Restriction map for
pF1KB0045
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# NO CUT ENZYMES ( 100 enzymes) #
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AatII AcyI AflII AgeI AhdI
Aor51HI ApaI AscI AseI AsnI
AspEI AviII AvrII BbeI BbrPI
BcgI BciVI BfrI BglII BlnI
BsaAI BsaHI BsaI BsiEI BsiWI
Bsp1407I BspDI BspHI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstEII
BstPI BstZ17I CfoI Cfr10I ClaI
CpoI DraII DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO109I EcoO65I FauI FseI FspI
HaeII HhaI Hin1I HinP1I HindIII
KasI KspI MluI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI PacI PaeR7I PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
PvuI RcaI RsrII SacII SalI
SbfI SexAI SfiI SfoI SgrAI
SmaI SnaBI SphI Sse8387I SspBI
SwaI XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 2218 GGTACC G^GTACC
AccI 1874 GTCTAC GT^[AC][GT]AC
AccII 53 CGCG CG^CG
AccIII 47 TCCGGA T^CCGGA
AclI 638 AACGTT AA^CGTT
Alw44I 890 GTGCAC G^TGCAC
AocI 2222 CCTTAGG CC^T[ACGT]AGG
ApaLI 890 GTGCAC G^TGCAC
Asp700I 710 GAACACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2218 GGTACC G^GTACC
BalI 232 TGGCCA TGG^CCA
BamHI 3775 GGATCC G^GATCC
BclI 3417 TGATCA T^GATCA
BcnI 1240 CCCGG CC^[GC]GG
BglI 153 GCCAGGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 47 TCCGGA [AT]^CCGG[AT]
BseAI 47 TCCGGA T^CCGGA
BspEI 47 TCCGGA T^CCGGA
BsrDI 608 CATTGC GCAATG(2/0)
BstBI 2556 TTCGAA TT^CGAA
BstUI 53 CGCG CG^CG
Bsu36I 2222 CCTTAGG CC^T[ACGT]AGG
EaeI 232 TGGCCA [CT]^GGCC[GA]
Eco81I 2222 CCTTAGG CC^T[ACGT]AGG
EcoRV 1036 GATATC GAT^ATC
EcoT14I 3021 CCTTGG C^C[AT][AT]GG
EcoT22I 1905 ATGCAT ATGCA^T
FbaI 3417 TGATCA T^GATCA
HpaI 375 GTTAAC GTT^AAC
KpnI 2218 GGTACC GGTAC^C
MfeI 470 CAATTG C^AATTG
MluNI 232 TGGCCA TGG^CCA
MroI 47 TCCGGA T^CCGGA
MscI 232 TGGCCA TGG^CCA
MunI 470 CAATTG C^AATTG
MvnI 53 CGCG CG^CG
NciI 1240 CCCGG CC^[GC]GG
NsiI 1905 ATGCAT ATGCA^T
NspV 2556 TTCGAA TT^CGAA
Psp1406I 638 AACGTT AA^CGTT
SapI 3247 GAAGAGC GCTCTTC(1/4)
SfuI 2556 TTCGAA TT^CGAA
StuI 382 AGGCCT AGG^CCT
StyI 3021 CCTTGG C^C[AT][AT]GG
XcmI 3530 CCAATCCACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 710 GAACACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AflIII 1870 ACATGT A^C[GA][CT]GT
3064 ACATGT
AspI 3857 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3963 GACAGTGTC
BbvCI 3803 CCTCAGC CCTCAGC(-5/-2)
4151 CCTCAGC
BlpI 877 GCTAAGC GC^T[ACGT]AGC
1753 GCTCAGC
Bpu1102I 877 GCTAAGC GC^T[ACGT]AGC
1753 GCTCAGC
BsaBI 1032 GATAGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3899 GATCTATATC
BsmBI 1511 CGTCTC CGTCTC(1/5)
1347 GAGACG
BspLU11I 1870 ACATGT A^CATGT
3064 ACATGT
BstXI 613 CCACCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2480 CCAAATGTTTGG
CelII 877 GCTAAGC GC^T[ACGT]AGC
1753 GCTCAGC
EcoNI 492 CCTTGTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2386 CCTTCTGGAGG
HgaI 51 GACGC GACGC(5/10)
4289 GACGC
HincII 375 GTTAAC GT[CT]^[GA]AC
3260 GTCAAC
HindII 375 GTTAAC GT[CT]^[GA]AC
3260 GTCAAC
MaeII 639 ACGT A^CGT
3131 ACGT
MamI 1032 GATAGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3899 GATCTATATC
PflFI 3857 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3963 GACAGTGTC
SacI 276 GAGCTC GAGCT^C
3395 GAGCTC
SmlI 1514 CTCAAG C^T[CT][GA]AG
3638 CTCAAG
SpeI 346 ACTAGT A^CTAGT
3403 ACTAGT
TaqI 1134 TCGA T^CGA
2557 TCGA
Tth111I 3857 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3963 GACAGTGTC
TthHB8I 1134 TCGA T^CGA
2557 TCGA
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# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaI AvaII
BanI BanII BbsI BbvI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI EarI EcoRI EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PflMI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SspI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XhoII