Restriction map for
pF1KSDA0031
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# NO CUT ENZYMES ( 69 enzymes) #
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AatII AclI AflII AhdI Alw44I
ApaLI AscI AspEI AspI AvrII
BamHI BfrI BglI BlnI BsiEI
BsiWI BsmI BspDI BspMI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI EagI
Eam1105I EclXI Eco52I EcoO65I EcoRV
FseI HpaI KspI MluI NaeI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PmeI
PpuMI Psp1406I PvuI RsrII SacII
SalI SbfI SexAI SfiI SfuI
SnaBI SpeI Sse8387I SspI StuI
SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 61 enzymes) #
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Acc65I 1607 GGTACC G^GTACC
AccI 2446 GTCTAC GT^[AC][GT]AC
AccII 1058 CGCG CG^CG
AccIII 2660 TCCGGA T^CCGGA
AcyI 1330 GGCGCC G[GA]^CG[CT]C
AgeI 1360 ACCGGT A^CCGGT
AocI 223 CCTGAGG CC^T[ACGT]AGG
Aor51HI 476 AGCGCT AGC^GCT
ApaI 2328 GGGCCC GGGCC^C
Asp700I 2344 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1607 GGTACC G^GTACC
BbeI 1330 GGCGCC GGCGC^C
BbrPI 2566 CACGTG CAC^GTG
BbvCI 694 GCTGAGG CCTCAGC(-5/-2)
BcgI 2849 GCAAATTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3 GGATAC GTATCC(6/5)
BlpI 2065 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2065 GCTGAGC GC^T[ACGT]AGC
BsaHI 1330 GGCGCC G[GA]^CG[CT]C
BseAI 2660 TCCGGA T^CCGGA
BsmFI 2676 GGGAC GGGAC(10/14)
BspEI 2660 TCCGGA T^CCGGA
BsrBI 718 GAGCGG CCGCTC(-3/-3)
BsrDI 1709 GCAATG GCAATG(2/0)
BstUI 1058 CGCG CG^CG
Bsu36I 223 CCTGAGG CC^T[ACGT]AGG
CelII 2065 GCTGAGC GC^T[ACGT]AGC
DrdI 1969 GACCCAGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 476 AGCGCT AGC^GCT
Eco81I 223 CCTGAGG CC^T[ACGT]AGG
EcoRI 2794 GAATTC G^AATTC
EcoT22I 1921 ATGCAT ATGCA^T
Hin1I 1330 GGCGCC G[GA]^CG[CT]C
HindIII 2615 AAGCTT A^AGCTT
KasI 1330 GGCGCC G^GCGCC
KpnI 1607 GGTACC GGTAC^C
MfeI 1673 CAATTG C^AATTG
MroI 2660 TCCGGA T^CCGGA
MspA1I 647 CAGCTG C[AC]G^C[GT]G
MunI 1673 CAATTG C^AATTG
MvnI 1058 CGCG CG^CG
NarI 1330 GGCGCC GG^CGCC
NcoI 417 CCATGG C^CATGG
NsiI 1921 ATGCAT ATGCA^T
PflMI 837 CCACATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1360 ACCGGT A^CCGGT
PmaCI 2566 CACGTG CAC^GTG
PmlI 2566 CACGTG CAC^GTG
PshAI 558 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 647 CAGCTG CAG^CTG
SapI 2269 GCTCTTC GCTCTTC(1/4)
ScaI 191 AGTACT AGT^ACT
SfoI 1330 GGCGCC GGC^GCC
SgrAI 1359 CACCGGTG C[GA]^CCGG[CT]G
SmaI 2789 CCCGGG CCC^GGG
SphI 732 GCATGC GCATG^C
Van91I 837 CCACATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2225 CCAACATTCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 2789 CCCGGG C^CCGGG
XmaI 2789 CCCGGG C^CCGGG
XmnI 2344 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 38 enzymes) #
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AlwNI 755 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
942 CAGCATCTG
AseI 861 ATTAAT AT^TAAT
1000 ATTAAT
AsnI 861 ATTAAT AT^TAAT
1000 ATTAAT
AvaI 1703 CCCGAG C^[CT]CG[GA]G
2789 CCCGGG
AviII 656 TGCGCA TGC^GCA
1820 TGCGCA
BalI 1601 TGGCCA TGG^CCA
2549 TGGCCA
BfaI 1085 CTAG C^TAG
1186 CTAG
BsaAI 2121 TACGTG [CT]AC^GT[GA]
2566 CACGTG
BsaBI 1950 GATAGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2167 GATTGGGATC
BsaI 85 GAGACC GGTCTC(1/5)
232 GAGACC
BsaWI 1360 ACCGGT [AT]^CCGG[AT]
2660 TCCGGA
BsmBI 2526 CGTCTC CGTCTC(1/5)
1990 GAGACG
BsoBI 1703 CCCGAG C^[CT]CG[GA]G
2789 CCCGGG
Bsp1407I 1442 TGTACA T^GTACA
2603 TGTACA
BspHI 141 TCATGA T^CATGA
2798 TCATGA
BsrFI 782 ACCGGC [GA]^CCGG[CT]
1360 ACCGGT
BsrGI 1442 TGTACA T^GTACA
2603 TGTACA
BstXI 1853 CCACCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2225 CCAACATTCTGG
Cfr10I 782 ACCGGC [GA]^CCGG[CT]
1360 ACCGGT
DraIII 759 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
838 CACATTGTG
FauI 2810 CCCGC CCCGC(4/6)
2411 GCGGG
FspI 656 TGCGCA TGC^GCA
1820 TGCGCA
HaeII 476 AGCGCT [GA]GCGC^[CT]
1330 GGCGCC
HgaI 1056 GACGC GACGC(5/10)
2525 GCGTC
HincII 1783 GTCAAC GT[CT]^[GA]AC
1828 GTCAAC
HindII 1783 GTCAAC GT[CT]^[GA]AC
1828 GTCAAC
MaeI 1085 CTAG C^TAG
1186 CTAG
MamI 1950 GATAGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2167 GATTGGGATC
MluNI 1601 TGGCCA TGG^CCA
2549 TGGCCA
MscI 1601 TGGCCA TGG^CCA
2549 TGGCCA
PshBI 861 ATTAAT AT^TAAT
1000 ATTAAT
PstI 2516 CTGCAG CTGCA^G
2531 CTGCAG
RcaI 141 TCATGA T^CATGA
2798 TCATGA
SacI 379 GAGCTC GAGCT^C
1900 GAGCTC
SfcI 2516 CTGCAG C^T[GA][CT]AG
2531 CTGCAG
SmlI 2014 CTCAAG C^T[CT][GA]AG
2074 CTTGAG
SspBI 1442 TGTACA T^GTACA
2603 TGTACA
TfiI 52 GATTC G^A[AT]TC
2349 GATTC
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# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AspHI AvaII BanI
BanII BbsI BbvI BclI BcnI
BglII BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BspLU11I BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FbaI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII