Restriction map for
pF1KA1883
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# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AscI AseI AsnI
Asp700I Asp718I AspI AvrII BbrPI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BsaBI BseAI BsiWI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssHII Bst1107I BstBI
BstZ17I ClaI CpoI DraI EcoRI
EcoRV EcoT22I FbaI HindIII HpaI
KpnI MaeI MamI MluI MroI
MseI NdeI NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
RcaI RsrII SalI SapI SexAI
SfuI SgrAI SnaBI SpeI SphI
SspBI SspI SwaI Tru9I Tth111I
XbaI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AcsI 211 GAATTT [GA]^AATT[CT]
AhdI 3001 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 787 GTGCAC G^TGCAC
Aor51HI 2916 AGCGCT AGC^GCT
ApaLI 787 GTGCAC G^TGCAC
ApoI 211 GAATTT [GA]^AATT[CT]
AspEI 3001 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 2327 TGGCCA TGG^CCA
BamHI 911 GGATCC G^GATCC
BsaAI 784 TACGTG [CT]AC^GT[GA]
BsaWI 3459 ACCGGA [AT]^CCGG[AT]
BsmI 771 GCATTC GAATGC(1/-1)
BssSI 3420 CACGAG CACGAG(-5/-1)
BstAPI 1312 GCACACAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 911 GGATCC [GA]^GATC[CT]
DraIII 1313 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 3001 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2916 AGCGCT AGC^GCT
EcoNI 2595 CCTGCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI 1461 GGCCGGCC GGCCGG^CC
MfeI 1213 CAATTG C^AATTG
MflI 911 GGATCC [GA]^GATC[CT]
MluNI 2327 TGGCCA TGG^CCA
MscI 2327 TGGCCA TGG^CCA
MunI 1213 CAATTG C^AATTG
NcoI 2162 CCATGG C^CATGG
NspI 1101 GCATGT [GA]CATG^[CT]
PleI 2119 GACTC GAGTC(4/5)
PshAI 2512 GACCCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 3125 CGATCG CGAT^CG
SbfI 405 CCTGCAGG CCTGCA^GG
ScaI 1610 AGTACT AGT^ACT
Sse8387I 405 CCTGCAGG CCTGCA^GG
StuI 2343 AGGCCT AGG^CCT
XhoII 911 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AocI 1699 CCTCAGG CC^T[ACGT]AGG
3649 CCTCAGG
AviII 806 TGCGCA TGC^GCA
839 TGCGCA
BbsI 1954 GAAGAC GAAGAC(2/6)
3093 GAAGAC
BlpI 1818 GCTGAGC GC^T[ACGT]AGC
4110 GCTGAGC
Bpu1102I 1818 GCTGAGC GC^T[ACGT]AGC
4110 GCTGAGC
BpuAI 1954 GAAGAC GAAGAC(2/6)
3093 GAAGAC
BstEII 1011 GGTGACC G^GT[ACGT]ACC
1401 GGTCACC
BstPI 1011 GGTGACC G^GT[ACGT]ACC
1401 GGTCACC
Bsu36I 1699 CCTCAGG CC^T[ACGT]AGG
3649 CCTCAGG
CelII 1818 GCTGAGC GC^T[ACGT]AGC
4110 GCTGAGC
Eco81I 1699 CCTCAGG CC^T[ACGT]AGG
3649 CCTCAGG
EcoO65I 1011 GGTGACC G^GT[ACGT]ACC
1401 GGTCACC
FspI 806 TGCGCA TGC^GCA
839 TGCGCA
HincII 638 GTCAAC GT[CT]^[GA]AC
4149 GTCAAC
HindII 638 GTCAAC GT[CT]^[GA]AC
4149 GTCAAC
MaeII 785 ACGT A^CGT
4067 ACGT
PaeR7I 710 CTCGAG C^TCGAG
1429 CTCGAG
PvuII 1732 CAGCTG CAG^CTG
2581 CAGCTG
TfiI 80 GATTC G^A[AT]TC
3029 GATTC
Tsp509I 212 AATT ^AATT
1214 AATT
XhoI 710 CTCGAG C^TCGAG
1429 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BanI BanII BbeI
BbvCI BbvI BcnI BglI BmyI
BpmI BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DrdI DsaI EaeI EagI
EarI EclXI Eco52I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I KspI MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
PflMI PpuMI PstI RsaI SacI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SfiI SfoI SmaI SmlI
StyI TaqI TseI Tsp45I TspRI
TthHB8I Van91I XcmI XmaCI XmaI