Restriction map for
pF1KB4032
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# NO CUT ENZYMES ( 80 enzymes) #
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AatII Acc65I AccIII AclI AflII
AgeI Alw44I ApaLI Asp718I AspI
AvaI AviII BciVI BclI BfrI
BsaWI BseAI BsiEI BsiWI BsoBI
BspDI BspEI BsrBI BssSI BstBI
BstEII BstPI ClaI CpoI DrdI
EagI EclXI Eco52I EcoO65I EcoT22I
FbaI FseI FspI HpaI KpnI
KspI MluI MroI MslI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI Psp1406I PvuI
PvuII RsrII SacII SalI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SwaI
Tth111I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AcyI 1869 GGCGCC G[GA]^CG[CT]C
AhdI 781 GACAATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2518 CCTGAGG CC^T[ACGT]AGG
Aor51HI 479 AGCGCT AGC^GCT
ApaI 2659 GGGCCC GGGCC^C
AscI 3315 GGCGCGCC GG^CGCGCC
AseI 1543 ATTAAT AT^TAAT
AsnI 1543 ATTAAT AT^TAAT
Asp700I 2089 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 781 GACAATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 382 CCTAGG C^CTAGG
BalI 1704 TGGCCA TGG^CCA
BamHI 309 GGATCC G^GATCC
BanI 1869 GGCGCC G^G[CT][GA]CC
BbeI 1869 GGCGCC GGCGC^C
BbrPI 613 CACGTG CAC^GTG
BcgI 1670 CGAGAGTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 382 CCTAGG C^CTAGG
BsaBI 1006 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1869 GGCGCC G[GA]^CG[CT]C
BseRI 2608 GAGGAG GAGGAG(10/8)
BsmBI 104 GAGACG CGTCTC(1/5)
Bsp1407I 2841 TGTACA T^GTACA
BspHI 261 TCATGA T^CATGA
BsrDI 635 GCAATG GCAATG(2/0)
BsrFI 947 ACCGGC [GA]^CCGG[CT]
BsrGI 2841 TGTACA T^GTACA
BssHII 3316 GCGCGC G^CGCGC
Bst1107I 1854 GTATAC GTA^TAC
BstXI 1236 CCATCCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1854 GTATAC GTA^TAC
Bsu36I 2518 CCTGAGG CC^T[ACGT]AGG
Cfr10I 947 ACCGGC [GA]^CCGG[CT]
DraI 318 TTTAAA TTT^AAA
DraIII 3138 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 781 GACAATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 479 AGCGCT AGC^GCT
Eco81I 2518 CCTGAGG CC^T[ACGT]AGG
EcoRI 2093 GAATTC G^AATTC
EcoRV 1465 GATATC GAT^ATC
Hin1I 1869 GGCGCC G[GA]^CG[CT]C
HindIII 2269 AAGCTT A^AGCTT
KasI 1869 GGCGCC G^GCGCC
MamI 1006 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 457 CAATTG C^AATTG
MluNI 1704 TGGCCA TGG^CCA
MscI 1704 TGGCCA TGG^CCA
MunI 457 CAATTG C^AATTG
NarI 1869 GGCGCC GG^CGCC
PflMI 1821 CCAGACCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 613 CACGTG CAC^GTG
PmlI 613 CACGTG CAC^GTG
PshBI 1543 ATTAAT AT^TAAT
RcaI 261 TCATGA T^CATGA
SacI 697 GAGCTC GAGCT^C
SapI 2902 GCTCTTC GCTCTTC(1/4)
SfoI 1869 GGCGCC GGC^GCC
SphI 2681 GCATGC GCATG^C
SspBI 2841 TGTACA T^GTACA
SspI 2260 AATATT AAT^ATT
Van91I 1821 CCAGACCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 2089 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AflIII 2394 ACATGT A^C[GA][CT]GT
2586 ACATGT
BbsI 2668 GAAGAC GAAGAC(2/6)
3286 GAAGAC
BbvCI 939 GCTGAGG CCTCAGC(-5/-2)
1356 GCTGAGG
BpuAI 2668 GAAGAC GAAGAC(2/6)
3286 GAAGAC
BsaAI 613 CACGTG [CT]AC^GT[GA]
3347 CACGTA
BsgI 1897 GTGCAG GTGCAG(16/14)
2921 GTGCAG
BspLU11I 2394 ACATGT A^CATGT
2586 ACATGT
BspMI 1749 ACCTGC ACCTGC(4/8)
2706 GCAGGT
BstAPI 453 GCAACAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1890 GCAGGCTGTGC
EarI 2903 CTCTTC CTCTTC(1/4)
624 GAAGAG
EcoNI 813 CCTCTATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2494 CCTTCTCCAGG
FauI 2688 CCCGC CCCGC(4/6)
1473 GCGGG
HaeII 479 AGCGCT [GA]GCGC^[CT]
1869 GGCGCC
HgaI 1030 GCGTC GACGC(5/10)
1380 GCGTC
HincII 1617 GTCAAC GT[CT]^[GA]AC
1908 GTTGAC
HindII 1617 GTCAAC GT[CT]^[GA]AC
1908 GTTGAC
Ksp632I 2903 CTCTTC CTCTTC(1/4)
624 GAAGAG
MspA1I 1354 CCGCTG C[AC]G^C[GT]G
3065 CCGCTG
NcoI 1634 CCATGG C^CATGG
2300 CCATGG
PpuMI 1059 AGGTCCC [GA]G^G[AT]CC[CT]
2244 GGGTCCT
SmlI 751 CTCAAG C^T[CT][GA]AG
1098 CTCAAG
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# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaII BanII BbvI BcnI BfaI
BglI BglII BlpI BmyI BpmI
Bpu1102I BsaI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII