Restriction map for
pF1KA1851
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# NO CUT ENZYMES ( 102 enzymes) #
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AatII AccI AccIII AclI AflII
AgeI AhdI AscI AseI AsnI
Asp700I AspEI AspI BbrPI BciVI
BclI BfrI BglII BsaBI BsaI
BsaWI BseAI BsiWI BsmAI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI Bst1107I BstBI BstEII
BstF5I BstPI BstZ17I ClaI CpoI
DraI DraIII DrdI Eam1105I EcoNI
EcoO65I EcoRV EcoT22I FbaI FokI
FseI HindIII HpaI KspI MamI
MfeI MluI MroI MseI MunI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI SfuI
SgrAI SmaI SpeI Sse8387I SspBI
SspI SwaI TfiI Tru9I Tsp45I
Tth111I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 765 GGTACC G^GTACC
AcsI 1423 GAATTC [GA]^AATT[CT]
AflIII 356 ACACGT A^C[GA][CT]GT
Aor51HI 695 AGCGCT AGC^GCT
ApaI 1066 GGGCCC GGGCC^C
ApoI 1423 GAATTC [GA]^AATT[CT]
Asp718I 765 GGTACC G^GTACC
AvaI 1132 CCCGAG C^[CT]CG[GA]G
AviII 920 TGCGCA TGC^GCA
AvrII 147 CCTAGG C^CTAGG
BamHI 967 GGATCC G^GATCC
BbsI 310 GTCTTC GAAGAC(2/6)
BbvCI 1417 GCTGAGG CCTCAGC(-5/-2)
BlnI 147 CCTAGG C^CTAGG
BpuAI 310 GTCTTC GAAGAC(2/6)
BseRI 570 CTCCTC GAGGAG(10/8)
BsiEI 847 CGGCCG CG[GA][CT]^CG
BsoBI 1132 CCCGAG C^[CT]CG[GA]G
BsrDI 924 CATTGC GCAATG(2/0)
BstAPI 156 GCAGGACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 967 GGATCC [GA]^GATC[CT]
EagI 847 CGGCCG C^GGCCG
EclXI 847 CGGCCG C^GGCCG
Eco47III 695 AGCGCT AGC^GCT
Eco52I 847 CGGCCG C^GGCCG
EcoRI 1423 GAATTC G^AATTC
EcoT14I 147 CCTAGG C^C[AT][AT]GG
FspI 920 TGCGCA TGC^GCA
HincII 316 GTCAAC GT[CT]^[GA]AC
HindII 316 GTCAAC GT[CT]^[GA]AC
HphI 1154 TCACC GGTGA(8/7)
KpnI 765 GGTACC GGTAC^C
MaeIII 174 GTTAC ^GT[ACGT]AC
MflI 967 GGATCC [GA]^GATC[CT]
MslI 984 CATCGCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 618 GCCGGC GCC^GGC
NgoMIV 618 GCCGGC G^CCGGC
PflMI 829 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1497 GGGTCCC [GA]G^G[AT]CC[CT]
SapI 853 GAAGAGC GCTCTTC(1/4)
ScaI 1302 AGTACT AGT^ACT
SexAI 4 ACCTGGT A^CC[AT]GGT
SmlI 628 CTCAAG C^T[CT][GA]AG
SnaBI 745 TACGTA TAC^GTA
StuI 1446 AGGCCT AGG^CCT
StyI 147 CCTAGG C^C[AT][AT]GG
Tsp509I 1424 AATT ^AATT
Van91I 829 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 967 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Alw44I 436 GTGCAC G^TGCAC
1111 GTGCAC
AlwI 968 GATCC GGATC(4/5)
967 GGATC
AlwNI 220 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
1412 CAGTAGCTG
AocI 330 CCTGAGG CC^T[ACGT]AGG
885 CCTGAGG
ApaLI 436 GTGCAC G^TGCAC
1111 GTGCAC
BalI 1010 TGGCCA TGG^CCA
1058 TGGCCA
BanII 1066 GGGCCC G[GA]GC[CT]^C
1129 GAGCCC
BbeI 243 GGCGCC GGCGC^C
960 GGCGCC
BglI 1379 GCCGGAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1388 GCCTCAATGGC
BsaAI 745 TACGTA [CT]AC^GT[GA]
1357 CACGTA
BsiYI 572 CCTCCAACCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
829 CCAGCTCTTGG
BslI 572 CCTCCAACCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
829 CCAGCTCTTGG
BsrBI 1273 CCGCTC CCGCTC(-3/-3)
844 GAGCGG
BsrFI 578 ACCGGC [GA]^CCGG[CT]
618 GCCGGC
BsrI 142 ACTGG ACTGG(1/-1)
1063 ACTGG
BssSI 1093 CACGAG CACGAG(-5/-1)
1189 CTCGTG
BstXI 575 CCAACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1157 CCACACTGCTGG
Bsu36I 330 CCTGAGG CC^T[ACGT]AGG
885 CCTGAGG
Cfr10I 578 ACCGGC [GA]^CCGG[CT]
618 GCCGGC
DsaI 459 CCACGG C^C[GA][CT]GG
1175 CCGTGG
Eco81I 330 CCTGAGG CC^T[ACGT]AGG
885 CCTGAGG
KasI 243 GGCGCC G^GCGCC
960 GGCGCC
MluNI 1010 TGGCCA TGG^CCA
1058 TGGCCA
MscI 1010 TGGCCA TGG^CCA
1058 TGGCCA
NarI 243 GGCGCC GG^CGCC
960 GGCGCC
PstI 154 CTGCAG CTGCA^G
1450 CTGCAG
SfaNI 292 GATGC GCATC(5/9)
983 GCATC
SfiI 1378 GGCCGGAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1387 GGCCTCAATGGCC
SfoI 243 GGCGCC GGC^GCC
960 GGCGCC
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# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaII BanI BbvI BcgI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BsaHI BsaJI BsgI BsiHKAI
BsmFI Bsp1286I BspMI BssHII BssKI
BstNI BstUI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII EaeI
EarI EcoO109I EcoRII FauI Fnu4HI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII ItaI
Ksp632I MaeI MaeII MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfcI SphI TaqI
TseI TspRI TthHB8I