Restriction map for
pF1KSDA0039
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# NO CUT ENZYMES ( 152 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BpmI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmI
BsoBI BspDI BspEI BspHI BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I CfoI Cfr10I ClaI
CpoI DraIII DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
FauI FbaI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HindIII HpaII KasI KpnI KspI
MaeII MamI MfeI MflI MluNI
MroI MscI MspI MunI NaeI
NarI NciI NcoI NdeI NgoMIV
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspI StyI SwaI TaqI Tth111I
TthHB8I Van91I XbaI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 1210 GTCTAC GT^[AC][GT]AC
AccII 1153 CGCG CG^CG
AciI 4 GCGG CCGC(-3/-1)
ApaI 1323 GGGCCC GGGCC^C
Asp700I 1003 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 681 GGCACC G^G[CT][GA]CC
BlpI 75 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 75 GCTGAGC GC^T[ACGT]AGC
BsmBI 1064 CGTCTC CGTCTC(1/5)
Bsp1407I 299 TGTACA T^GTACA
BspLU11I 482 ACATGT A^CATGT
BsrGI 299 TGTACA T^GTACA
BstAPI 826 GCAACTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1153 CGCG CG^CG
CelII 75 GCTGAGC GC^T[ACGT]AGC
DpnII 57 GATC ^GATC
DraI 739 TTTAAA TTT^AAA
DrdI 31 GACGAGTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 659 ATGCAT ATGCA^T
HincII 100 GTTAAC GT[CT]^[GA]AC
HindII 100 GTTAAC GT[CT]^[GA]AC
HpaI 100 GTTAAC GTT^AAC
HphI 1042 TCACC GGTGA(8/7)
MboI 57 GATC ^GATC
MluI 1152 ACGCGT A^CGCGT
MspA1I 413 CAGCTG C[AC]G^C[GT]G
MvnI 1153 CGCG CG^CG
NdeII 57 GATC ^GATC
NheI 273 GCTAGC G^CTAGC
NsiI 659 ATGCAT ATGCA^T
PshAI 1364 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 413 CAGCTG CAG^CTG
Sau3AI 57 GATC ^GATC
SbfI 832 CCTGCAGG CCTGCA^GG
SmlI 1122 CTCAAG C^T[CT][GA]AG
Sse8387I 832 CCTGCAGG CCTGCA^GG
SspBI 299 TGTACA T^GTACA
StuI 837 AGGCCT AGG^CCT
XcmI 566 CCAAAGGAATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1003 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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AflIII 482 ACATGT A^C[GA][CT]GT
1152 ACGCGT
AspHI 428 GAGCTC G[AT]GC[AT]^C
1119 GAGCTC
AvaII 6 GGACC G^G[AT]CC
45 GGACC
BbsI 1257 GAAGAC GAAGAC(2/6)
450 GTCTTC
BpuAI 1257 GAAGAC GAAGAC(2/6)
450 GTCTTC
BsaJI 685 CCAGGG C^C[ACGT][ACGT]GG
1021 CCTGGG
BsgI 1227 CTGCAC GTGCAG(16/14)
410 GTGCAG
BsiHKAI 428 GAGCTC G[AT]GC[AT]^C
1119 GAGCTC
BsmFI 1336 GGGAC GGGAC(10/14)
421 GTCCC
BspMI 537 ACCTGC ACCTGC(4/8)
549 GCAGGT
BsrI 1378 ACTGG ACTGG(1/-1)
1081 CCAGT
DraII 1322 AGGGCCC [GA]G^G[ACGT]CC[CT]
1323 GGGCCCC
EarI 444 CTCTTC CTCTTC(1/4)
1240 GAAGAG
EcoO109I 1322 AGGGCCC [GA]G^G[ACGT]CC[CT]
1323 GGGCCCC
Ksp632I 444 CTCTTC CTCTTC(1/4)
1240 GAAGAG
NspI 171 GCATGT [GA]CATG^[CT]
482 ACATGT
SacI 428 GAGCTC GAGCT^C
1119 GAGCTC
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# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
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AcsI AfaI AluI AlwNI ApoI
BanII BbvI BfaI BmyI BseRI
BsiYI BslI BsmAI Bsp1286I BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
EcoRII Fnu4HI FokI HaeIII HinfI
ItaI MaeI MaeIII MboII MnlI
MseI MslI MvaI MwoI NlaIII
NlaIV PleI PstI RsaI Sau96I
ScrFI SfaNI SfcI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI