Restriction map for
pF1KA1861
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# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI Asp700I Asp718I
AspEI AviII AvrII BalI BanII
BbeI BbrPI BbvCI BcgI BclI
BglI BglII BlnI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BsrBI
BsrFI BssHII BssSI BstAPI BstBI
BstEII BstPI BstUI CfoI Cfr10I
ClaI CpoI DrdI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI KasI KpnI Ksp632I KspI
MamI MluI MluNI MroI MscI
MvnI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I Van91I XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AciI 2294 CCGC CCGC(-3/-1)
AflII 1861 CTTAAG C^TTAAG
AflIII 921 ACATGT A^C[GA][CT]GT
AocI 1669 CCTGAGG CC^T[ACGT]AGG
AseI 139 ATTAAT AT^TAAT
AsnI 139 ATTAAT AT^TAAT
AspHI 2746 GAGCAC G[AT]GC[AT]^C
AspI 8 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1733 CTCGGG C^[CT]CG[GA]G
BamHI 2800 GGATCC G^GATCC
BcnI 66 CCGGG CC^[GC]GG
BfrI 1861 CTTAAG C^TTAAG
BlpI 987 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 987 GCTCAGC GC^T[ACGT]AGC
BseRI 553 GAGGAG GAGGAG(10/8)
BsgI 866 GTGCAG GTGCAG(16/14)
BsiHKAI 2746 GAGCAC G[AT]GC[AT]^C
BsiYI 72 CCCTGGAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 72 CCCTGGAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 70 GTCCC GGGAC(10/14)
BsmI 1785 GAATGC GAATGC(1/-1)
BsoBI 1733 CTCGGG C^[CT]CG[GA]G
BspHI 1428 TCATGA T^CATGA
BspLU11I 921 ACATGT A^CATGT
BspMI 1238 GCAGGT ACCTGC(4/8)
Bst1107I 2531 GTATAC GTA^TAC
BstXI 2314 CCAATTTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2531 GTATAC GTA^TAC
Bsu36I 1669 CCTGAGG CC^T[ACGT]AGG
CelII 987 GCTCAGC GC^T[ACGT]AGC
DraII 68 GGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 2116 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 2174 CCACGG C^C[GA][CT]GG
Eco81I 1669 CCTGAGG CC^T[ACGT]AGG
EcoO109I 68 GGGTCCC [GA]G^G[ACGT]CC[CT]
HapII 66 CCGG C^CGG
HpaII 66 CCGG C^CGG
MspI 66 CCGG C^CGG
NciI 66 CCGGG CC^[GC]GG
NdeI 799 CATATG CA^TATG
NspI 921 ACATGT [GA]CATG^[CT]
PflFI 8 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 68 GGGTCCC [GA]G^G[AT]CC[CT]
PshBI 139 ATTAAT AT^TAAT
RcaI 1428 TCATGA T^CATGA
ScaI 1526 AGTACT AGT^ACT
StuI 444 AGGCCT AGG^CCT
SwaI 1434 ATTTAAAT ATTT^AAAT
Tth111I 8 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2034 TCTAGA T^CTAGA
XcmI 2174 CCACGGAATCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 1988 GTAGAC GT^[AC][GT]AC
2531 GTATAC
AvaII 69 GGTCC G^G[AT]CC
1714 GGTCC
BanI 49 GGTGCC G^G[CT][GA]CC
2094 GGTGCC
BbsI 398 GAAGAC GAAGAC(2/6)
362 GTCTTC
BciVI 1035 GGATAC GTATCC(6/5)
2139 GGATAC
BmyI 2600 GTGCCC G[AGT]GC[ACT]^C
2746 GAGCAC
BpmI 199 CTGGAG CTGGAG(16/14)
550 CTGGAG
BpuAI 398 GAAGAC GAAGAC(2/6)
362 GTCTTC
Bsp1286I 2600 GTGCCC G[AGT]GC[ACT]^C
2746 GAGCAC
Bsp1407I 385 TGTACA T^GTACA
916 TGTACA
BsrGI 385 TGTACA T^GTACA
916 TGTACA
BstYI 909 GGATCT [GA]^GATC[CT]
2800 GGATCC
Cfr13I 69 GGTCC G^G[ACGT]CC
1714 GGTCC
DraI 616 TTTAAA TTT^AAA
1435 TTTAAA
EcoRI 85 GAATTC G^AATTC
645 GAATTC
EcoT14I 740 CCAAGG C^C[AT][AT]GG
2183 CCTTGG
MfeI 1868 CAATTG C^AATTG
2611 CAATTG
MflI 909 GGATCT [GA]^GATC[CT]
2800 GGATCC
MunI 1868 CAATTG C^AATTG
2611 CAATTG
PleI 1970 GACTC GAGTC(4/5)
60 GAGTC
PstI 272 CTGCAG CTGCA^G
2243 CTGCAG
Sau96I 69 GGTCC G^G[ACGT]CC
1714 GGTCC
SspBI 385 TGTACA T^GTACA
916 TGTACA
SspI 409 AATATT AAT^ATT
2756 AATATT
StyI 740 CCAAGG C^C[AT][AT]GG
2183 CCTTGG
XhoII 909 GGATCT [GA]^GATC[CT]
2800 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI BbvI BfaI BsaJI BsmAI
BsrDI BsrI BssKI BstF5I BstNI
Cac8I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII HindIII HinfI HphI
ItaI MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
PvuII RsaI Sau3AI ScrFI SfaNI
SfcI SmlI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I