Restriction map for
pF1KB1311
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# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII AgeI AhdI Alw44I ApaLI
AscI AseI AsnI AspEI AviII
AvrII BclI BglII BlnI BsiWI
Bsp1407I BspDI BspHI BsrDI BsrGI
Bst1107I BstBI BstZ17I ClaI CpoI
DraI DrdI Eam1105I EcoNI EcoRI
FbaI FseI FspI HpaI MfeI
MluI MunI NdeI NotI NruI
NspV PacI PinAI PmeI PshBI
RcaI RsrII SacI SalI SbfI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI XbaI
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# ONE CUT ENZYMES ( 26 enzymes) #
###################################
AccI 3226 GTCTAC GT^[AC][GT]AC
AccIII 1427 TCCGGA T^CCGGA
AclI 3232 AACGTT AA^CGTT
AflII 2764 CTTAAG C^TTAAG
ApaI 2481 GGGCCC GGGCC^C
Asp700I 3459 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BciVI 2280 GGATAC GTATCC(6/5)
BfrI 2764 CTTAAG C^TTAAG
BseAI 1427 TCCGGA T^CCGGA
BsmBI 2769 GAGACG CGTCTC(1/5)
BspEI 1427 TCCGGA T^CCGGA
BssHII 427 GCGCGC G^CGCGC
BstAPI 789 GCACTATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2988 CCACGATATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EagI 1278 CGGCCG C^GGCCG
EclXI 1278 CGGCCG C^GGCCG
Eco52I 1278 CGGCCG C^GGCCG
HindIII 1288 AAGCTT A^AGCTT
MroI 1427 TCCGGA T^CCGGA
NheI 3702 GCTAGC G^CTAGC
PshAI 3570 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3232 AACGTT AA^CGTT
PvuI 2340 CGATCG CGAT^CG
ScaI 1949 AGTACT AGT^ACT
SfiI 3191 GGCCTGGTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
XmnI 3459 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AocI 480 CCTGAGG CC^T[ACGT]AGG
2316 CCTGAGG
AspHI 3328 GTGCTC G[AT]GC[AT]^C
3904 GTGCTC
AspI 283 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
737 GACCAGGTC
BalI 254 TGGCCA TGG^CCA
3420 TGGCCA
BbrPI 1225 CACGTG CAC^GTG
2943 CACGTG
BfaI 2467 CTAG C^TAG
3703 CTAG
BglI 491 GCCTCTATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3192 GCCTGGTCGGC
BsaBI 199 GATCCGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
900 GATGTTCATC
BsiHKAI 3328 GTGCTC G[AT]GC[AT]^C
3904 GTGCTC
BsmI 1956 GAATGC GAATGC(1/-1)
1504 GCATTC
BspLU11I 1609 ACATGT A^CATGT
1640 ACATGT
BstEII 182 GGTCACC G^GT[ACGT]ACC
3211 GGTTACC
BstPI 182 GGTCACC G^GT[ACGT]ACC
3211 GGTTACC
Bsu36I 480 CCTGAGG CC^T[ACGT]AGG
2316 CCTGAGG
Eco81I 480 CCTGAGG CC^T[ACGT]AGG
2316 CCTGAGG
EcoO65I 182 GGTCACC G^GT[ACGT]ACC
3211 GGTTACC
EcoRV 1846 GATATC GAT^ATC
3362 GATATC
EcoT22I 4 ATGCAT ATGCA^T
1958 ATGCAT
KspI 35 CCGCGG CCGC^GG
960 CCGCGG
MaeI 2467 CTAG C^TAG
3703 CTAG
MamI 199 GATCCGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
900 GATGTTCATC
MluNI 254 TGGCCA TGG^CCA
3420 TGGCCA
MscI 254 TGGCCA TGG^CCA
3420 TGGCCA
NaeI 1571 GCCGGC GCC^GGC
3086 GCCGGC
NgoMIV 1571 GCCGGC G^CCGGC
3086 GCCGGC
NsiI 4 ATGCAT ATGCA^T
1958 ATGCAT
PaeR7I 673 CTCGAG C^TCGAG
1901 CTCGAG
PflFI 283 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
737 GACCAGGTC
PmaCI 1225 CACGTG CAC^GTG
2943 CACGTG
PmlI 1225 CACGTG CAC^GTG
2943 CACGTG
SacII 35 CCGCGG CCGC^GG
960 CCGCGG
SapI 15 GCTCTTC GCTCTTC(1/4)
3906 GCTCTTC
SexAI 738 ACCAGGT A^CC[AT]GGT
2627 ACCTGGT
StuI 2320 AGGCCT AGG^CCT
3542 AGGCCT
Tth111I 283 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
737 GACCAGGTC
XhoI 673 CTCGAG C^TCGAG
1901 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
Acc65I AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
Aor51HI ApoI Asp718I AvaI AvaII
BamHI BanI BanII BbeI BbsI
BbvCI BbvI BcgI BcnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BssSI BstF5I
BstNI BstUI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
Eco47III EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KasI KpnI Ksp632I MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI