Restriction map for
pF1KB8003
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# NO CUT ENZYMES ( 171 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
Alw44I AlwI AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspHI AspI AvaI AviII
AvrII BalI BamHI BbeI BbrPI
BbvCI BcgI BciVI BfaI BfrI
BglI BglII BlnI BsaAI BsaBI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EaeI EagI EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HaeII HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MaeII MamI MfeI MflI
MluI MluNI MroI MscI MseI
MslI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tru9I Tsp509I Tth111I TthHB8I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AcyI 406 GACGCC G[GA]^CG[CT]C
AfaI 66 GTAC GT^AC
AhdI 304 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 330 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 442 GGGCCC GGGCC^C
AspEI 304 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 367 GGTGCC G^G[CT][GA]CC
BanII 442 GGGCCC G[GA]GC[CT]^C
BbsI 403 GAAGAC GAAGAC(2/6)
BclI 263 TGATCA T^GATCA
BlpI 341 GCTCAGC GC^T[ACGT]AGC
BmyI 442 GGGCCC G[AGT]GC[ACT]^C
BpmI 256 CTGGAG CTGGAG(16/14)
Bpu1102I 341 GCTCAGC GC^T[ACGT]AGC
BpuAI 403 GAAGAC GAAGAC(2/6)
BsaHI 406 GACGCC G[GA]^CG[CT]C
BsaI 359 GAGACC GGTCTC(1/5)
Bsp1286I 442 GGGCCC G[AGT]GC[ACT]^C
BspMI 176 ACCTGC ACCTGC(4/8)
CelII 341 GCTCAGC GC^T[ACGT]AGC
DraII 251 AGGCCCT [GA]G^G[ACGT]CC[CT]
Eam1105I 304 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 251 AGGCCCT [GA]G^G[ACGT]CC[CT]
FauI 440 GCGGG CCCGC(4/6)
FbaI 263 TGATCA T^GATCA
HgaI 406 GACGC GACGC(5/10)
Hin1I 406 GACGCC G[GA]^CG[CT]C
MaeIII 198 GTCAC ^GT[ACGT]AC
NlaIII 42 CATG CATG^
PflMI 92 CCAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 310 GAGTC GAGTC(4/5)
PstI 336 CTGCAG CTGCA^G
RsaI 66 GTAC GT^AC
SfcI 336 CTGCAG C^T[GA][CT]AG
TfiI 457 GATTC G^A[AT]TC
Tsp45I 198 GTCAC ^GT[GC]AC
Van91I 92 CCAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 353 CCAGGGGAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
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AvaII 162 GGTCC G^G[AT]CC
239 GGACC
BcnI 25 CCCGG CC^[GC]GG
236 CCCGG
BsmAI 359 GAGAC GTCTC(1/5)
312 GTCTC
BsrBI 103 GAGCGG CCGCTC(-3/-3)
438 GAGCGG
BsrI 96 CCAGT ACTGG(1/-1)
195 CCAGT
CfoI 39 GCGC GCG^C
418 GCGC
EcoT14I 242 CCAAGG C^C[AT][AT]GG
347 CCAAGG
HhaI 39 GCGC GCG^C
418 GCGC
HinP1I 39 GCGC G^CGC
418 GCGC
HinfI 310 GAGTC G^A[ACGT]TC
457 GATTC
HphI 183 GGTGA GGTGA(8/7)
233 TCACC
MspA1I 111 CAGCGG C[AC]G^C[GT]G
165 CCGCTG
NciI 25 CCCGG CC^[GC]GG
236 CCCGG
SfaNI 387 GATGC GCATC(5/9)
464 GCATC
StyI 242 CCAAGG C^C[AT][AT]GG
347 CCAAGG
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# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AluI BbvI BsaJI BseRI
BsiYI BslI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HapII HpaII
ItaI MboI MboII MnlI MspI
MvaI MwoI NdeII NlaIV Sau3AI
Sau96I ScrFI TseI TspRI