Restriction map for
pF1KB1434
##################################
# NO CUT ENZYMES ( 47 enzymes) #
##################################
AclI AflII AgeI AscI AvrII
BciVI BfrI BlnI Bsp1407I BspDI
BspLU11I BsrGI BssHII ClaI DraI
EagI EclXI Eco52I EcoRI EcoRV
EcoT22I FseI HpaI KspI MfeI
MluI MunI NdeI NheI NotI
NsiI PacI PaeR7I PinAI PmeI
Psp1406I PvuI SacII SalI SfiI
SgrAI SnaBI SpeI SspBI SspI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 1407 GACGTC GACGT^C
Acc65I 745 GGTACC G^GTACC
AccI 2068 GTATAC GT^[AC][GT]AC
Alw44I 1498 GTGCAC G^TGCAC
ApaLI 1498 GTGCAC G^TGCAC
AseI 2128 ATTAAT AT^TAAT
AsnI 2128 ATTAAT AT^TAAT
Asp718I 745 GGTACC G^GTACC
AviII 3434 TGCGCA TGC^GCA
BbeI 4836 GGCGCC GGCGC^C
BbrPI 4024 CACGTG CAC^GTG
BclI 6005 TGATCA T^GATCA
BglI 2269 GCCCTAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 135 GAGACC GGTCTC(1/5)
BsiEI 6098 CGGTCG CG[GA][CT]^CG
BsiWI 3137 CGTACG C^GTACG
BssSI 1224 CACGAG CACGAG(-5/-1)
Bst1107I 2068 GTATAC GTA^TAC
BstBI 677 TTCGAA TT^CGAA
BstZ17I 2068 GTATAC GTA^TAC
CpoI 2524 CGGTCCG CG^G[AT]CCG
FbaI 6005 TGATCA T^GATCA
FspI 3434 TGCGCA TGC^GCA
HindIII 163 AAGCTT A^AGCTT
KasI 4836 GGCGCC G^GCGCC
KpnI 745 GGTACC GGTAC^C
NaeI 5564 GCCGGC GCC^GGC
NarI 4836 GGCGCC GG^CGCC
NgoMIV 5564 GCCGGC G^CCGGC
NspV 677 TTCGAA TT^CGAA
PmaCI 4024 CACGTG CAC^GTG
PmlI 4024 CACGTG CAC^GTG
PshAI 3544 GACCGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2128 ATTAAT AT^TAAT
RsrII 2524 CGGTCCG CG^G[AT]CCG
SbfI 3237 CCTGCAGG CCTGCA^GG
SfoI 4836 GGCGCC GGC^GCC
SfuI 677 TTCGAA TT^CGAA
Sse8387I 3237 CCTGCAGG CCTGCA^GG
XbaI 5088 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccIII 3656 TCCGGA T^CCGGA
4938 TCCGGA
AflIII 3134 ACACGT A^C[GA][CT]GT
5228 ACGTGT
AhdI 729 GACTGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5362 GACTACAAGTC
AocI 4980 CCTCAGG CC^T[ACGT]AGG
5338 CCTGAGG
Aor51HI 3064 AGCGCT AGC^GCT
3974 AGCGCT
ApaI 2262 GGGCCC GGGCC^C
4039 GGGCCC
AspEI 729 GACTGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5362 GACTACAAGTC
BamHI 435 GGATCC G^GATCC
1268 GGATCC
BbvCI 3556 CCTCAGC CCTCAGC(-5/-2)
6246 CCTCAGC
BsaAI 3135 CACGTA [CT]AC^GT[GA]
4024 CACGTG
BsaBI 561 GATAGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2725 GATCCTGATC
BseAI 3656 TCCGGA T^CCGGA
4938 TCCGGA
BsmI 1311 GAATGC GAATGC(1/-1)
1629 GCATTC
BspEI 3656 TCCGGA T^CCGGA
4938 TCCGGA
BstEII 2565 GGTCACC G^GT[ACGT]ACC
4779 GGTCACC
BstPI 2565 GGTCACC G^GT[ACGT]ACC
4779 GGTCACC
Bsu36I 4980 CCTCAGG CC^T[ACGT]AGG
5338 CCTGAGG
Eam1105I 729 GACTGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5362 GACTACAAGTC
Eco47III 3064 AGCGCT AGC^GCT
3974 AGCGCT
Eco81I 4980 CCTCAGG CC^T[ACGT]AGG
5338 CCTGAGG
EcoNI 3192 CCTCCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4177 CCTACTCAAGG
EcoO65I 2565 GGTCACC G^GT[ACGT]ACC
4779 GGTCACC
HincII 1638 GTCAAC GT[CT]^[GA]AC
3848 GTCAAC
HindII 1638 GTCAAC GT[CT]^[GA]AC
3848 GTCAAC
MamI 561 GATAGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2725 GATCCTGATC
MroI 3656 TCCGGA T^CCGGA
4938 TCCGGA
NcoI 192 CCATGG C^CATGG
3302 CCATGG
NruI 571 TCGCGA TCG^CGA
4122 TCGCGA
SexAI 1001 ACCTGGT A^CC[AT]GGT
2171 ACCTGGT
SmlI 1878 CTTGAG C^T[CT][GA]AG
4181 CTCAAG
SphI 806 GCATGC GCATG^C
4080 GCATGC
StuI 506 AGGCCT AGG^CCT
4857 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI Asp700I
AspHI AspI AvaI AvaII BalI
BanI BanII BbsI BbvI BcgI
BcnI BfaI BglII BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DrdI DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflFI PflMI PleI PpuMI
PstI PvuII RcaI RsaI SacI
SapI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmaI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII XmaCI XmaI XmnI