Restriction map for
pF1KA1953
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# NO CUT ENZYMES ( 74 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
Aor51HI AscI AseI AsnI Asp700I
Asp718I AspI AvrII BamHI BcgI
BciVI BfaI BfrI BlnI BseAI
BsiWI BspEI BspHI BspLU11I BssHII
Bst1107I BstBI BstZ17I CpoI EagI
EclXI Eco47III Eco52I EcoT22I FseI
HincII HindII HindIII HpaI KpnI
KspI MaeI MfeI MluI MroI
MunI NheI NotI NruI NsiI
NspV PacI PflFI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
SwaI Tth111I XbaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 787 GACGTC GACGT^C
AccI 171 GTCTAC GT^[AC][GT]AC
AgeI 1938 ACCGGT A^CCGGT
AhdI 95 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 95 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2222 TGCGCA TGC^GCA
BbrPI 1579 CACGTG CAC^GTG
BbvCI 1866 GCTGAGG CCTCAGC(-5/-2)
BclI 2133 TGATCA T^GATCA
BglI 1635 GCCTGTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2257 AGATCT A^GATCT
BsaBI 2035 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 1229 CGGTCG CG[GA][CT]^CG
BsmI 215 GAATGC GAATGC(1/-1)
Bsp1407I 1079 TGTACA T^GTACA
BspDI 1462 ATCGAT AT^CGAT
BspMI 1574 ACCTGC ACCTGC(4/8)
BsrGI 1079 TGTACA T^GTACA
BssSI 1384 CACGAG CACGAG(-5/-1)
BstAPI 1947 GCAGAGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1587 GGTCACC G^GT[ACGT]ACC
BstPI 1587 GGTCACC G^GT[ACGT]ACC
ClaI 1462 ATCGAT AT^CGAT
DraI 715 TTTAAA TTT^AAA
DraIII 2079 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 95 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1587 GGTCACC G^GT[ACGT]ACC
EcoRI 157 GAATTC G^AATTC
EcoRV 1041 GATATC GAT^ATC
FbaI 2133 TGATCA T^GATCA
FspI 2222 TGCGCA TGC^GCA
MamI 2035 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 2445 GCCGGC GCC^GGC
NcoI 1925 CCATGG C^CATGG
NdeI 1090 CATATG CA^TATG
NgoMIV 2445 GCCGGC G^CCGGC
NspI 1913 GCATGT [GA]CATG^[CT]
PaeR7I 2266 CTCGAG C^TCGAG
PinAI 1938 ACCGGT A^CCGGT
PmaCI 1579 CACGTG CAC^GTG
PmlI 1579 CACGTG CAC^GTG
SapI 2695 GAAGAGC GCTCTTC(1/4)
SbfI 2566 CCTGCAGG CCTGCA^GG
SmaI 2107 CCCGGG CCC^GGG
Sse8387I 2566 CCTGCAGG CCTGCA^GG
SspBI 1079 TGTACA T^GTACA
SspI 417 AATATT AAT^ATT
StuI 1681 AGGCCT AGG^CCT
XhoI 2266 CTCGAG C^TCGAG
XmaCI 2107 CCCGGG C^CCGGG
XmaI 2107 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 34 enzymes) #
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Alw44I 841 GTGCAC G^TGCAC
1381 GTGCAC
AocI 2118 CCTCAGG CC^T[ACGT]AGG
2623 CCTGAGG
ApaLI 841 GTGCAC G^TGCAC
1381 GTGCAC
BalI 2196 TGGCCA TGG^CCA
2802 TGGCCA
BbeI 494 GGCGCC GGCGC^C
2448 GGCGCC
BlpI 552 GCTGAGC GC^T[ACGT]AGC
1755 GCTGAGC
Bpu1102I 552 GCTGAGC GC^T[ACGT]AGC
1755 GCTGAGC
BsaAI 1159 TACGTG [CT]AC^GT[GA]
1579 CACGTG
BsaI 1181 GAGACC GGTCTC(1/5)
2127 GGTCTC
BsmBI 1723 CGTCTC CGTCTC(1/5)
1237 GAGACG
BsrBI 1441 CCGCTC CCGCTC(-3/-3)
1795 GAGCGG
BsrDI 1539 CATTGC GCAATG(2/0)
2481 CATTGC
BstYI 1253 AGATCC [GA]^GATC[CT]
2257 AGATCT
Bsu36I 2118 CCTCAGG CC^T[ACGT]AGG
2623 CCTGAGG
CelII 552 GCTGAGC GC^T[ACGT]AGC
1755 GCTGAGC
DrdI 2143 GACTCTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2519 GACTGCTGGGTC
EaeI 2196 TGGCCA [CT]^GGCC[GA]
2802 TGGCCA
Eco81I 2118 CCTCAGG CC^T[ACGT]AGG
2623 CCTGAGG
EcoNI 1896 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2535 CCTGAATCAGG
EcoT14I 941 CCAAGG C^C[AT][AT]GG
1925 CCATGG
HaeII 494 GGCGCC [GA]GCGC^[CT]
2448 GGCGCC
KasI 494 GGCGCC G^GCGCC
2448 GGCGCC
MflI 1253 AGATCC [GA]^GATC[CT]
2257 AGATCT
MluNI 2196 TGGCCA TGG^CCA
2802 TGGCCA
MscI 2196 TGGCCA TGG^CCA
2802 TGGCCA
NarI 494 GGCGCC GG^CGCC
2448 GGCGCC
PflMI 1815 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2328 CCAGAGTGTGG
PvuII 269 CAGCTG CAG^CTG
538 CAGCTG
SacI 1758 GAGCTC GAGCT^C
2821 GAGCTC
SfoI 494 GGCGCC GGC^GCC
2448 GGCGCC
StyI 941 CCAAGG C^C[AT][AT]GG
1925 CCATGG
Van91I 1815 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2328 CCAGAGTGTGG
XcmI 819 CCAGGTTCTAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1167 CCACAGTGGCATTGG
XhoII 1253 AGATCC [GA]^GATC[CT]
2257 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII BanI BanII
BbsI BbvI BcnI BmyI BpmI
BpuAI BsaHI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EarI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I