Restriction map for
pF1KB8002
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# NO CUT ENZYMES ( 178 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AvaII
AviII AvrII BalI BamHI BanII
BbeI BbrPI BbvCI BcgI BciVI
BcnI BglI BglII BlnI BlpI
BmyI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaJI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstF5I BstUI BstXI BstZ17I
Bsu36I Cac8I CelII CfoI Cfr10I
ClaI CpoI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoRV EcoT14I EcoT22I FauI FokI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII KasI KpnI
Ksp632I KspI MaeII MamI MfeI
MluI MluNI MroI MscI MspI
MunI MvnI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I SspBI
SspI StyI SwaI Tth111I Van91I
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AflII 862 CTTAAG C^TTAAG
AlwNI 974 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 460 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 643 GGCACC G^G[CT][GA]CC
BclI 1122 TGATCA T^GATCA
BfrI 862 CTTAAG C^TTAAG
BsaI 717 GGTCTC GGTCTC(1/5)
BseRI 922 GAGGAG GAGGAG(10/8)
BsrDI 199 GCAATG GCAATG(2/0)
BssKI 153 CCTGG ^CC[ACGT]GG
BstEII 406 GGTTACC G^GT[ACGT]ACC
BstNI 153 CCTGG CC^[AT]GG
BstPI 406 GGTTACC G^GT[ACGT]ACC
BstYI 363 AGATCC [GA]^GATC[CT]
Cfr13I 1056 GGCCC G^G[ACGT]CC
DraI 1009 TTTAAA TTT^AAA
EcoNI 153 CCTGGATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 406 GGTTACC G^GT[ACGT]ACC
EcoRI 825 GAATTC G^AATTC
EcoRII 153 CCTGG ^CC[AT]GG
FbaI 1122 TGATCA T^GATCA
HphI 1117 GGTGA GGTGA(8/7)
MflI 363 AGATCC [GA]^GATC[CT]
MspA1I 209 CAGCTG C[AC]G^C[GT]G
MvaI 153 CCTGG CC^[AT]GG
NdeI 878 CATATG CA^TATG
PvuII 209 CAGCTG CAG^CTG
Sau96I 1056 GGCCC G^G[ACGT]CC
ScrFI 153 CCTGG CC^[ACGT]GG
SfcI 722 CTACAG C^T[GA][CT]AG
SmlI 862 CTTAAG C^T[CT][GA]AG
SpeI 601 ACTAGT A^CTAGT
StuI 70 AGGCCT AGG^CCT
XbaI 1096 TCTAGA T^CTAGA
XcmI 877 CCATATGGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 363 AGATCC [GA]^GATC[CT]
XmnI 460 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AciI 4 GCGG CCGC(-3/-1)
989 GCGG
AluI 210 AGCT AG^CT
978 AGCT
AlwI 364 GATCC GGATC(4/5)
128 GGATC
BbsI 495 GAAGAC GAAGAC(2/6)
1063 GTCTTC
BpuAI 495 GAAGAC GAAGAC(2/6)
1063 GTCTTC
BsiYI 153 CCTGGATAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
367 CCTTCAAAGGG
BslI 153 CCTGGATAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
367 CCTTCAAAGGG
BspHI 87 TCATGA T^CATGA
918 TCATGA
HaeIII 71 GGCC GG^CC
1056 GGCC
MslI 440 CATCTCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
598 CACACTAGTG
MwoI 199 GCAATGGAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
214 GCCCTGAAAGC
NlaIII 88 CATG CATG^
919 CATG
PleI 256 GAGTC GAGTC(4/5)
913 GAGTC
RcaI 87 TCATGA T^CATGA
918 TCATGA
SfaNI 181 GCATC GCATC(5/9)
439 GCATC
TaqI 313 TCGA T^CGA
853 TCGA
TthHB8I 313 TCGA T^CGA
853 TCGA
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# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AcsI ApoI BbvI BfaI BsmAI
BsrI DdeI DpnII Fnu4HI HinfI
ItaI MaeI MaeIII MboI MboII
MnlI MseI NdeII NlaIV Sau3AI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI