Restriction map for
pF1KSDA0007
##################################
# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII AccIII AclI AflII AflIII
Alw44I AocI Aor51HI ApaLI AscI
Asp700I AspHI AspI AvaI AviII
AvrII BalI BamHI BbrPI BbvCI
BcgI BciVI BclI BcnI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BseAI BsiEI BsiHKAI BsiWI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraIII DsaI EagI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV FbaI
FseI FspI HgaI KspI MamI
MfeI MluI MluNI MroI MscI
MunI NaeI NciI NcoI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AccI 598 GTCTAC GT^[AC][GT]AC
AcsI 1191 GAATTC [GA]^AATT[CT]
AcyI 201 GGCGCC G[GA]^CG[CT]C
AgeI 728 ACCGGT A^CCGGT
AhdI 1027 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 2005 GATCC GGATC(4/5)
ApaI 1142 GGGCCC GGGCC^C
ApoI 1191 GAATTC [GA]^AATT[CT]
AseI 357 ATTAAT AT^TAAT
AsnI 357 ATTAAT AT^TAAT
AspEI 1027 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanII 1142 GGGCCC G[GA]GC[CT]^C
BbeI 201 GGCGCC GGCGC^C
BglI 204 GCCAGCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 1142 GGGCCC G[AGT]GC[ACT]^C
BsaAI 154 TACGTG [CT]AC^GT[GA]
BsaHI 201 GGCGCC G[GA]^CG[CT]C
BsaI 586 GAGACC GGTCTC(1/5)
BsaWI 728 ACCGGT [AT]^CCGG[AT]
BsmBI 124 GAGACG CGTCTC(1/5)
Bsp1286I 1142 GGGCCC G[AGT]GC[ACT]^C
BsrBI 1250 CCGCTC CCGCTC(-3/-3)
BsrDI 1330 GCAATG GCAATG(2/0)
BstYI 2004 AGATCC [GA]^GATC[CT]
DraI 1089 TTTAAA TTT^AAA
DrdI 376 GACAACAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 128 CGGCCA [CT]^GGCC[GA]
Eam1105I 1027 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 964 CCTGGGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1191 GAATTC G^AATTC
EcoT14I 221 CCAAGG C^C[AT][AT]GG
EcoT22I 1601 ATGCAT ATGCA^T
HaeII 201 GGCGCC [GA]GCGC^[CT]
Hin1I 201 GGCGCC G[GA]^CG[CT]C
HindIII 1711 AAGCTT A^AGCTT
HpaI 1590 GTTAAC GTT^AAC
HphI 1173 GGTGA GGTGA(8/7)
KasI 201 GGCGCC G^GCGCC
MflI 2004 AGATCC [GA]^GATC[CT]
NarI 201 GGCGCC GG^CGCC
NdeI 1108 CATATG CA^TATG
NsiI 1601 ATGCAT ATGCA^T
PflMI 1656 CCAGTTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 728 ACCGGT A^CCGGT
PpuMI 47 GGGTCCC [GA]G^G[AT]CC[CT]
PshBI 357 ATTAAT AT^TAAT
PstI 214 CTGCAG CTGCA^G
SapI 844 GAAGAGC GCTCTTC(1/4)
ScaI 1440 AGTACT AGT^ACT
SexAI 1628 ACCTGGT A^CC[AT]GGT
SfoI 201 GGCGCC GGC^GCC
StuI 77 AGGCCT AGG^CCT
StyI 221 CCAAGG C^C[AT][AT]GG
TaqI 561 TCGA T^CGA
TthHB8I 561 TCGA T^CGA
Van91I 1656 CCAGTTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 675 CCAGCCCTTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 2004 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 178 GGTACC G^GTACC
1632 GGTACC
AccII 165 CGCG CG^CG
209 CGCG
AlwNI 254 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
411 CAGTGGCTG
Asp718I 178 GGTACC G^GTACC
1632 GGTACC
AvaII 48 GGTCC G^G[AT]CC
750 GGTCC
BfaI 1006 CTAG C^TAG
1451 CTAG
BsmAI 124 GAGAC GTCTC(1/5)
586 GAGAC
BspMI 181 ACCTGC ACCTGC(4/8)
747 GCAGGT
BsrFI 137 ACCGGC [GA]^CCGG[CT]
728 ACCGGT
BstUI 165 CGCG CG^CG
209 CGCG
Cfr10I 137 ACCGGC [GA]^CCGG[CT]
728 ACCGGT
DpnII 755 GATC ^GATC
2005 GATC
DraII 47 GGGTCCC [GA]G^G[ACGT]CC[CT]
1142 GGGCCCC
EcoO109I 47 GGGTCCC [GA]G^G[ACGT]CC[CT]
1142 GGGCCCC
HapII 138 CCGG C^CGG
729 CCGG
HpaII 138 CCGG C^CGG
729 CCGG
KpnI 178 GGTACC GGTAC^C
1632 GGTACC
MaeI 1006 CTAG C^TAG
1451 CTAG
MboI 755 GATC ^GATC
2005 GATC
MslI 169 CACCTCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1699 CACCTTCATG
MspA1I 554 CAGCTG C[AC]G^C[GT]G
1639 CAGCTG
MspI 138 CCGG C^CGG
729 CCGG
MvnI 165 CGCG CG^CG
209 CGCG
NdeII 755 GATC ^GATC
2005 GATC
NspI 469 ACATGC [GA]CATG^[CT]
618 ACATGC
PvuII 554 CAGCTG CAG^CTG
1639 CAGCTG
Sau3AI 755 GATC ^GATC
2005 GATC
Tsp45I 107 GTCAC ^GT[GC]AC
636 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AfaI AluI BanI BbsI
BbvI BpmI BpuAI BsaJI BseRI
BsgI BsiYI BslI BsmFI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI EarI EcoRII FauI
Fnu4HI FokI HaeIII HhaI HinP1I
HincII HindII HinfI ItaI Ksp632I
MaeII MaeIII MboII MnlI MseI
MvaI MwoI NlaIII NlaIV PleI
RsaI Sau96I ScrFI SfaNI SfcI
TfiI Tru9I TseI Tsp509I TspRI