Restriction map for
pF1KB0048
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# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AflIII AgeI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspI AviII AvrII BbeI BbrPI
BbvCI BfrI BglI BlnI BsaHI
BsiEI BsiWI BspLU11I BssHII BstEII
BstPI BstXI Bsu36I CpoI DraI
DrdI EagI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRV FseI FspI
HaeII Hin1I KasI KpnI MluI
NaeI NarI NdeI NgoMIV NotI
NruI PacI PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SacI SalI
SbfI SexAI SfiI SfoI SgrAI
SmaI SpeI Sse8387I SwaI Tth111I
XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccIII 977 TCCGGA T^CCGGA
ApaI 3508 GGGCCC GGGCC^C
BalI 4862 TGGCCA TGG^CCA
BanI 3408 GGCACC G^G[CT][GA]CC
BglII 3787 AGATCT A^GATCT
BsaAI 3097 TACGTA [CT]AC^GT[GA]
BseAI 977 TCCGGA T^CCGGA
BsmI 2624 GAATGC GAATGC(1/-1)
BspDI 4538 ATCGAT AT^CGAT
BspEI 977 TCCGGA T^CCGGA
BspMI 3595 GCAGGT ACCTGC(4/8)
BsrBI 1829 CCGCTC CCGCTC(-3/-3)
BsrFI 896 GCCGGT [GA]^CCGG[CT]
BssSI 1991 CACGAG CACGAG(-5/-1)
Bst1107I 1725 GTATAC GTA^TAC
BstAPI 4081 GCACACATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 1725 GTATAC GTA^TAC
Cfr10I 896 GCCGGT [GA]^CCGG[CT]
ClaI 4538 ATCGAT AT^CGAT
EcoNI 3499 CCTTCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 4362 GAATTC G^AATTC
EcoT22I 2626 ATGCAT ATGCA^T
HindIII 2735 AAGCTT A^AGCTT
KspI 469 CCGCGG CCGC^GG
MfeI 2132 CAATTG C^AATTG
MluNI 4862 TGGCCA TGG^CCA
MroI 977 TCCGGA T^CCGGA
MscI 4862 TGGCCA TGG^CCA
MunI 2132 CAATTG C^AATTG
NheI 1710 GCTAGC G^CTAGC
NsiI 2626 ATGCAT ATGCA^T
PaeR7I 3124 CTCGAG C^TCGAG
SacII 469 CCGCGG CCGC^GG
ScaI 2896 AGTACT AGT^ACT
SnaBI 3097 TACGTA TAC^GTA
SphI 1035 GCATGC GCATG^C
XhoI 3124 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Asp700I 2326 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3903 GAATCAATTC
AspHI 3869 GTGCTC G[AT]GC[AT]^C
4322 GAGCAC
BamHI 2979 GGATCC G^GATCC
3938 GGATCC
BcgI 2409 GCATGATGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3116 GCAGAGACCTCG
BciVI 1602 GGATAC GTATCC(6/5)
2127 GTATCC
BlpI 1702 GCTGAGC GC^T[ACGT]AGC
1831 GCTCAGC
Bpu1102I 1702 GCTGAGC GC^T[ACGT]AGC
1831 GCTCAGC
BsgI 4079 CTGCAC GTGCAG(16/14)
4634 CTGCAC
BsiHKAI 3869 GTGCTC G[AT]GC[AT]^C
4322 GAGCAC
BspHI 1200 TCATGA T^CATGA
2631 TCATGA
BstBI 1643 TTCGAA TT^CGAA
1805 TTCGAA
CelII 1702 GCTGAGC GC^T[ACGT]AGC
1831 GCTCAGC
DraII 2515 GGGGCCT [GA]G^G[ACGT]CC[CT]
3507 AGGGCCC
DraIII 4325 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
5111 CACACTGTG
EcoO109I 2515 GGGGCCT [GA]G^G[ACGT]CC[CT]
3507 AGGGCCC
HincII 196 GTTAAC GT[CT]^[GA]AC
403 GTTAAC
HindII 196 GTTAAC GT[CT]^[GA]AC
403 GTTAAC
HpaI 196 GTTAAC GTT^AAC
403 GTTAAC
NcoI 3615 CCATGG C^CATGG
5070 CCATGG
NspV 1643 TTCGAA TT^CGAA
1805 TTCGAA
RcaI 1200 TCATGA T^CATGA
2631 TCATGA
SapI 3361 GAAGAGC GCTCTTC(1/4)
3436 GAAGAGC
SfuI 1643 TTCGAA TT^CGAA
1805 TTCGAA
StuI 880 AGGCCT AGG^CCT
1897 AGGCCT
XbaI 1314 TCTAGA T^CTAGA
3589 TCTAGA
XmnI 2326 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3903 GAATCAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AhdI AluI AlwI AlwNI ApoI
AspEI AvaI AvaII BanII BbsI
BbvI BclI BcnI BfaI BmyI
BpmI BpuAI BsaBI BsaI BsaJI
BsaWI BseRI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I Bsp1407I
BsrDI BsrGI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EaeI
Eam1105I EarI EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII