Restriction map for
pF1KB0001
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# NO CUT ENZYMES ( 55 enzymes) #
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Acc65I AclI AflII AgeI AhdI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BfrI BglII BlnI
BsiWI BsmI BspDI BspLU11I Bst1107I
BstBI BstZ17I ClaI DraI Eam1105I
EcoRI EcoT22I FspI HindIII HpaI
KpnI MfeI MseI MunI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I SalI ScaI SfuI SpeI
SspI SwaI Tru9I XbaI XhoI
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# ONE CUT ENZYMES ( 24 enzymes) #
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AatII 5968 GACGTC GACGT^C
AcsI 5311 AAATTC [GA]^AATT[CT]
ApoI 5311 AAATTC [GA]^AATT[CT]
BbrPI 2251 CACGTG CAC^GTG
BciVI 7414 GTATCC GTATCC(6/5)
BclI 7208 TGATCA T^GATCA
BsaBI 3105 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 3197 TGTACA T^GTACA
BspHI 6470 TCATGA T^CATGA
BsrGI 3197 TGTACA T^GTACA
EcoRV 799 GATATC GAT^ATC
FbaI 7208 TGATCA T^GATCA
FseI 3738 GGCCGGCC GGCCGG^CC
KspI 3307 CCGCGG CCGC^GG
MamI 3105 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 7101 CATATG CA^TATG
PmaCI 2251 CACGTG CAC^GTG
PmlI 2251 CACGTG CAC^GTG
PvuI 6233 CGATCG CGAT^CG
RcaI 6470 TCATGA T^CATGA
SacII 3307 CCGCGG CCGC^GG
SgrAI 5788 CGCCGGTG C[GA]^CCGG[CT]G
SnaBI 7027 TACGTA TAC^GTA
SspBI 3197 TGTACA T^GTACA
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# TWO CUT ENZYMES ( 26 enzymes) #
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AccIII 5759 TCCGGA T^CCGGA
6551 TCCGGA
AflIII 4124 ACGCGT A^C[GA][CT]GT
7512 ACGCGT
Aor51HI 1101 AGCGCT AGC^GCT
1895 AGCGCT
Asp700I 1581 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4176 GAAGTCCTTC
BamHI 3891 GGATCC G^GATCC
5756 GGATCC
BsaAI 2251 CACGTG [CT]AC^GT[GA]
7027 TACGTA
BseAI 5759 TCCGGA T^CCGGA
6551 TCCGGA
BspEI 5759 TCCGGA T^CCGGA
6551 TCCGGA
BsrDI 5865 CATTGC GCAATG(2/0)
6080 GCAATG
BssHII 3390 GCGCGC G^CGCGC
7338 GCGCGC
BssSI 379 CTCGTG CACGAG(-5/-1)
5734 CTCGTG
CpoI 148 CGGTCCG CG^G[AT]CCG
2026 CGGACCG
DraIII 1500 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
2263 CACGCGGTG
EagI 836 CGGCCG C^GGCCG
2534 CGGCCG
EclXI 836 CGGCCG C^GGCCG
2534 CGGCCG
Eco47III 1101 AGCGCT AGC^GCT
1895 AGCGCT
Eco52I 836 CGGCCG C^GGCCG
2534 CGGCCG
MluI 4124 ACGCGT A^CGCGT
7512 ACGCGT
MroI 5759 TCCGGA T^CCGGA
6551 TCCGGA
RsrII 148 CGGTCCG CG^G[AT]CCG
2026 CGGACCG
SapI 2901 GCTCTTC GCTCTTC(1/4)
4197 GCTCTTC
SbfI 3399 CCTGCAGG CCTGCA^GG
3554 CCTGCAGG
SphI 1355 GCATGC GCATG^C
6601 GCATGC
Sse8387I 3399 CCTGCAGG CCTGCA^GG
3554 CCTGCAGG
TfiI 292 GATTC G^A[AT]TC
1552 GATTC
XmnI 1581 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4176 GAAGTCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 153 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI Alw44I AlwI AlwNI AocI
ApaI ApaLI AspHI AspI AvaI
AvaII BalI BanI BanII BbeI
BbsI BbvCI BbvI BcgI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DrdI
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflFI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SfiI SfoI SmaI SmlI StuI
StyI TaqI TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII XmaCI XmaI