Restriction map for
pF1KSDA0017
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# NO CUT ENZYMES ( 96 enzymes) #
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AatII AccII AclI AcyI AflII
AflIII AgeI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
AviII AvrII BbeI BbrPI BciVI
BcnI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaHI BsiEI BsiWI
BspLU11I BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstZ17I Bsu36I
CelII CfoI Cfr10I CpoI DraIII
EagI EclXI Eco47III Eco52I Eco81I
EcoRI EcoT22I FseI FspI HaeII
HhaI Hin1I HinP1I HpaI KasI
KspI MluI MvnI NaeI NarI
NciI NgoMIV NheI NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacII SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I StuI SwaI XbaI XmaCI
XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 2075 GGTACC G^GTACC
AccI 1388 GTCGAC GT^[AC][GT]AC
AccIII 3632 TCCGGA T^CCGGA
AhdI 1380 GACAGTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 710 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 2075 GGTACC G^GTACC
AspEI 1380 GACAGTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 2558 GAGCTC G[AT]GC[AT]^C
BcgI 1797 GCAGCGGTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3272 TGATCA T^GATCA
BglII 107 AGATCT A^GATCT
BsaBI 3199 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 3632 TCCGGA T^CCGGA
BseRI 1679 GAGGAG GAGGAG(10/8)
BsiHKAI 2558 GAGCTC G[AT]GC[AT]^C
Bsp1407I 8 TGTACA T^GTACA
BspDI 888 ATCGAT AT^CGAT
BspEI 3632 TCCGGA T^CCGGA
BspHI 3275 TCATGA T^CATGA
BsrDI 2573 GCAATG GCAATG(2/0)
BsrGI 8 TGTACA T^GTACA
ClaI 888 ATCGAT AT^CGAT
DraI 648 TTTAAA TTT^AAA
Eam1105I 1380 GACAGTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 3272 TGATCA T^GATCA
HgaI 1386 GCGTC GACGC(5/10)
HincII 1388 GTCGAC GT[CT]^[GA]AC
HindII 1388 GTCGAC GT[CT]^[GA]AC
HindIII 2785 AAGCTT A^AGCTT
KpnI 2075 GGTACC GGTAC^C
MamI 3199 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1628 CAATTG C^AATTG
MroI 3632 TCCGGA T^CCGGA
MunI 1628 CAATTG C^AATTG
NcoI 3551 CCATGG C^CATGG
NdeI 3156 CATATG CA^TATG
PaeR7I 3622 CTCGAG C^TCGAG
PflMI 3423 CCAGCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 247 GACTCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3275 TCATGA T^CATGA
SacI 2558 GAGCTC GAGCT^C
SalI 1388 GTCGAC G^TCGAC
SapI 2100 GCTCTTC GCTCTTC(1/4)
ScaI 741 AGTACT AGT^ACT
SexAI 1356 ACCTGGT A^CC[AT]GGT
SphI 1886 GCATGC GCATG^C
SspBI 8 TGTACA T^GTACA
Van91I 3423 CCAGCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 309 CCAAGAAACCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3622 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 27 enzymes) #
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Asp700I 59 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2097 GAAGCTCTTC
AvaI 2251 CCCGAG C^[CT]CG[GA]G
3622 CTCGAG
BanI 217 GGCACC G^G[CT][GA]CC
2075 GGTACC
BanII 1971 GGGCTC G[GA]GC[CT]^C
2558 GAGCTC
BbvCI 3480 CCTCAGC CCTCAGC(-5/-2)
2022 GCTGAGG
BfaI 637 CTAG C^TAG
1447 CTAG
BmyI 1971 GGGCTC G[AGT]GC[ACT]^C
2558 GAGCTC
BsaI 3527 GAGACC GGTCTC(1/5)
1802 GGTCTC
BsaWI 185 TCCGGT [AT]^CCGG[AT]
3632 TCCGGA
BsmBI 3576 CGTCTC CGTCTC(1/5)
3295 GAGACG
BsoBI 2251 CCCGAG C^[CT]CG[GA]G
3622 CTCGAG
Bsp1286I 1971 GGGCTC G[AGT]GC[ACT]^C
2558 GAGCTC
BsrBI 2151 CCGCTC CCGCTC(-3/-3)
2491 GAGCGG
BstEII 784 GGTGACC G^GT[ACGT]ACC
1360 GGTAACC
BstPI 784 GGTGACC G^GT[ACGT]ACC
1360 GGTAACC
EarI 2101 CTCTTC CTCTTC(1/4)
2836 GAAGAG
EcoNI 841 CCTGAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1277 CCTGTGGTAGG
EcoO65I 784 GGTGACC G^GT[ACGT]ACC
1360 GGTAACC
Ksp632I 2101 CTCTTC CTCTTC(1/4)
2836 GAAGAG
MaeI 637 CTAG C^TAG
1447 CTAG
MaeII 3397 ACGT A^CGT
3575 ACGT
NspI 1886 GCATGC [GA]CATG^[CT]
3426 GCATGT
PstI 443 CTGCAG CTGCA^G
2341 CTGCAG
PvuII 1717 CAGCTG CAG^CTG
2641 CAGCTG
SmlI 1324 CTTGAG C^T[CT][GA]AG
3622 CTCGAG
SspI 983 AATATT AAT^ATT
1996 AATATT
XmnI 59 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2097 GAAGCTCTTC
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# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspI AvaII BalI BamHI
BbsI BbvI BpmI BpuAI BsaJI
BsgI BsiYI BslI BsmAI BsmFI
BsmI BspMI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII DraII DrdI DsaI
EaeI EcoO109I EcoRII EcoRV EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
PflFI PleI PpuMI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I XhoII