Restriction map for pF1KB3598
##################################
# NO CUT ENZYMES (   83 enzymes) #
##################################

AccI        AccIII      AclI        AflII       AgeI        
AhdI        Aor51HI     AscI        AseI        AsnI        
Asp700I     AspEI       BbeI        BbvCI       BfrI        
BsaBI       BseAI       BsiWI       BsmBI       Bsp1407I    
BspDI       BspEI       BspHI       BsrBI       BsrGI       
BssHII      Bst1107I    BstBI       BstXI       BstZ17I     
ClaI        CpoI        DraI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      EcoT22I     
FseI        HaeII       HgaI        HpaI        KasI        
Ksp632I     KspI        MamI        MluI        MroI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PflMI       PinAI       PmeI        PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SalI        
SapI        ScaI        SfoI        SfuI        SgrAI       
SnaBI       SpeI        SspBI       StuI        SwaI        
Van91I      XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AatII        1682  GACGTC               GACGT^C
AccII        1181  CGCG                 CG^CG
AcyI         1682  GACGTC               G[GA]^CG[CT]C
Alw44I       1070  GTGCAC               G^TGCAC
ApaLI        1070  GTGCAC               G^TGCAC
AspI         2207  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        3845  TGCGCA               TGC^GCA
AvrII        2793  CCTAGG               C^CTAGG
BcgI         1010  CGAGGGGAATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI         2793  CCTAGG               C^CTAGG
BlpI         1331  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1331  GCTCAGC              GC^T[ACGT]AGC
BsaHI        1682  GACGTC               G[GA]^CG[CT]C
BsaWI        4322  TCCGGT               [AT]^CCGG[AT]
BsiEI        2963  CGACCG               CG[GA][CT]^CG
BspLU11I     4003  ACATGT               A^CATGT
BsrDI        3158  GCAATG               GCAATG(2/0)
BsrFI         396  ACCGGC               [GA]^CCGG[CT]
BstAPI       1894  GCAGGAAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI        1181  CGCG                 CG^CG
CelII        1331  GCTCAGC              GC^T[ACGT]AGC
Cfr10I        396  ACCGGC               [GA]^CCGG[CT]
DsaI          992  CCATGG               C^C[GA][CT]GG
FspI         3845  TGCGCA               TGC^GCA
Hin1I        1682  GACGTC               G[GA]^CG[CT]C
HincII        503  GTCAAC               GT[CT]^[GA]AC
HindII        503  GTCAAC               GT[CT]^[GA]AC
HindIII      3898  AAGCTT               A^AGCTT
MfeI         3584  CAATTG               C^AATTG
MseI          145  TTAA                 T^TAA
MunI         3584  CAATTG               C^AATTG
MvnI         1181  CGCG                 CG^CG
NcoI          992  CCATGG               C^CATGG
PflFI        2207  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PstI         3224  CTGCAG               CTGCA^G
SbfI         3223  CCTGCAGG             CCTGCA^GG
Sse8387I     3223  CCTGCAGG             CCTGCA^GG
SspI         4312  AATATT               AAT^ATT
Tru9I         145  TTAA                 T^TAA
Tth111I      2207  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

Acc65I       1312  GGTACC               G^GTACC
             3166  GGTACC              
AflIII       3883  ACGTGT               A^C[GA][CT]GT
             4003  ACATGT              
Asp718I      1312  GGTACC               G^GTACC
             3166  GGTACC              
BalI          132  TGGCCA               TGG^CCA
             3678  TGGCCA              
BamHI        1674  GGATCC               G^GATCC
             2644  GGATCC              
BbrPI         758  CACGTG               CAC^GTG
             3882  CACGTG              
BciVI        1115  GGATAC               GTATCC(6/5)
             4228  GGATAC              
BclI         2079  TGATCA               T^GATCA
             3393  TGATCA              
BglII         179  AGATCT               A^GATCT
             4425  AGATCT              
BsgI          120  CTGCAC               GTGCAG(16/14)
              695  GTGCAG              
DraIII       1046  CACAGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1487  CACCCGGTG           
EcoNI        2056  CCTGGAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3943  CCTAACCAAGG         
EcoRI        1385  GAATTC               G^AATTC
             4462  GAATTC              
EcoRV        3703  GATATC               GAT^ATC
             4294  GATATC              
FbaI         2079  TGATCA               T^GATCA
             3393  TGATCA              
KpnI         1312  GGTACC               GGTAC^C
             3166  GGTACC              
MluNI         132  TGGCCA               TGG^CCA
             3678  TGGCCA              
MscI          132  TGGCCA               TGG^CCA
             3678  TGGCCA              
MslI         2848  CATGGGCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             3715  CACTATGATG          
PaeR7I       2459  CTCGAG               C^TCGAG
             2504  CTCGAG              
PleI         2177  GACTC                GAGTC(4/5)
              615  GAGTC               
PmaCI         758  CACGTG               CAC^GTG
             3882  CACGTG              
PmlI          758  CACGTG               CAC^GTG
             3882  CACGTG              
PshAI         530  GACATCCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             3412  GACAGAGGTC          
XhoI         2459  CTCGAG               C^TCGAG
             2504  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  110 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       AocI        ApaI        ApoI        AspHI       
AvaI        AvaII       BanI        BanII       BbsI        
BbvI        BcnI        BfaI        BglI        BmyI        
BpmI        BpuAI       BsaAI       BsaI        BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        BsoBI       Bsp1286I    BspMI       
BsrI        BssKI       BssSI       BstEII      BstF5I      
BstNI       BstPI       BstYI       Bsu36I      Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DrdI        EaeI        Eco81I      EcoO109I    EcoO65I     
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PpuMI       
PvuII       RsaI        SacI        Sau3AI      Sau96I      
ScrFI       SexAI       SfaNI       SfcI        SfiI        
SmaI        SmlI        SphI        StyI        TaqI        
TfiI        TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XcmI        XhoII       XmaCI       XmaI        
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