Restriction map for
pF1KA1885
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# NO CUT ENZYMES ( 92 enzymes) #
##################################
AccIII AclI AflII AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I AspEI AspI AvrII BbrPI
BclI BfrI BlnI BsaBI BseAI
BsiEI BsiWI Bsp1407I BspDI BspEI
BspHI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HpaI KspI MamI
MfeI MluI MroI MunI NaeI
NdeI NgoMIV NheI NotI NsiI
NspV PacI PaeR7I PflFI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AatII 2455 GACGTC GACGT^C
Acc65I 338 GGTACC G^GTACC
AccII 1064 CGCG CG^CG
AflIII 1152 ACATGT A^C[GA][CT]GT
AgeI 1301 ACCGGT A^CCGGT
Asp718I 338 GGTACC G^GTACC
AviII 1626 TGCGCA TGC^GCA
BbeI 2681 GGCGCC GGCGC^C
BbsI 1295 GTCTTC GAAGAC(2/6)
BcgI 2227 CGACATTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 425 GTATCC GTATCC(6/5)
BfaI 1747 CTAG C^TAG
BlpI 624 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 624 GCTGAGC GC^T[ACGT]AGC
BpuAI 1295 GTCTTC GAAGAC(2/6)
BsaAI 2754 CACGTA [CT]AC^GT[GA]
BsmBI 1018 GAGACG CGTCTC(1/5)
BsmI 2250 GAATGC GAATGC(1/-1)
BspLU11I 1152 ACATGT A^CATGT
BsrDI 1245 CATTGC GCAATG(2/0)
BssSI 496 CTCGTG CACGAG(-5/-1)
BstUI 1064 CGCG CG^CG
CelII 624 GCTGAGC GC^T[ACGT]AGC
DraIII 2924 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
FspI 1626 TGCGCA TGC^GCA
HgaI 1020 GACGC GACGC(5/10)
HincII 829 GTCAAC GT[CT]^[GA]AC
HindII 829 GTCAAC GT[CT]^[GA]AC
KasI 2681 GGCGCC G^GCGCC
KpnI 338 GGTACC GGTAC^C
MaeI 1747 CTAG C^TAG
MvnI 1064 CGCG CG^CG
NarI 2681 GGCGCC GG^CGCC
NruI 1063 TCGCGA TCG^CGA
NspI 1152 ACATGT [GA]CATG^[CT]
PinAI 1301 ACCGGT A^CCGGT
SexAI 1607 ACCTGGT A^CC[AT]GGT
SfoI 2681 GGCGCC GGC^GCC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AccI 2458 GTCTAC GT^[AC][GT]AC
2944 GTCTAC
AcsI 2064 AAATTT [GA]^AATT[CT]
2287 AAATTT
AcyI 2455 GACGTC G[GA]^CG[CT]C
2681 GGCGCC
ApoI 2064 AAATTT [GA]^AATT[CT]
2287 AAATTT
BglI 2086 GCCATTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2604 GCCCCCCAGGC
BglII 683 AGATCT A^GATCT
2243 AGATCT
BsaHI 2455 GACGTC G[GA]^CG[CT]C
2681 GGCGCC
BsaI 409 GAGACC GGTCTC(1/5)
1804 GAGACC
BsrBI 2380 GAGCGG CCGCTC(-3/-3)
2698 GAGCGG
BsrFI 881 ACCGGC [GA]^CCGG[CT]
1301 ACCGGT
BstXI 31 CCACCATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1475 CCAAGGCTGTGG
Cfr10I 881 ACCGGC [GA]^CCGG[CT]
1301 ACCGGT
DrdI 1088 GACAGCTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1558 GACTGGTGTGTC
Hin1I 2455 GACGTC G[GA]^CG[CT]C
2681 GGCGCC
HindIII 776 AAGCTT A^AGCTT
3088 AAGCTT
MaeII 2456 ACGT A^CGT
2755 ACGT
MseI 218 TTAA T^TAA
847 TTAA
PflMI 1371 CCACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1452 CCAGGAAATGG
SapI 2584 GAAGAGC GCTCTTC(1/4)
2254 GCTCTTC
SmaI 1313 CCCGGG CCC^GGG
2711 CCCGGG
SmlI 1227 CTTGAG C^T[CT][GA]AG
2939 CTCAAG
Tru9I 218 TTAA T^TAA
847 TTAA
Van91I 1371 CCACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1452 CCAGGAAATGG
XmaCI 1313 CCCGGG C^CCGGG
2711 CCCGGG
XmaI 1313 CCCGGG C^CCGGG
2711 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI ApaI AspHI AvaI AvaII
BalI BamHI BanI BanII BbvCI
BbvI BcnI BmyI BpmI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstAPI BstF5I
BstNI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI StuI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII