Restriction map for
pF1KA1936
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# NO CUT ENZYMES ( 105 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI Alw44I ApaLI AscI Asp700I
Asp718I AspI AviII BamHI BbeI
BbrPI BcgI BclI BfrI BglI
BlpI Bpu1102I BsaBI BsaWI BseAI
BsiEI BsiWI Bsp1407I BspDI BspEI
BspHI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
CelII Cfr10I ClaI CpoI EagI
EclXI Eco52I EcoO65I EcoRI EcoT22I
FauI FbaI FseI FspI HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SpeI SphI
Sse8387I SspBI SspI SwaI TaqI
Tth111I TthHB8I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AatII 60 GACGTC GACGT^C
AccII 2103 CGCG CG^CG
AhdI 996 GACAGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 657 AGCGCT AGC^GCT
ApaI 1834 GGGCCC GGGCC^C
AseI 1240 ATTAAT AT^TAAT
AsnI 1240 ATTAAT AT^TAAT
AspEI 996 GACAGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 2117 CCCGGG C^[CT]CG[GA]G
AvrII 1182 CCTAGG C^CTAGG
BanI 2045 GGTGCC G^G[CT][GA]CC
BbsI 1273 GTCTTC GAAGAC(2/6)
BciVI 408 GTATCC GTATCC(6/5)
BlnI 1182 CCTAGG C^CTAGG
BpmI 2353 CTCCAG CTGGAG(16/14)
BpuAI 1273 GTCTTC GAAGAC(2/6)
BsaAI 440 TACGTA [CT]AC^GT[GA]
BsmBI 367 GAGACG CGTCTC(1/5)
BsmI 1849 GCATTC GAATGC(1/-1)
BsoBI 2117 CCCGGG C^[CT]CG[GA]G
BsrBI 333 CCGCTC CCGCTC(-3/-3)
BsrDI 1047 CATTGC GCAATG(2/0)
BstAPI 1859 GCAACAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 2103 CGCG CG^CG
BstXI 1710 CCACTGCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 723 TTTAAA TTT^AAA
Eam1105I 996 GACAGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 657 AGCGCT AGC^GCT
EcoRV 103 GATATC GAT^ATC
HincII 1059 GTTGAC GT[CT]^[GA]AC
HindII 1059 GTTGAC GT[CT]^[GA]AC
MvnI 2103 CGCG CG^CG
NcoI 2329 CCATGG C^CATGG
PflMI 1222 CCAATTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 1240 ATTAAT AT^TAAT
SapI 2229 GCTCTTC GCTCTTC(1/4)
SexAI 1946 ACCTGGT A^CC[AT]GGT
SmaI 2117 CCCGGG CCC^GGG
SnaBI 440 TACGTA TAC^GTA
Van91I 1222 CCAATTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2207 CCAGTGTTGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 2117 CCCGGG C^CCGGG
XmaI 2117 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 232 AAATTT [GA]^AATT[CT]
1318 AAATTC
AfaI 270 GTAC GT^AC
2269 GTAC
AlwI 718 GATCC GGATC(4/5)
3 GGATC
ApoI 232 AAATTT [GA]^AATT[CT]
1318 AAATTC
BalI 635 TGGCCA TGG^CCA
911 TGGCCA
BglII 1912 AGATCT A^GATCT
2246 AGATCT
BspLU11I 311 ACATGT A^CATGT
1484 ACATGT
BspMI 1569 GCAGGT ACCTGC(4/8)
1977 GCAGGT
DraIII 1028 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
2083 CACGCCGTG
DrdI 856 GACACCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1942 GACCACCTGGTC
EaeI 635 TGGCCA [CT]^GGCC[GA]
911 TGGCCA
EcoT14I 1182 CCTAGG C^C[AT][AT]GG
2329 CCATGG
HaeII 657 AGCGCT [GA]GCGC^[CT]
1872 AGCGCC
MluNI 635 TGGCCA TGG^CCA
911 TGGCCA
MscI 635 TGGCCA TGG^CCA
911 TGGCCA
MslI 1635 CACCAGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1711 CACTGCTATG
MspA1I 1681 CAGCGG C[AC]G^C[GT]G
2032 CAGCGG
NspI 311 ACATGT [GA]CATG^[CT]
1484 ACATGT
RsaI 270 GTAC GT^AC
2269 GTAC
SmlI 96 CTTGAG C^T[CT][GA]AG
646 CTTGAG
StuI 295 AGGCCT AGG^CCT
974 AGGCCT
StyI 1182 CCTAGG C^C[AT][AT]GG
2329 CCATGG
TfiI 1704 GATTC G^A[AT]TC
1924 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AcyI AflIII AluI AlwNI
AocI AspHI AvaII BanII BbvCI
BbvI BcnI BfaI BmyI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EarI Eco81I
EcoNI EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PstI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII