Restriction map for
pF1KB7007
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# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AocI AscI AseI AsnI AvrII
BcgI BciVI BclI BfrI BlnI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BsrBI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I ClaI DraIII EagI
EclXI Eco52I Eco81I EcoO65I EcoRV
FbaI FseI KspI MroI NheI
NotI NruI NspV PacI PinAI
PmeI PshBI Psp1406I PvuI RcaI
SacII SapI SexAI SfuI SgrAI
SnaBI SpeI SspBI SwaI XbaI
XcmI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 950 GGTACC G^GTACC
AccII 3197 CGCG CG^CG
Alw44I 305 GTGCAC G^TGCAC
Aor51HI 2622 AGCGCT AGC^GCT
ApaLI 305 GTGCAC G^TGCAC
Asp718I 950 GGTACC G^GTACC
AviII 974 TGCGCA TGC^GCA
BamHI 1332 GGATCC G^GATCC
BbeI 715 GGCGCC GGCGC^C
BbrPI 1266 CACGTG CAC^GTG
BglII 3104 AGATCT A^GATCT
BlpI 1188 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1188 GCTGAGC GC^T[ACGT]AGC
BsaAI 1266 CACGTG [CT]AC^GT[GA]
BsaBI 1519 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2514 GAGACC GGTCTC(1/5)
BsaWI 2295 ACCGGA [AT]^CCGG[AT]
BsmI 2361 GAATGC GAATGC(1/-1)
BsrFI 277 GCCGGC [GA]^CCGG[CT]
BstUI 3197 CGCG CG^CG
BstXI 2600 CCACGGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1188 GCTGAGC GC^T[ACGT]AGC
Cfr10I 277 GCCGGC [GA]^CCGG[CT]
CpoI 380 CGGACCG CG^G[AT]CCG
DraI 1087 TTTAAA TTT^AAA
Eco47III 2622 AGCGCT AGC^GCT
EcoRI 2983 GAATTC G^AATTC
EcoT22I 2111 ATGCAT ATGCA^T
FspI 974 TGCGCA TGC^GCA
KasI 715 GGCGCC G^GCGCC
KpnI 950 GGTACC GGTAC^C
MamI 1519 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4094 CAATTG C^AATTG
MluI 3196 ACGCGT A^CGCGT
MunI 4094 CAATTG C^AATTG
MvnI 3197 CGCG CG^CG
NaeI 277 GCCGGC GCC^GGC
NarI 715 GGCGCC GG^CGCC
NdeI 3565 CATATG CA^TATG
NgoMIV 277 GCCGGC G^CCGGC
NsiI 2111 ATGCAT ATGCA^T
PaeR7I 581 CTCGAG C^TCGAG
PmaCI 1266 CACGTG CAC^GTG
PmlI 1266 CACGTG CAC^GTG
PpuMI 542 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 1243 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 380 CGGACCG CG^G[AT]CCG
SacI 517 GAGCTC GAGCT^C
SbfI 660 CCTGCAGG CCTGCA^GG
ScaI 2960 AGTACT AGT^ACT
SfiI 658 GGCCTGCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 715 GGCGCC GGC^GCC
Sse8387I 660 CCTGCAGG CCTGCA^GG
StuI 344 AGGCCT AGG^CCT
XhoI 581 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 27 enzymes) #
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AcyI 715 GGCGCC G[GA]^CG[CT]C
1018 GACGCC
AhdI 3862 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3949 GACGAAAAGTC
AspEI 3862 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3949 GACGAAAAGTC
BbsI 1072 GAAGAC GAAGAC(2/6)
41 GTCTTC
BglI 659 GCCTGCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2597 GCCCCACGGGC
BpuAI 1072 GAAGAC GAAGAC(2/6)
41 GTCTTC
BsaHI 715 GGCGCC G[GA]^CG[CT]C
1018 GACGCC
BspLU11I 1172 ACATGT A^CATGT
1971 ACATGT
BsrDI 1876 GCAATG GCAATG(2/0)
2321 GCAATG
BssSI 336 CTCGTG CACGAG(-5/-1)
520 CTCGTG
BstAPI 2683 GCAAATCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2957 GCAAGTACTGC
BstYI 1332 GGATCC [GA]^GATC[CT]
3104 AGATCT
DrdI 1018 GACGCCTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1282 GACCCCCCCGTC
Eam1105I 3862 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3949 GACGAAAAGTC
Hin1I 715 GGCGCC G[GA]^CG[CT]C
1018 GACGCC
HindIII 1853 AAGCTT A^AGCTT
3049 AAGCTT
HpaI 1065 GTTAAC GTT^AAC
1116 GTTAAC
MflI 1332 GGATCC [GA]^GATC[CT]
3104 AGATCT
NcoI 119 CCATGG C^CATGG
1965 CCATGG
PflMI 483 CCAGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
561 CCAGCGTCTGG
SmaI 3272 CCCGGG CCC^GGG
3827 CCCGGG
SphI 331 GCATGC GCATG^C
1193 GCATGC
SspI 2265 AATATT AAT^ATT
4169 AATATT
Van91I 483 CCAGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
561 CCAGCGTCTGG
XhoII 1332 GGATCC [GA]^GATC[CT]
3104 AGATCT
XmaCI 3272 CCCGGG C^CCGGG
3827 CCCGGG
XmaI 3272 CCCGGG C^CCGGG
3827 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApaI ApoI
Asp700I AspHI AspI AvaI AvaII
BalI BanI BanII BbvCI BbvI
BcnI BfaI BmyI BpmI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflFI PleI PstI
PvuII RsaI SalI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I XmnI