 1113 GGTACC G^GTACC
AflII 153 CTTAAG C^TTAAG
AocI 915 CCTGAGG CC^T[ACGT]AGG
Asp718I 1113 GGTACC G^GTACC
AvaI 1543 CCCGAG C^[CT]CG[GA]G
BalI 1073 TGGCCA TGG^CCA
BanI 1113 GGTACC G^G[CT][GA]CC
BbvCI 1390 GCTGAGG CCTCAGC(-5/-2)
BcgI 65 GCACGAGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 988 CTAG C^TAG
BfrI 153 CTTAAG C^TTAAG
BpmI 774 CTCCAG CTGGAG(16/14)
BsaI 1505 GGTCTC GGTCTC(1/5)
BsmBI 1017 GAGACG CGTCTC(1/5)
BsmFI 36 GGGAC GGGAC(10/14)
BsoBI 1543 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1288 TGTACA T^GTACA
BspHI 141 TCATGA T^CATGA
BspMI 517 ACCTGC ACCTGC(4/8)
BsrDI 471 GCAATG GCAATG(2/0)
BsrGI 1288 TGTACA T^GTACA
BssSI 66 CACGAG CACGAG(-5/-1)
Bst1107I 1039 GTATAC GTA^TAC
BstXI 788 CCAGCATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1039 GTATAC GTA^TAC
Bsu36I 915 CCTGAGG CC^T[ACGT]AGG
DraI 552 TTTAAA TTT^AAA
EaeI 1073 TGGCCA [CT]^GGCC[GA]
Eco81I 915 CCTGAGG CC^T[ACGT]AGG
FauI 25 CCCGC CCCGC(4/6)
HapII 683 CCGG C^CGG
HpaII 683 CCGG C^CGG
KpnI 1113 GGTACC GGTAC^C
MaeI 988 CTAG C^TAG
MaeII 1020 ACGT A^CGT
MaeIII 866 GTCAC ^GT[ACGT]AC
MluNI 1073 TGGCCA TGG^CCA
MscI 1073 TGGCCA TGG^CCA
MspI 683 CCGG C^CGG
NspI 758 GCATGC [GA]CATG^[CT]
PstI 836 CTGCAG CTGCA^G
PvuII 254 CAGCTG CAG^CTG
RcaI 141 TCATGA T^CATGA
ScaI 1173 AGTACT AGT^ACT
SexAI 1109 ACCTGGT A^CC[AT]GGT
SmlI 153 CTTAAG C^T[CT][GA]AG
SphI 758 GCATGC GCATG^C
SspBI 1288 TGTACA T^GTACA
TfiI 245 GAATC G^A[AT]TC
Tsp45I 866 GTCAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 1039 GTATAC GT^[AC][GT]AC
1246 GTCTAC
AcsI 343 GAATTT [GA]^AATT[CT]
1342 GAATTT
AlwI 71 GGATC GGATC(4/5)
1575 GGATC
AlwNI 1051 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1105 CAGGACCTG
ApoI 343 GAATTT [GA]^AATT[CT]
1342 GAATTT
AspHI 63 GAGCAC G[AT]GC[AT]^C
102 GTGCTC
BanII 1150 GGGCTC G[GA]GC[CT]^C
1281 GGGCTC
BsiHKAI 63 GAGCAC G[AT]GC[AT]^C
102 GTGCTC
BstF5I 230 GGATG GGATG(2/0)
632 GGATG
EcoRV 800 GATATC GAT^ATC
1195 GATATC
EcoT14I 296 CCAAGG C^C[AT][AT]GG
1076 CCAAGG
FokI 230 GGATG GGATG(9/13)
632 GGATG
HindIII 364 AAGCTT A^AGCTT
1428 AAGCTT
MspA1I 254 CAGCTG C[AC]G^C[GT]G
1456 CAGCGG
PflMI 522 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1076 CCAAGGAATGG
StyI 296 CCAAGG C^C[AT][AT]GG
1076 CCAAGG
Tsp509I 344 AATT ^AATT
1343 AATT
Van91I 522 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1076 CCAAGGAATGG
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# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AfaI AluI AvaII BbsI
BbvI BmyI BpuAI BsaJI BseRI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstNI Cac8I Cfr13I DdeI
DpnII DraII EcoO109I EcoRII Fnu4HI
HaeIII HinfI HphI ItaI MboI
MboII MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI Tru9I TseI
TspRI TthHB8I