Restriction map for
pF1KB5549
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# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AspEI
AviII AvrII BbrPI BcgI BfrI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsiWI Bsp1407I BspLU11I BsrDI BsrGI
BstAPI BstBI BstEII BstPI CelII
CpoI Eam1105I Eco47III EcoO65I FspI
MamI MluI NdeI NruI NspV
PflMI PinAI PmaCI PmeI PmlI
Psp1406I PstI PvuI RsrII SalI
SapI SbfI ScaI SfuI SnaBI
SpeI SphI Sse8387I SspBI StuI
Van91I XcmI
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# ONE CUT ENZYMES ( 63 enzymes) #
###################################
Acc65I 3794 GGTACC G^GTACC
AccIII 3525 TCCGGA T^CCGGA
AflIII 524 ACGTGT A^C[GA][CT]GT
AocI 322 CCTGAGG CC^T[ACGT]AGG
ApaI 352 GGGCCC GGGCC^C
Asp718I 3794 GGTACC G^GTACC
AspI 426 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 3516 TGGCCA TGG^CCA
BamHI 731 GGATCC G^GATCC
BbeI 139 GGCGCC GGCGC^C
BbsI 937 GAAGAC GAAGAC(2/6)
BbvCI 201 GCTGAGG CCTCAGC(-5/-2)
BclI 1118 TGATCA T^GATCA
BglII 3604 AGATCT A^GATCT
BpuAI 937 GAAGAC GAAGAC(2/6)
BseAI 3525 TCCGGA T^CCGGA
BsgI 3569 CTGCAC GTGCAG(16/14)
BsmBI 2497 CGTCTC CGTCTC(1/5)
BspDI 2792 ATCGAT AT^CGAT
BspEI 3525 TCCGGA T^CCGGA
BssHII 714 GCGCGC G^CGCGC
BssSI 3173 CTCGTG CACGAG(-5/-1)
Bst1107I 3538 GTATAC GTA^TAC
BstZ17I 3538 GTATAC GTA^TAC
Bsu36I 322 CCTGAGG CC^T[ACGT]AGG
ClaI 2792 ATCGAT AT^CGAT
DraI 862 TTTAAA TTT^AAA
DraIII 2461 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1762 GACCCTGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 322 CCTGAGG CC^T[ACGT]AGG
EcoNI 693 CCTGGACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1796 GAATTC G^AATTC
EcoRV 1626 GATATC GAT^ATC
FbaI 1118 TGATCA T^GATCA
HpaI 2029 GTTAAC GTT^AAC
KasI 139 GGCGCC G^GCGCC
KpnI 3794 GGTACC GGTAC^C
MaeII 524 ACGT A^CGT
MfeI 3067 CAATTG C^AATTG
MluNI 3516 TGGCCA TGG^CCA
MroI 3525 TCCGGA T^CCGGA
MscI 3516 TGGCCA TGG^CCA
MunI 3067 CAATTG C^AATTG
NarI 139 GGCGCC GG^CGCC
NcoI 2979 CCATGG C^CATGG
NspI 410 ACATGC [GA]CATG^[CT]
PacI 2713 TTAATTAA TTAAT^TAA
PaeR7I 2537 CTCGAG C^TCGAG
PflFI 426 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 488 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 3056 CAGCTG CAG^CTG
SexAI 1653 ACCAGGT A^CC[AT]GGT
SfcI 834 CTATAG C^T[GA][CT]AG
SfiI 223 GGCCTCCCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 139 GGCGCC GGC^GCC
SgrAI 675 CACCGGCG C[GA]^CCGG[CT]G
SmaI 86 CCCGGG CCC^GGG
SwaI 861 ATTTAAAT ATTT^AAAT
Tth111I 426 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2400 TCTAGA T^CTAGA
XhoI 2537 CTCGAG C^TCGAG
XmaCI 86 CCCGGG C^CCGGG
XmaI 86 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AseI 1219 ATTAAT AT^TAAT
2455 ATTAAT
AsnI 1219 ATTAAT AT^TAAT
2455 ATTAAT
Asp700I 1132 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2179 GAATATTTTC
BpmI 809 CTCCAG CTGGAG(16/14)
2483 CTGGAG
BsaI 424 GAGACC GGTCTC(1/5)
3223 GAGACC
BsaWI 3525 TCCGGA [AT]^CCGG[AT]
3872 ACCGGA
BsmI 1194 GAATGC GAATGC(1/-1)
2234 GAATGC
BspHI 3828 TCATGA T^CATGA
4020 TCATGA
DraII 336 GGGGCCT [GA]G^G[ACGT]CC[CT]
488 GGGACCC
EcoO109I 336 GGGGCCT [GA]G^G[ACGT]CC[CT]
488 GGGACCC
EcoT22I 412 ATGCAT ATGCA^T
1687 ATGCAT
FseI 271 GGCCGGCC GGCCGG^CC
382 GGCCGGCC
HindIII 654 AAGCTT A^AGCTT
878 AAGCTT
KspI 218 CCGCGG CCGC^GG
483 CCGCGG
NheI 1429 GCTAGC G^CTAGC
2659 GCTAGC
NsiI 412 ATGCAT ATGCA^T
1687 ATGCAT
PshAI 625 GACAATAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3262 GACTTTAGTC
PshBI 1219 ATTAAT AT^TAAT
2455 ATTAAT
RcaI 3828 TCATGA T^CATGA
4020 TCATGA
SacI 78 GAGCTC GAGCT^C
3128 GAGCTC
SacII 218 CCGCGG CCGC^GG
483 CCGCGG
XmnI 1132 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2179 GAATATTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApoI
AspHI AvaI AvaII BanI BanII
BbvI BciVI BcnI BfaI BglI
BmyI BsaHI BsaJI BseRI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DsaI EaeI
EagI EarI EclXI Eco52I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NotI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII