Restriction map for
pF1KSDA0027
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BbeI
BbrPI BbvCI BciVI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaBI
BsaHI BsaI BseAI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstZ17I Bsu36I CelII ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRI
EcoT14I EcoT22I FspI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MluI MroI MslI
NarI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SphI Sse8387I SspBI SspI StuI
StyI SwaI Tth111I Van91I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccII 93 CGCG CG^CG
AfaI 210 GTAC GT^AC
AlwNI 821 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 931 GTGCTC G[AT]GC[AT]^C
BalI 1061 TGGCCA TGG^CCA
BamHI 701 GGATCC G^GATCC
BanI 961 GGCACC G^G[CT][GA]CC
BbsI 157 GTCTTC GAAGAC(2/6)
BclI 479 TGATCA T^GATCA
BcnI 228 CCCGG CC^[GC]GG
BpuAI 157 GTCTTC GAAGAC(2/6)
BsaAI 369 TACGTG [CT]AC^GT[GA]
BsaWI 38 ACCGGA [AT]^CCGG[AT]
BsgI 488 CTGCAC GTGCAG(16/14)
BsiHKAI 931 GTGCTC G[AT]GC[AT]^C
BspHI 848 TCATGA T^CATGA
BspMI 107 ACCTGC ACCTGC(4/8)
BsrBI 55 GAGCGG CCGCTC(-3/-3)
BstEII 189 GGTGACC G^GT[ACGT]ACC
BstPI 189 GGTGACC G^GT[ACGT]ACC
BstUI 93 CGCG CG^CG
BstYI 701 GGATCC [GA]^GATC[CT]
DraIII 366 CACTACGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 473 CCACGG C^C[GA][CT]GG
EarI 120 GAAGAG CTCTTC(1/4)
EcoNI 936 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 189 GGTGACC G^GT[ACGT]ACC
EcoRV 830 GATATC GAT^ATC
FbaI 479 TGATCA T^GATCA
FseI 945 GGCCGGCC GGCCGG^CC
HgaI 91 GACGC GACGC(5/10)
Ksp632I 120 GAAGAG CTCTTC(1/4)
MfeI 281 CAATTG C^AATTG
MflI 701 GGATCC [GA]^GATC[CT]
MluNI 1061 TGGCCA TGG^CCA
MscI 1061 TGGCCA TGG^CCA
MunI 281 CAATTG C^AATTG
MvnI 93 CGCG CG^CG
NciI 228 CCCGG CC^[GC]GG
PleI 661 GACTC GAGTC(4/5)
PshAI 150 GACGTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 112 CAGCTG CAG^CTG
RcaI 848 TCATGA T^CATGA
RsaI 210 GTAC GT^AC
SpeI 396 ACTAGT A^CTAGT
XcmI 758 CCAGCAAGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 701 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflIII 221 ACGTGT A^C[GA][CT]GT
370 ACGTGT
Asp700I 219 GAACGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1077 GAAGGAGTTC
BanII 525 GGGCTC G[GA]GC[CT]^C
1068 GAGCCC
BcgI 101 CGAGCGACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
119 CGAAGAGACTGC
BseRI 22 GAGGAG GAGGAG(10/8)
502 GAGGAG
BsmBI 297 CGTCTC CGTCTC(1/5)
803 CGTCTC
BsmFI 1019 GGGAC GGGAC(10/14)
755 GTCCC
BsrDI 642 CATTGC GCAATG(2/0)
729 CATTGC
BsrFI 62 GCCGGC [GA]^CCGG[CT]
946 GCCGGC
BsrI 734 CCAGT ACTGG(1/-1)
972 CCAGT
BstXI 545 CCAACATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
734 CCAGTCATGTGG
Cfr10I 62 GCCGGC [GA]^CCGG[CT]
946 GCCGGC
DraII 578 GGGTCCT [GA]G^G[ACGT]CC[CT]
776 AGGTCCT
EaeI 470 CGGCCA [CT]^GGCC[GA]
1061 TGGCCA
EcoO109I 578 GGGTCCT [GA]G^G[ACGT]CC[CT]
776 AGGTCCT
HaeII 541 AGCGCC [GA]GCGC^[CT]
1043 AGCGCC
MboII 120 GAAGA GAAGA(8/7)
158 TCTTC
MseI 376 TTAA T^TAA
869 TTAA
MspA1I 112 CAGCTG C[AC]G^C[GT]G
808 CCGCTG
NaeI 62 GCCGGC GCC^GGC
946 GCCGGC
NgoMIV 62 GCCGGC G^CCGGC
946 GCCGGC
PpuMI 578 GGGTCCT [GA]G^G[AT]CC[CT]
776 AGGTCCT
TfiI 321 GATTC G^A[AT]TC
854 GAATC
Tru9I 376 TTAA T^TAA
869 TTAA
Tsp509I 282 AATT ^AATT
378 AATT
XmnI 219 GAACGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1077 GAAGGAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AluI AlwI AvaI AvaII
BbvI BfaI BmyI BpmI BsaJI
BsiYI BslI BsmAI BsoBI Bsp1286I
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MnlI
MspI MvaI MwoI NdeII NlaIII
NlaIV PstI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TseI Tsp45I
TspRI TthHB8I