Restriction map for
pF1KB8004
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# NO CUT ENZYMES ( 98 enzymes) #
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AatII AccIII AclI AcsI AflII
AflIII AgeI AhdI Alw44I ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AspI AviII AvrII BbrPI
BbvCI BclI BfrI BlnI BlpI
Bpu1102I BsaAI BseAI BsiWI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I CelII ClaI
CpoI DrdI Eam1105I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HpaI MfeI MluI
MroI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SalI SexAI SfiI
SfuI SgrAI SnaBI SpeI SspBI
SspI StuI SwaI Tsp509I Tth111I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AocI 1183 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1782 AGCGCT AGC^GCT
ApaI 1612 GGGCCC GGGCC^C
BalI 2 TGGCCA TGG^CCA
BamHI 1121 GGATCC G^GATCC
BbeI 1959 GGCGCC GGCGC^C
BciVI 437 GTATCC GTATCC(6/5)
BglII 1088 AGATCT A^GATCT
BsaBI 387 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1692 GGTCTC GGTCTC(1/5)
BsgI 1235 CTGCAC GTGCAG(16/14)
BsiEI 1049 CGGCCG CG[GA][CT]^CG
BsmBI 1044 CGTCTC CGTCTC(1/5)
BsmI 267 GAATGC GAATGC(1/-1)
BspHI 800 TCATGA T^CATGA
BsrBI 612 CCGCTC CCGCTC(-3/-3)
BsrDI 365 GCAATG GCAATG(2/0)
BsrFI 2000 ACCGGC [GA]^CCGG[CT]
BstXI 2019 CCACAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1183 CCTGAGG CC^T[ACGT]AGG
Cfr10I 2000 ACCGGC [GA]^CCGG[CT]
DraI 865 TTTAAA TTT^AAA
EagI 1049 CGGCCG C^GGCCG
EclXI 1049 CGGCCG C^GGCCG
Eco47III 1782 AGCGCT AGC^GCT
Eco52I 1049 CGGCCG C^GGCCG
Eco81I 1183 CCTGAGG CC^T[ACGT]AGG
KasI 1959 GGCGCC G^GCGCC
KspI 1608 CCGCGG CCGC^GG
MamI 387 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MseI 866 TTAA T^TAA
NarI 1959 GGCGCC GG^CGCC
NcoI 1688 CCATGG C^CATGG
PflMI 1633 CCACTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 1359 CAGCTG CAG^CTG
RcaI 800 TCATGA T^CATGA
SacII 1608 CCGCGG CCGC^GG
SbfI 1464 CCTGCAGG CCTGCA^GG
ScaI 1565 AGTACT AGT^ACT
SfoI 1959 GGCGCC GGC^GCC
SphI 135 GCATGC GCATG^C
Sse8387I 1464 CCTGCAGG CCTGCA^GG
Tru9I 866 TTAA T^TAA
Van91I 1633 CCACTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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Acc65I 61 GGTACC G^GTACC
1421 GGTACC
AccI 520 GTCTAC GT^[AC][GT]AC
727 GTAGAC
AccII 1609 CGCG CG^CG
2095 CGCG
Asp718I 61 GGTACC G^GTACC
1421 GGTACC
BbsI 949 GAAGAC GAAGAC(2/6)
1409 GTCTTC
BcgI 272 CGACCCCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
639 GCAGCACTATCG
BglI 1951 GCCAAGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1968 GCCTGAACGGC
BpuAI 949 GAAGAC GAAGAC(2/6)
1409 GTCTTC
BsaWI 1289 TCCGGT [AT]^CCGG[AT]
1725 ACCGGA
BstUI 1609 CGCG CG^CG
2095 CGCG
DraIII 238 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
1634 CACTGAGTG
EaeI 2 TGGCCA [CT]^GGCC[GA]
1049 CGGCCG
EarI 1924 CTCTTC CTCTTC(1/4)
349 GAAGAG
HindIII 294 AAGCTT A^AGCTT
631 AAGCTT
KpnI 61 GGTACC GGTAC^C
1421 GGTACC
Ksp632I 1924 CTCTTC CTCTTC(1/4)
349 GAAGAG
MslI 1788 CACCCGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1914 CACGGCTGTG
MvnI 1609 CGCG CG^CG
2095 CGCG
NspI 135 GCATGC [GA]CATG^[CT]
1485 GCATGT
SacI 1675 GAGCTC GAGCT^C
1719 GAGCTC
SapI 349 GAAGAGC GCTCTTC(1/4)
1923 GCTCTTC
SmaI 26 CCCGGG CCC^GGG
1397 CCCGGG
TaqI 896 TCGA T^CGA
1526 TCGA
TthHB8I 896 TCGA T^CGA
1526 TCGA
XmaCI 26 CCCGGG C^CCGGG
1397 CCCGGG
XmaI 26 CCCGGG C^CCGGG
1397 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BanI
BanII BbvI BcnI BfaI BmyI
BpmI BsaHI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MnlI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TfiI TseI Tsp45I
TspRI XcmI XhoII