Restriction map for
pF1KA1910
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# NO CUT ENZYMES ( 95 enzymes) #
##################################
Acc65I AccIII AflII AgeI Alw44I
Aor51HI ApaLI AscI Asp718I AspI
AviII AvrII BalI BbrPI BbsI
BciVI BclI BfrI BlnI BlpI
Bpu1102I BpuAI BsaWI BseAI BsiWI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrGI BssHII Bst1107I BstAPI
BstEII BstPI BstZ17I CelII ClaI
CpoI DrdI Eco47III EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII KpnI KspI MluI MluNI
MroI MscI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Tth111I Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AatII 27 GACGTC GACGT^C
AccI 118 GTAGAC GT^[AC][GT]AC
AclI 52 AACGTT AA^CGTT
AcsI 1324 AAATTC [GA]^AATT[CT]
AhdI 1306 GACACGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 902 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 2045 GGGCCC GGGCC^C
ApoI 1324 AAATTC [GA]^AATT[CT]
AseI 1981 ATTAAT AT^TAAT
AsnI 1981 ATTAAT AT^TAAT
AspEI 1306 GACACGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1421 GGATCC G^GATCC
BanI 816 GGCGCC G^G[CT][GA]CC
BanII 2045 GGGCCC G[GA]GC[CT]^C
BbeI 816 GGCGCC GGCGC^C
BcgI 712 CGAGTGGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1022 GCCCTGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 115 CACGTA [CT]AC^GT[GA]
BsaBI 948 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 708 CGGCCG CG[GA][CT]^CG
BsmI 696 GAATGC GAATGC(1/-1)
BsrDI 92 GCAATG GCAATG(2/0)
BsrFI 1680 GCCGGT [GA]^CCGG[CT]
BssSI 1915 CTCGTG CACGAG(-5/-1)
BstBI 362 TTCGAA TT^CGAA
BstXI 934 CCAGGCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 817 GCGC GCG^C
Cfr10I 1680 GCCGGT [GA]^CCGG[CT]
EaeI 708 CGGCCG [CT]^GGCC[GA]
EagI 708 CGGCCG C^GGCCG
Eam1105I 1306 GACACGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 708 CGGCCG C^GGCCG
Eco52I 708 CGGCCG C^GGCCG
EcoNI 621 CCTGCTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 637 CCTTGG C^C[AT][AT]GG
FauI 1330 GCGGG CCCGC(4/6)
HaeII 816 GGCGCC [GA]GCGC^[CT]
HhaI 817 GCGC GCG^C
HinP1I 817 GCGC G^CGC
KasI 816 GGCGCC G^GCGCC
MamI 948 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1607 CAATTG C^AATTG
MunI 1607 CAATTG C^AATTG
NarI 816 GGCGCC GG^CGCC
NspI 1910 GCATGC [GA]CATG^[CT]
NspV 362 TTCGAA TT^CGAA
PleI 2005 GACTC GAGTC(4/5)
PshAI 775 GACTTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1981 ATTAAT AT^TAAT
Psp1406I 52 AACGTT AA^CGTT
SfoI 816 GGCGCC GGC^GCC
SfuI 362 TTCGAA TT^CGAA
SgrAI 1679 CGCCGGTG C[GA]^CCGG[CT]G
SmlI 1666 CTCAAG C^T[CT][GA]AG
SphI 1910 GCATGC GCATG^C
StyI 637 CCTTGG C^C[AT][AT]GG
XcmI 1847 CCATCTCGGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
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AccII 1329 CGCG CG^CG
1971 CGCG
AocI 1272 CCTCAGG CC^T[ACGT]AGG
1926 CCTGAGG
Asp700I 286 GAAATCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
682 GAAAACATTC
AspHI 472 GTGCTC G[AT]GC[AT]^C
1596 GTGCTC
BbvCI 1735 CCTCAGC CCTCAGC(-5/-2)
1446 GCTGAGG
BglII 80 AGATCT A^GATCT
1727 AGATCT
BsiHKAI 472 GTGCTC G[AT]GC[AT]^C
1596 GTGCTC
BspMI 507 ACCTGC ACCTGC(4/8)
1442 ACCTGC
BstUI 1329 CGCG CG^CG
1971 CGCG
Bsu36I 1272 CCTCAGG CC^T[ACGT]AGG
1926 CCTGAGG
DraI 480 TTTAAA TTT^AAA
1062 TTTAAA
DraIII 1180 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
2050 CACAGAGTG
DsaI 1578 CCACGG C^C[GA][CT]GG
1901 CCGTGG
EarI 2007 CTCTTC CTCTTC(1/4)
832 GAAGAG
Eco81I 1272 CCTCAGG CC^T[ACGT]AGG
1926 CCTGAGG
HincII 1551 GTTAAC GT[CT]^[GA]AC
1764 GTTAAC
HindII 1551 GTTAAC GT[CT]^[GA]AC
1764 GTTAAC
HpaI 1551 GTTAAC GTT^AAC
1764 GTTAAC
Ksp632I 2007 CTCTTC CTCTTC(1/4)
832 GAAGAG
MvnI 1329 CGCG CG^CG
1971 CGCG
PpuMI 107 GGGACCT [GA]G^G[AT]CC[CT]
1450 AGGTCCC
SfaNI 1957 GATGC GCATC(5/9)
1166 GCATC
TfiI 12 GATTC G^A[AT]TC
802 GATTC
Tsp45I 1885 GTCAC ^GT[GC]AC
2003 GTGAC
XmnI 286 GAAATCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
682 GAAAACATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AcyI AfaI AflIII AluI
AlwI AvaI AvaII BbvI BcnI
BfaI BmyI BpmI BsaHI BsaI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraII
EcoO109I EcoRII Fnu4HI FokI HaeIII
HapII HgaI Hin1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfcI SmaI TaqI Tru9I TseI
Tsp509I TspRI TthHB8I XhoII XmaCI
XmaI