Restriction map for
pF1KA1847
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII Acc65I AccI AclI AcsI
AflII AgeI AhdI AocI Aor51HI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvrII BamHI
BbrPI BbsI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsgI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspHI BsrBI BsrDI BsrGI BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HincII HindII HindIII HpaI
KpnI KspI MaeII MamI MfeI
MflI MluI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI SspBI SspI SwaI Tsp509I
Tth111I XbaI XhoII XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccIII 295 TCCGGA T^CCGGA
AflIII 1268 ACATGT A^C[GA][CT]GT
Alw44I 931 GTGCAC G^TGCAC
AlwI 298 GGATC GGATC(4/5)
ApaLI 931 GTGCAC G^TGCAC
BalI 674 TGGCCA TGG^CCA
BbeI 73 GGCGCC GGCGC^C
BbvCI 762 GCTGAGG CCTCAGC(-5/-2)
BfaI 1228 CTAG C^TAG
BsaWI 295 TCCGGA [AT]^CCGG[AT]
BseAI 295 TCCGGA T^CCGGA
BspEI 295 TCCGGA T^CCGGA
BspLU11I 1268 ACATGT A^CATGT
BsrI 1065 CCAGT ACTGG(1/-1)
BssHII 404 GCGCGC G^CGCGC
EcoNI 1482 CCTGCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 1408 GCGTC GACGC(5/10)
KasI 73 GGCGCC G^GCGCC
MaeI 1228 CTAG C^TAG
MaeIII 851 GTGAC ^GT[ACGT]AC
MluNI 674 TGGCCA TGG^CCA
MroI 295 TCCGGA T^CCGGA
MscI 674 TGGCCA TGG^CCA
MseI 569 TTAA T^TAA
NarI 73 GGCGCC GG^CGCC
NspI 1268 ACATGT [GA]CATG^[CT]
PaeR7I 1046 CTCGAG C^TCGAG
SbfI 651 CCTGCAGG CCTGCA^GG
SfoI 73 GGCGCC GGC^GCC
SmaI 210 CCCGGG CCC^GGG
SmlI 1046 CTCGAG C^T[CT][GA]AG
Sse8387I 651 CCTGCAGG CCTGCA^GG
StuI 1150 AGGCCT AGG^CCT
Tru9I 569 TTAA T^TAA
Tsp45I 851 GTGAC ^GT[GC]AC
XcmI 1025 CCATCCATGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1046 CTCGAG C^TCGAG
XmaCI 210 CCCGGG C^CCGGG
XmaI 210 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AcyI 73 GGCGCC G[GA]^CG[CT]C
1407 GGCGTC
ApaI 1223 GGGCCC GGGCC^C
1298 GGGCCC
AvaI 210 CCCGGG C^[CT]CG[GA]G
1046 CTCGAG
AviII 42 TGCGCA TGC^GCA
806 TGCGCA
BglI 1234 GCCGGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1291 GCCAAGCGGGC
BsaHI 73 GGCGCC G[GA]^CG[CT]C
1407 GGCGTC
BsoBI 210 CCCGGG C^[CT]CG[GA]G
1046 CTCGAG
BstXI 449 CCATGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
866 CCAGAGTGGTGG
DpnII 299 GATC ^GATC
1335 GATC
FspI 42 TGCGCA TGC^GCA
806 TGCGCA
Hin1I 73 GGCGCC G[GA]^CG[CT]C
1407 GGCGTC
MboI 299 GATC ^GATC
1335 GATC
NcoI 449 CCATGG C^CATGG
591 CCATGG
NdeII 299 GATC ^GATC
1335 GATC
PflMI 1029 CCATGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1065 CCAGTGTGTGG
Sau3AI 299 GATC ^GATC
1335 GATC
TfiI 85 GATTC G^A[AT]TC
334 GAATC
Van91I 1029 CCATGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1065 CCAGTGTGTGG
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# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AspHI AvaII BanI BanII BbvI
BcnI BmyI BpmI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrFI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MboII MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NgoMIV NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI SacI SapI Sau96I ScrFI
SfaNI SfcI StyI TaqI TseI
TspRI TthHB8I