##################################
# NO CUT ENZYMES (  173 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        Asp700I     AspEI       AspI        
AvaI        AvaII       AviII       AvrII       BalI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BcnI        BfrI        
BglI        BlnI        BlpI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsgI        BsiEI       BsiWI       
BsiYI       BslI        BsmAI       BsmBI       BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstEII      BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      Cfr13I      ClaI        CpoI        DraI        
DraII       DrdI        DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoT22I     FauI        
FbaI        FseI        FspI        HaeII       HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        HpaII       KasI        
KspI        MaeII       MamI        MfeI        MluI        
MluNI       MroI        MscI        MspA1I      MspI        
MunI        MvnI        NaeI        NarI        NciI        
NcoI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       Psp1406I    PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        Sau96I      SbfI        ScaI        SexAI       
SfiI        SfoI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       StuI        
SwaI        Tsp45I      Tth111I     Van91I      XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

Acc65I        215  GGTACC               G^GTACC
AfaI          216  GTAC                 GT^AC
AseI          118  ATTAAT               AT^TAAT
AsnI          118  ATTAAT               AT^TAAT
Asp718I       215  GGTACC               G^GTACC
AspHI         230  GAGCAC               G[AT]GC[AT]^C
BanI          215  GGTACC               G^G[CT][GA]CC
BglII         583  AGATCT               A^GATCT
BmyI          230  GAGCAC               G[AGT]GC[ACT]^C
BsaJI         824  CCTTGG               C^C[ACGT][ACGT]GG
BsiHKAI       230  GAGCAC               G[AT]GC[AT]^C
BsmFI         196  GGGAC                GGGAC(10/14)
BsmI          165  GAATGC               GAATGC(1/-1)
Bsp1286I      230  GAGCAC               G[AGT]GC[ACT]^C
BssKI         512  CCAGG                ^CC[ACGT]GG
BstBI        1007  TTCGAA               TT^CGAA
BstNI         512  CCAGG                CC^[AT]GG
DraIII        155  CACAGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoRI        1010  GAATTC               G^AATTC
EcoRII        512  CCAGG                ^CC[AT]GG
EcoRV         658  GATATC               GAT^ATC
EcoT14I       824  CCTTGG               C^C[AT][AT]GG
HphI          204  GGTGA                GGTGA(8/7)
KpnI          215  GGTACC               GGTAC^C
MaeIII        795  GTTAC                ^GT[ACGT]AC
MslI          544  CACATTGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI          512  CCAGG                CC^[AT]GG
NdeI          590  CATATG               CA^TATG
NspV         1007  TTCGAA               TT^CGAA
PshBI         118  ATTAAT               AT^TAAT
RsaI          216  GTAC                 GT^AC
ScrFI         512  CCAGG                CC^[ACGT]GG
SfuI         1007  TTCGAA               TT^CGAA
SmlI          898  CTTGAG               C^T[CT][GA]AG
SspI          616  AATATT               AAT^ATT
StyI          824  CCTTGG               C^C[AT][AT]GG
XbaI          580  TCTAGA               T^CTAGA
XcmI          260  CCAACATTAAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

BamHI         406  GGATCC               G^GATCC
              444  GGATCC              
BspMI         400  GCAGGT               ACCTGC(4/8)
              678  GCAGGT              
BsrBI         742  GAGCGG               CCGCTC(-3/-3)
              763  GAGCGG              
Cac8I         228  GCGAGC               GC[ACGT]^[ACGT]GC
              911  GCTTGC              
HaeIII        258  GGCC                 GG^CC
              666  GGCC                
MwoI          600  GCCTCATTTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              744  GCGGCTTCAGC         
PleI          313  GACTC                GAGTC(4/5)
              453  GAGTC               
SfaNI         358  GATGC                GCATC(5/9)
              397  GATGC               
TspRI         157  CAGTG                CA[GC]TG[ACGT][ACGT]^
              321  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (   38 enzymes) #
#########################################

AciI        AcsI        AluI        AlwI        ApoI        
BbvI        BfaI        BseRI       BsrI        BstF5I      
BstYI       DdeI        DpnII       EarI        Fnu4HI      
FokI        HinfI       ItaI        Ksp632I     MaeI        
MboI        MboII       MflI        MnlI        MseI        
NdeII       NlaIII      NlaIV       PstI        Sau3AI      
SfcI        TaqI        TfiI        Tru9I       TseI        
Tsp509I     TthHB8I     XhoII