Restriction map for
pF1KB5593
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# NO CUT ENZYMES ( 80 enzymes) #
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AccIII AclI AgeI AhdI Alw44I
Aor51HI ApaLI AscI AspEI AviII
AvrII BbeI BbrPI BcgI BcnI
BlnI BsaAI BsaBI BsaWI BseAI
BsiEI BsiWI BspDI BspEI BsrBI
BsrFI BssHII BstBI Cfr10I ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoRV FseI FspI
HapII HindIII HpaII KasI KspI
MamI MluI MroI MspI NaeI
NarI NciI NdeI NgoMIV NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacII SalI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
Sse8387I SwaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 525 GACGTC GACGT^C
Acc65I 2313 GGTACC G^GTACC
AccII 19 CGCG CG^CG
AcyI 525 GACGTC G[GA]^CG[CT]C
AflII 1419 CTTAAG C^TTAAG
AflIII 979 ACATGT A^C[GA][CT]GT
ApaI 5308 GGGCCC GGGCC^C
Asp718I 2313 GGTACC G^GTACC
AspI 5157 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 5501 CCCGAG C^[CT]CG[GA]G
BalI 1295 TGGCCA TGG^CCA
BamHI 3939 GGATCC G^GATCC
BbvCI 1830 GCTGAGG CCTCAGC(-5/-2)
BclI 89 TGATCA T^GATCA
BfrI 1419 CTTAAG C^TTAAG
BglI 1974 GCCAGTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 525 GACGTC G[GA]^CG[CT]C
BsaI 1518 GAGACC GGTCTC(1/5)
BsoBI 5501 CCCGAG C^[CT]CG[GA]G
BspLU11I 979 ACATGT A^CATGT
BsrDI 4879 GCAATG GCAATG(2/0)
Bst1107I 2936 GTATAC GTA^TAC
BstUI 19 CGCG CG^CG
BstZ17I 2936 GTATAC GTA^TAC
DraI 3106 TTTAAA TTT^AAA
DraIII 108 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 4952 CCATGG C^C[GA][CT]GG
EaeI 1295 TGGCCA [CT]^GGCC[GA]
EcoRI 907 GAATTC G^AATTC
EcoT22I 3422 ATGCAT ATGCA^T
FauI 5122 GCGGG CCCGC(4/6)
FbaI 89 TGATCA T^GATCA
HaeII 2594 AGCGCC [GA]GCGC^[CT]
Hin1I 525 GACGTC G[GA]^CG[CT]C
KpnI 2313 GGTACC GGTAC^C
MluNI 1295 TGGCCA TGG^CCA
MscI 1295 TGGCCA TGG^CCA
MvnI 19 CGCG CG^CG
NcoI 4952 CCATGG C^CATGG
NheI 3877 GCTAGC G^CTAGC
NsiI 3422 ATGCAT ATGCA^T
PflFI 5157 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 525 GACGTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 4579 GAGCTC GAGCT^C
ScaI 3148 AGTACT AGT^ACT
SphI 140 GCATGC GCATG^C
Tth111I 5157 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4671 TCTAGA T^CTAGA
XcmI 5327 CCACAGATCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 30 enzymes) #
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AocI 1540 CCTGAGG CC^T[ACGT]AGG
3271 CCTGAGG
AspHI 3069 GAGCAC G[AT]GC[AT]^C
4579 GAGCTC
BanI 2313 GGTACC G^G[CT][GA]CC
5487 GGCACC
BlpI 935 GCTTAGC GC^T[ACGT]AGC
3555 GCTTAGC
Bpu1102I 935 GCTTAGC GC^T[ACGT]AGC
3555 GCTTAGC
BsiHKAI 3069 GAGCAC G[AT]GC[AT]^C
4579 GAGCTC
BsmBI 4131 CGTCTC CGTCTC(1/5)
688 GAGACG
Bsp1407I 2757 TGTACA T^GTACA
4994 TGTACA
BspHI 1308 TCATGA T^CATGA
1656 TCATGA
BspMI 2019 ACCTGC ACCTGC(4/8)
4322 ACCTGC
BsrGI 2757 TGTACA T^GTACA
4994 TGTACA
BssSI 4763 CTCGTG CACGAG(-5/-1)
4990 CTCGTG
Bsu36I 1540 CCTGAGG CC^T[ACGT]AGG
3271 CCTGAGG
CelII 935 GCTTAGC GC^T[ACGT]AGC
3555 GCTTAGC
CfoI 1864 GCGC GCG^C
2595 GCGC
Eco81I 1540 CCTGAGG CC^T[ACGT]AGG
3271 CCTGAGG
EcoNI 1522 CCTACATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3852 CCTTAGTGAGG
HhaI 1864 GCGC GCG^C
2595 GCGC
HinP1I 1864 GCGC G^CGC
2595 GCGC
HpaI 2103 GTTAAC GTT^AAC
2509 GTTAAC
MaeII 526 ACGT A^CGT
1241 ACGT
PflMI 375 CCAAAGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5541 CCAGGAGCTGG
PpuMI 2668 GGGTCCT [GA]G^G[AT]CC[CT]
4319 AGGACCT
RcaI 1308 TCATGA T^CATGA
1656 TCATGA
SpeI 1872 ACTAGT A^CTAGT
3487 ACTAGT
SspBI 2757 TGTACA T^GTACA
4994 TGTACA
StuI 1530 AGGCCT AGG^CCT
4410 AGGCCT
TaqI 1721 TCGA T^CGA
1787 TCGA
TthHB8I 1721 TCGA T^CGA
1787 TCGA
Van91I 375 CCAAAGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5541 CCAGGAGCTGG
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# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AseI AsnI
Asp700I AvaII BanII BbsI BbvI
BciVI BfaI BglII BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BsrI BssKI BstAPI BstEII BstF5I
BstNI BstPI BstXI BstYI Cac8I
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoO65I EcoRII EcoT14I Fnu4HI
FokI HaeIII HgaI HincII HindII
HinfI HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MfeI MflI
MnlI MseI MslI MspA1I MunI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PshBI PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SmlI SspI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII XmnI