Restriction map for
pF1KB5414
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII AccI AccII AclI AcyI
AflIII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I AspEI AspHI
AspI AviII AvrII BalI BamHI
BanII BbeI BbrPI BbvCI BcgI
BciVI BclI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsgI
BsiEI BsiHKAI BsiWI BsiYI BslI
BsmFI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KspI
MamI MfeI MluI MluNI MscI
MslI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tsp45I Tth111I Van91I XbaI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 589 GGTACC G^GTACC
AccIII 292 TCCGGA T^CCGGA
AciI 214 GCGG CCGC(-3/-1)
AflII 994 CTTAAG C^TTAAG
Asp718I 589 GGTACC G^GTACC
AvaI 890 CTCGAG C^[CT]CG[GA]G
BbsI 261 GTCTTC GAAGAC(2/6)
BcnI 649 CCGGG CC^[GC]GG
BfrI 994 CTTAAG C^TTAAG
BglI 174 GCCCAATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 695 AGATCT A^GATCT
BmyI 703 GTGCCC G[AGT]GC[ACT]^C
BpuAI 261 GTCTTC GAAGAC(2/6)
BsaWI 292 TCCGGA [AT]^CCGG[AT]
BseAI 292 TCCGGA T^CCGGA
BsmBI 740 GAGACG CGTCTC(1/5)
BsoBI 890 CTCGAG C^[CT]CG[GA]G
Bsp1286I 703 GTGCCC G[AGT]GC[ACT]^C
BspEI 292 TCCGGA T^CCGGA
BspMI 486 ACCTGC ACCTGC(4/8)
BstAPI 127 GCAGCTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 806 CATCC GGATG(2/0)
BstXI 27 CCAGCAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 695 AGATCT [GA]^GATC[CT]
DpnII 696 GATC ^GATC
FokI 806 CATCC GGATG(9/13)
HphI 494 TCACC GGTGA(8/7)
KpnI 589 GGTACC GGTAC^C
MaeII 743 ACGT A^CGT
MboI 696 GATC ^GATC
MflI 695 AGATCT [GA]^GATC[CT]
MroI 292 TCCGGA T^CCGGA
NciI 649 CCGGG CC^[GC]GG
NdeII 696 GATC ^GATC
PaeR7I 890 CTCGAG C^TCGAG
PpuMI 998 AGGACCT [GA]G^G[AT]CC[CT]
Sau3AI 696 GATC ^GATC
StuI 391 AGGCCT AGG^CCT
TaqI 891 TCGA T^CGA
TthHB8I 891 TCGA T^CGA
XcmI 525 CCAAACTAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 890 CTCGAG C^TCGAG
XhoII 695 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcsI 377 AAATTC [GA]^AATT[CT]
407 AAATTT
ApoI 377 AAATTC [GA]^AATT[CT]
407 AAATTT
AvaII 861 GGTCC G^G[AT]CC
999 GGACC
BanI 507 GGTGCC G^G[CT][GA]CC
589 GGTACC
DdeI 644 CTCAG C^T[ACGT]AG
699 CTTAG
DraII 605 AGGGCCT [GA]G^G[ACGT]CC[CT]
998 AGGACCT
DsaI 5 CCATGG C^C[GA][CT]GG
198 CCATGG
EarI 457 GAAGAG CTCTTC(1/4)
469 GAAGAG
EcoO109I 605 AGGGCCT [GA]G^G[ACGT]CC[CT]
998 AGGACCT
HapII 293 CCGG C^CGG
649 CCGG
HpaII 293 CCGG C^CGG
649 CCGG
Ksp632I 457 GAAGAG CTCTTC(1/4)
469 GAAGAG
MspI 293 CCGG C^CGG
649 CCGG
NcoI 5 CCATGG C^CATGG
198 CCATGG
PleI 203 GAGTC GAGTC(4/5)
337 GAGTC
SfcI 399 CTACAG C^T[GA][CT]AG
664 CTACAG
SmlI 890 CTCGAG C^T[CT][GA]AG
994 CTTAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AfaI AluI AlwNI BbvI BfaI
BpmI BsaJI BseRI BsmAI BssKI
BstNI Cac8I Cfr13I EcoRII EcoT14I
Fnu4HI HaeIII HinfI ItaI MaeI
MaeIII MboII MnlI MseI MspA1I
MvaI MwoI NlaIII NlaIV PvuII
RsaI Sau96I ScrFI SfaNI StyI
TfiI Tru9I TseI Tsp509I TspRI