Restriction map for
pF1KB3816
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# NO CUT ENZYMES ( 43 enzymes) #
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AccIII AflII AgeI Alw44I ApaLI
AscI AvrII BbeI BfrI BglI
BlnI BsaBI BseAI BsiWI BspEI
BspLU11I BssHII Bst1107I BstBI BstZ17I
CpoI HpaI KasI MamI MroI
NarI NdeI NheI NotI NspV
PacI PinAI PmeI PshAI PvuI
RsrII SfiI SfoI SfuI SgrAI
SnaBI SpeI SwaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 2323 GACGTC GACGT^C
AclI 4298 AACGTT AA^CGTT
AcyI 2323 GACGTC G[GA]^CG[CT]C
Aor51HI 741 AGCGCT AGC^GCT
AseI 2602 ATTAAT AT^TAAT
AsnI 2602 ATTAAT AT^TAAT
AviII 1160 TGCGCA TGC^GCA
BamHI 212 GGATCC G^GATCC
BcgI 737 GCAAAGCGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaHI 2323 GACGTC G[GA]^CG[CT]C
BsiEI 5173 CGGCCG CG[GA][CT]^CG
Bsp1407I 555 TGTACA T^GTACA
BspDI 602 ATCGAT AT^CGAT
BspHI 4913 TCATGA T^CATGA
BsrBI 1178 CCGCTC CCGCTC(-3/-3)
BsrGI 555 TGTACA T^GTACA
ClaI 602 ATCGAT AT^CGAT
DraI 4113 TTTAAA TTT^AAA
DraIII 5502 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1432 GACGGGGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 5173 CGGCCG C^GGCCG
EclXI 5173 CGGCCG C^GGCCG
Eco47III 741 AGCGCT AGC^GCT
Eco52I 5173 CGGCCG C^GGCCG
EcoRV 2845 GATATC GAT^ATC
EcoT22I 83 ATGCAT ATGCA^T
FseI 1900 GGCCGGCC GGCCGG^CC
FspI 1160 TGCGCA TGC^GCA
Hin1I 2323 GACGTC G[GA]^CG[CT]C
KspI 3575 CCGCGG CCGC^GG
MluI 4470 ACGCGT A^CGCGT
NruI 35 TCGCGA TCG^CGA
NsiI 83 ATGCAT ATGCA^T
PaeR7I 3250 CTCGAG C^TCGAG
PshBI 2602 ATTAAT AT^TAAT
Psp1406I 4298 AACGTT AA^CGTT
RcaI 4913 TCATGA T^CATGA
SacII 3575 CCGCGG CCGC^GG
SalI 3118 GTCGAC G^TCGAC
SapI 5470 GAAGAGC GCTCTTC(1/4)
SbfI 4611 CCTGCAGG CCTGCA^GG
ScaI 4244 AGTACT AGT^ACT
SexAI 4624 ACCTGGT A^CC[AT]GGT
SphI 5779 GCATGC GCATG^C
Sse8387I 4611 CCTGCAGG CCTGCA^GG
SspBI 555 TGTACA T^GTACA
SspI 1939 AATATT AAT^ATT
XbaI 511 TCTAGA T^CTAGA
XhoI 3250 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 27 enzymes) #
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Acc65I 173 GGTACC G^GTACC
1040 GGTACC
AhdI 1168 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3975 GACACCCAGTC
Asp700I 39 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3162 GAATAAGTTC
Asp718I 173 GGTACC G^GTACC
1040 GGTACC
AspEI 1168 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3975 GACACCCAGTC
BglII 194 AGATCT A^GATCT
3536 AGATCT
BlpI 4993 GCTCAGC GC^T[ACGT]AGC
5083 GCTAAGC
Bpu1102I 4993 GCTCAGC GC^T[ACGT]AGC
5083 GCTAAGC
BsaWI 610 ACCGGA [AT]^CCGG[AT]
2891 ACCGGA
BssSI 1950 CACGAG CACGAG(-5/-1)
5868 CACGAG
BstAPI 2550 GCAGATTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4959 GCAGACCCTGC
CelII 4993 GCTCAGC GC^T[ACGT]AGC
5083 GCTAAGC
Eam1105I 1168 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3975 GACACCCAGTC
EcoNI 1479 CCTGTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2292 CCTCTGCAAGG
EcoRI 3356 GAATTC G^AATTC
3740 GAATTC
HindIII 267 AAGCTT A^AGCTT
4026 AAGCTT
KpnI 173 GGTACC GGTAC^C
1040 GGTACC
MfeI 2127 CAATTG C^AATTG
2207 CAATTG
MunI 2127 CAATTG C^AATTG
2207 CAATTG
NaeI 1901 GCCGGC GCC^GGC
3208 GCCGGC
NgoMIV 1901 GCCGGC G^CCGGC
3208 GCCGGC
SacI 1953 GAGCTC GAGCT^C
2920 GAGCTC
SmaI 208 CCCGGG CCC^GGG
5984 CCCGGG
StuI 3194 AGGCCT AGG^CCT
3713 AGGCCT
XmaCI 208 CCCGGG C^CCGGG
5984 CCCGGG
XmaI 208 CCCGGG C^CCGGG
5984 CCCGGG
XmnI 39 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3162 GAATAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI ApoI AspHI AspI AvaI
AvaII BalI BanI BanII BbrPI
BbsI BbvCI BbvI BciVI BclI
BcnI BfaI BmyI BpmI BpuAI
BsaAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrDI BsrFI BsrI BssKI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoO109I EcoO65I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflFI PflMI PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII