################################## # NO CUT ENZYMES ( 83 enzymes) # ################################## AccI AccIII AclI AflII AgeI AhdI Aor51HI AscI AseI AsnI Asp700I AspEI BbeI BbvCI BfrI BsaBI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BsrBI BsrGI BssHII Bst1107I BstBI BstXI BstZ17I ClaI CpoI DraI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoT22I FseI HaeII HgaI HpaI KasI Ksp632I KspI MamI MluI MroI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflMI PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI ScaI SfoI SfuI SgrAI SnaBI SpeI SspBI StuI SwaI Van91I XbaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AatII 1682 GACGTC GACGT^C AccII 1181 CGCG CG^CG AcyI 1682 GACGTC G[GA]^CG[CT]C Alw44I 1070 GTGCAC G^TGCAC ApaLI 1070 GTGCAC G^TGCAC AspI 2207 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 3845 TGCGCA TGC^GCA AvrII 2793 CCTAGG C^CTAGG BcgI 1010 CGAGGGGAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlnI 2793 CCTAGG C^CTAGG BlpI 1331 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1331 GCTCAGC GC^T[ACGT]AGC BsaHI 1682 GACGTC G[GA]^CG[CT]C BsaWI 4322 TCCGGT [AT]^CCGG[AT] BsiEI 2963 CGACCG CG[GA][CT]^CG BspLU11I 4003 ACATGT A^CATGT BsrDI 3158 GCAATG GCAATG(2/0) BsrFI 396 ACCGGC [GA]^CCGG[CT] BstAPI 1894 GCAGGAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 1181 CGCG CG^CG CelII 1331 GCTCAGC GC^T[ACGT]AGC Cfr10I 396 ACCGGC [GA]^CCGG[CT] DsaI 992 CCATGG C^C[GA][CT]GG FspI 3845 TGCGCA TGC^GCA Hin1I 1682 GACGTC G[GA]^CG[CT]C HincII 503 GTCAAC GT[CT]^[GA]AC HindII 503 GTCAAC GT[CT]^[GA]AC HindIII 3898 AAGCTT A^AGCTT MfeI 3584 CAATTG C^AATTG MseI 145 TTAA T^TAA MunI 3584 CAATTG C^AATTG MvnI 1181 CGCG CG^CG NcoI 992 CCATGG C^CATGG PflFI 2207 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 3224 CTGCAG CTGCA^G SbfI 3223 CCTGCAGG CCTGCA^GG Sse8387I 3223 CCTGCAGG CCTGCA^GG SspI 4312 AATATT AAT^ATT Tru9I 145 TTAA T^TAA Tth111I 2207 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### Acc65I 1312 GGTACC G^GTACC 3166 GGTACC AflIII 3883 ACGTGT A^C[GA][CT]GT 4003 ACATGT Asp718I 1312 GGTACC G^GTACC 3166 GGTACC BalI 132 TGGCCA TGG^CCA 3678 TGGCCA BamHI 1674 GGATCC G^GATCC 2644 GGATCC BbrPI 758 CACGTG CAC^GTG 3882 CACGTG BciVI 1115 GGATAC GTATCC(6/5) 4228 GGATAC BclI 2079 TGATCA T^GATCA 3393 TGATCA BglII 179 AGATCT A^GATCT 4425 AGATCT BsgI 120 CTGCAC GTGCAG(16/14) 695 GTGCAG DraIII 1046 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG 1487 CACCCGGTG EcoNI 2056 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3943 CCTAACCAAGG EcoRI 1385 GAATTC G^AATTC 4462 GAATTC EcoRV 3703 GATATC GAT^ATC 4294 GATATC FbaI 2079 TGATCA T^GATCA 3393 TGATCA KpnI 1312 GGTACC GGTAC^C 3166 GGTACC MluNI 132 TGGCCA TGG^CCA 3678 TGGCCA MscI 132 TGGCCA TGG^CCA 3678 TGGCCA MslI 2848 CATGGGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 3715 CACTATGATG PaeR7I 2459 CTCGAG C^TCGAG 2504 CTCGAG PleI 2177 GACTC GAGTC(4/5) 615 GAGTC PmaCI 758 CACGTG CAC^GTG 3882 CACGTG PmlI 758 CACGTG CAC^GTG 3882 CACGTG PshAI 530 GACATCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3412 GACAGAGGTC XhoI 2459 CTCGAG C^TCGAG 2504 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 110 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI AocI ApaI ApoI AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaAI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrI BssKI BssSI BstEII BstF5I BstNI BstPI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DrdI EaeI Eco81I EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PpuMI PvuII RsaI SacI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SfiI SmaI SmlI SphI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmaCI XmaI