Restriction map for
pF1KSDA0046
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# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
AocI Aor51HI ApaI AscI AseI
AsnI AspEI AspI AvrII BamHI
BclI BfrI BglII BlnI BlpI
Bpu1102I BsiWI Bsp1407I BspDI BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoT22I FbaI FseI HincII HindII
HindIII HpaI MfeI MluI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PshBI Psp1406I
PvuI PvuII RsrII SacI SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
XhoI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 1834 GTCTAC GT^[AC][GT]AC
AccIII 530 TCCGGA T^CCGGA
AcyI 765 GGCGCC G[GA]^CG[CT]C
AflIII 2063 ACATGT A^C[GA][CT]GT
Alw44I 1096 GTGCAC G^TGCAC
ApaLI 1096 GTGCAC G^TGCAC
Asp700I 1929 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1874 TGCGCA TGC^GCA
BbeI 765 GGCGCC GGCGC^C
BbvCI 2583 GCTGAGG CCTCAGC(-5/-2)
BcgI 2486 CGATGGGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 473 GGATAC GTATCC(6/5)
BfaI 793 CTAG C^TAG
BsaBI 154 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 765 GGCGCC G[GA]^CG[CT]C
BsaWI 530 TCCGGA [AT]^CCGG[AT]
BseAI 530 TCCGGA T^CCGGA
BsiEI 2239 CGGTCG CG[GA][CT]^CG
BsmI 1192 GAATGC GAATGC(1/-1)
BspEI 530 TCCGGA T^CCGGA
BspHI 1937 TCATGA T^CATGA
BspLU11I 2063 ACATGT A^CATGT
BsrFI 453 GCCGGT [GA]^CCGG[CT]
BstEII 1149 GGTAACC G^GT[ACGT]ACC
BstPI 1149 GGTAACC G^GT[ACGT]ACC
BstYI 671 AGATCC [GA]^GATC[CT]
Cfr10I 453 GCCGGT [GA]^CCGG[CT]
EcoO65I 1149 GGTAACC G^GT[ACGT]ACC
EcoRI 1263 GAATTC G^AATTC
EcoRV 1729 GATATC GAT^ATC
FspI 1874 TGCGCA TGC^GCA
HgaI 2376 GACGC GACGC(5/10)
Hin1I 765 GGCGCC G[GA]^CG[CT]C
KasI 765 GGCGCC G^GCGCC
MaeI 793 CTAG C^TAG
MamI 154 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 671 AGATCC [GA]^GATC[CT]
MroI 530 TCCGGA T^CCGGA
NarI 765 GGCGCC GG^CGCC
RcaI 1937 TCATGA T^CATGA
SfoI 765 GGCGCC GGC^GCC
SmaI 1997 CCCGGG CCC^GGG
SmlI 322 CTCAAG C^T[CT][GA]AG
XbaI 792 TCTAGA T^CTAGA
XhoII 671 AGATCC [GA]^GATC[CT]
XmaCI 1997 CCCGGG C^CCGGG
XmaI 1997 CCCGGG C^CCGGG
XmnI 1929 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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Acc65I 778 GGTACC G^GTACC
2348 GGTACC
AcsI 1263 GAATTC [GA]^AATT[CT]
2566 GAATTT
AlwNI 1855 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
2122 CAGATGCTG
ApoI 1263 GAATTC [GA]^AATT[CT]
2566 GAATTT
Asp718I 778 GGTACC G^GTACC
2348 GGTACC
BalI 1313 TGGCCA TGG^CCA
1688 TGGCCA
BbrPI 828 CACGTG CAC^GTG
1735 CACGTG
BsaAI 828 CACGTG [CT]AC^GT[GA]
1735 CACGTG
BsrBI 734 GAGCGG CCGCTC(-3/-3)
2200 GAGCGG
BstAPI 35 GCAGGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2118 GCAGCAGATGC
HaeII 765 GGCGCC [GA]GCGC^[CT]
2296 AGCGCC
KpnI 778 GGTACC GGTAC^C
2348 GGTACC
KspI 1917 CCGCGG CCGC^GG
2344 CCGCGG
MluNI 1313 TGGCCA TGG^CCA
1688 TGGCCA
MscI 1313 TGGCCA TGG^CCA
1688 TGGCCA
NcoI 200 CCATGG C^CATGG
2192 CCATGG
NspI 1426 GCATGT [GA]CATG^[CT]
2063 ACATGT
PflMI 1854 CCAGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2361 CCACGACCTGG
PmaCI 828 CACGTG CAC^GTG
1735 CACGTG
PmlI 828 CACGTG CAC^GTG
1735 CACGTG
PshAI 1199 GACAGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1827 GACCTACGTC
SacII 1917 CCGCGG CCGC^GG
2344 CCGCGG
SapI 582 GCTCTTC GCTCTTC(1/4)
1356 GCTCTTC
Tsp509I 1264 AATT ^AATT
2567 AATT
Van91I 1854 CCAGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2361 CCACGACCTGG
XcmI 1802 CCAGCGACATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2176 CCAAGTGAAGACTGG
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# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaI AvaII BanI BanII
BbsI BbvI BcnI BglI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
BstXI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I TspRI TthHB8I